Identification of phytoplasmas associated with cultivated and ornamental plants in Kerman Province, Iran

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dc.contributor.authorTazehkand, Susan Asghari
dc.contributor.authorPour, Akbar Hossein
dc.contributor.authorHeydarnejad, Jahangir
dc.contributor.authorVarsani, Arvind
dc.contributor.authorMassumi, Hossain
dc.date.accessioned2016-07-26T11:44:18Z
dc.date.available2016-07-26T11:44:18Z
dc.date.issued2010
dc.date.updated2016-07-26T11:43:20Z
dc.description.abstractHossain Kerman Province is a major agricultural centre in south-eastern Iran and an increase in agricultural activities results in an increase in disease. We report phytoplasmal infections in Iran of five plant species; spinach (Spinacia oleracea L.), sunflower (Helianthus annuus L.), canola (Brassica napus L.), cucumber (Cucumis sativus L.) and Aegean wallflower (Erysimum cheiri (L.) Crantz; Family Brassicaceae) using in silico restriction fragment length polymorphism and sequence analysis. Amplicons of approximately 300 bp were amplified using polymerase chain reaction ampli- fication with universal P3 ⁄ P7 primer pair. The ampli- fied products were cloned and sequenced. On the basis of in silico restriction analysis of the amplicon digested with 17 distinct restriction enzymes and 16 ⁄ 23S spacer region sequence, Erysimum and cucumber phyllodies (EPh and CuPh2, respectively) were 100% identical and showed closest similarity with members of the peanut witches-broom group (16SrII). Whereas spinach yellows (SpY) and canola phyllody (CaPh) revealed closest homology with phytoplasmas of the aster yellows group (AY) 16SrI. Mixed infections of the SuWB sample were confirmed in which two different phytoplasmas belonging to 16SrII and 16SrVI groups were found. This is the first report of phytoplasmal infection of Aegean wallflower (EPh) caused by a phytoplasma belonging to the 16SrII group. While spinach phytoplasmas have been isolated in the past; however, our isolate from spinach belonging to the 16SrI group is the first spinach isolate from Iran.en_ZA
dc.identifierhttp://dx.doi.org/10.1111/j.1439-0434.2010.01682.x
dc.identifier.apacitationTazehkand, S. A., Pour, A. H., Heydarnejad, J., Varsani, A., & Massumi, H. (2010). Identification of phytoplasmas associated with cultivated and ornamental plants in Kerman Province, Iran. <i>Journal of Phytopathology</i>, http://hdl.handle.net/11427/20763en_ZA
dc.identifier.chicagocitationTazehkand, Susan Asghari, Akbar Hossein Pour, Jahangir Heydarnejad, Arvind Varsani, and Hossain Massumi "Identification of phytoplasmas associated with cultivated and ornamental plants in Kerman Province, Iran." <i>Journal of Phytopathology</i> (2010) http://hdl.handle.net/11427/20763en_ZA
dc.identifier.citationTazehkand, S. A., Pour, A. H., Heydarnejad, J., Varsani, A., & Massumi, H. (2010). Identification of phytoplasmas associated with cultivated and ornamental plants in Kerman province, Iran. Journal of Phytopathology, 158(11‐12), 713-720.en_ZA
dc.identifier.issn0931-1785en_ZA
dc.identifier.ris TY - Journal Article AU - Tazehkand, Susan Asghari AU - Pour, Akbar Hossein AU - Heydarnejad, Jahangir AU - Varsani, Arvind AU - Massumi, Hossain AB - Hossain Kerman Province is a major agricultural centre in south-eastern Iran and an increase in agricultural activities results in an increase in disease. We report phytoplasmal infections in Iran of five plant species; spinach (Spinacia oleracea L.), sunflower (Helianthus annuus L.), canola (Brassica napus L.), cucumber (Cucumis sativus L.) and Aegean wallflower (Erysimum cheiri (L.) Crantz; Family Brassicaceae) using in silico restriction fragment length polymorphism and sequence analysis. Amplicons of approximately 300 bp were amplified using polymerase chain reaction ampli- fication with universal P3 ⁄ P7 primer pair. The ampli- fied products were cloned and sequenced. On the basis of in silico restriction analysis of the amplicon digested with 17 distinct restriction enzymes and 16 ⁄ 23S spacer region sequence, Erysimum and cucumber phyllodies (EPh and CuPh2, respectively) were 100% identical and showed closest similarity with members of the peanut witches-broom group (16SrII). Whereas spinach yellows (SpY) and canola phyllody (CaPh) revealed closest homology with phytoplasmas of the aster yellows group (AY) 16SrI. Mixed infections of the SuWB sample were confirmed in which two different phytoplasmas belonging to 16SrII and 16SrVI groups were found. This is the first report of phytoplasmal infection of Aegean wallflower (EPh) caused by a phytoplasma belonging to the 16SrII group. While spinach phytoplasmas have been isolated in the past; however, our isolate from spinach belonging to the 16SrI group is the first spinach isolate from Iran. DA - 2010 DB - OpenUCT DP - University of Cape Town J1 - Journal of Phytopathology LK - https://open.uct.ac.za PB - University of Cape Town PY - 2010 SM - 0931-1785 T1 - Identification of phytoplasmas associated with cultivated and ornamental plants in Kerman Province, Iran TI - Identification of phytoplasmas associated with cultivated and ornamental plants in Kerman Province, Iran UR - http://hdl.handle.net/11427/20763 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/20763
dc.identifier.urihttp://onlinelibrary.wiley.com/doi/10.1111/j.1439-0434.2010.01682.x/full
dc.identifier.vancouvercitationTazehkand SA, Pour AH, Heydarnejad J, Varsani A, Massumi H. Identification of phytoplasmas associated with cultivated and ornamental plants in Kerman Province, Iran. Journal of Phytopathology. 2010; http://hdl.handle.net/11427/20763.en_ZA
dc.languageengen_ZA
dc.publisherWileyen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.sourceJournal of Phytopathologyen_ZA
dc.source.urihttp://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1439-0434
dc.subject.otherin silico RFLP analysis
dc.subject.otherphytoplasmas
dc.subject.otherspacer region
dc.subject.otherrRNA
dc.subject.otherIran
dc.titleIdentification of phytoplasmas associated with cultivated and ornamental plants in Kerman Province, Iranen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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