An electrophoretic method for the isolation of isohistones from the embryo of the sea urchin Parechinus Angulosus
Master Thesis
1981
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University of Cape Town
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Abstract
It is the aim of this project to isolate sea urchin embryo histone variants by preparative polyacrylamide gel electrophoresis and to prove me identity of the isolated proteins by amino acid composition and partial sequencing. This serves two purposes, namely, the unequivocal identification of chromosomal proteins characterized only by their electrophoretic mobility as histone variants, and secondly the creation of a micropreparative electrophoretic method. These are the prerequisites for later investigations into the specific roles of histones in the process of differentiation.
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Bibliography: leaves 116-122.
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Schwager, S. 1981. An electrophoretic method for the isolation of isohistones from the embryo of the sea urchin Parechinus Angulosus. University of Cape Town.