The influence of Methylphenidate on the development of the forensically significant blow fly Chrysomya chloropyga (Diptera: Calliphoridae) in the Western Cape Province

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dc.contributor.advisorHeyns, Mariseen_ZA
dc.contributor.advisorDavies, Bronwenen_ZA
dc.contributor.authorVisser, Hartwigen_ZA
dc.date.accessioned2016-07-07T09:51:26Z
dc.date.available2016-07-07T09:51:26Z
dc.date.issued2016en_ZA
dc.description.abstractForensic entomologists rely on insect development and successional data to estimate the post-mortem interval (PMI). Ante-mortem drug use prior to an individual's death may result in drug transmission to feeding insects and subsequent alteration of their development, thereby altering PMI estimates. This study investigated the influence of Ritalin (methylphenidate hydrochloride) (MPH) on the development of Chrysomya chloropyga. C. chloropyga larvae were reared on pig liver treated with MPH and exhibited a trend of expedited larval development and prolonged pupal development. Conservatively, the results suggest that MPH may expedite the larval stage by up to 17 hours and prolong the pupal stage by up to 16 hours. These preliminary findings suggest that, at the concentration investigated, MPH may alter the duration of C. chloropyga developmental stages, and consequently PMI estimates if MPH is not detected or its effects not considered. Furthermore, MPH was detected in both frozen and ethanol preserved specimens. MPH could still be detected from treated larvae, after 3.5 days incubation at ~30°C. This may suggest an improved stability of MPH in insects. Moreover, detection of MPH from ethanol preserved specimens suggests the qualitative toxicological utility of specimens maintained in this preservation liquid, despite stability and self-extraction concerns. These are the first entomotoxicological data on MPH generated for blow flies local to the Western Cape, South Africa.en_ZA
dc.identifier.apacitationVisser, H. (2016). <i>The influence of Methylphenidate on the development of the forensically significant blow fly Chrysomya chloropyga (Diptera: Calliphoridae) in the Western Cape Province</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Forensic Medicine and Toxicology. Retrieved from http://hdl.handle.net/11427/20247en_ZA
dc.identifier.chicagocitationVisser, Hartwig. <i>"The influence of Methylphenidate on the development of the forensically significant blow fly Chrysomya chloropyga (Diptera: Calliphoridae) in the Western Cape Province."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Forensic Medicine and Toxicology, 2016. http://hdl.handle.net/11427/20247en_ZA
dc.identifier.citationVisser, H. 2016. The influence of Methylphenidate on the development of the forensically significant blow fly Chrysomya chloropyga (Diptera: Calliphoridae) in the Western Cape Province. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Visser, Hartwig AB - Forensic entomologists rely on insect development and successional data to estimate the post-mortem interval (PMI). Ante-mortem drug use prior to an individual's death may result in drug transmission to feeding insects and subsequent alteration of their development, thereby altering PMI estimates. This study investigated the influence of Ritalin (methylphenidate hydrochloride) (MPH) on the development of Chrysomya chloropyga. C. chloropyga larvae were reared on pig liver treated with MPH and exhibited a trend of expedited larval development and prolonged pupal development. Conservatively, the results suggest that MPH may expedite the larval stage by up to 17 hours and prolong the pupal stage by up to 16 hours. These preliminary findings suggest that, at the concentration investigated, MPH may alter the duration of C. chloropyga developmental stages, and consequently PMI estimates if MPH is not detected or its effects not considered. Furthermore, MPH was detected in both frozen and ethanol preserved specimens. MPH could still be detected from treated larvae, after 3.5 days incubation at ~30°C. This may suggest an improved stability of MPH in insects. Moreover, detection of MPH from ethanol preserved specimens suggests the qualitative toxicological utility of specimens maintained in this preservation liquid, despite stability and self-extraction concerns. These are the first entomotoxicological data on MPH generated for blow flies local to the Western Cape, South Africa. DA - 2016 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2016 T1 - The influence of Methylphenidate on the development of the forensically significant blow fly Chrysomya chloropyga (Diptera: Calliphoridae) in the Western Cape Province TI - The influence of Methylphenidate on the development of the forensically significant blow fly Chrysomya chloropyga (Diptera: Calliphoridae) in the Western Cape Province UR - http://hdl.handle.net/11427/20247 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/20247
dc.identifier.vancouvercitationVisser H. The influence of Methylphenidate on the development of the forensically significant blow fly Chrysomya chloropyga (Diptera: Calliphoridae) in the Western Cape Province. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Forensic Medicine and Toxicology, 2016 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/20247en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Forensic Medicine and Toxicologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherBiomedical Forensic Scienceen_ZA
dc.titleThe influence of Methylphenidate on the development of the forensically significant blow fly Chrysomya chloropyga (Diptera: Calliphoridae) in the Western Cape Provinceen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMPhilen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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