Omega-3 Fatty Acid and Iron Supplementation Alone, but Not in Combination, Lower Inflammation and Anemia of Infection in Mycobacterium tuberculosis-Infected Mice

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dc.contributor.authorNienaber, Arista
dc.contributor.authorBaumgartner, Jeannine
dc.contributor.authorDolman, Robin C
dc.contributor.authorOzturk, Mumin
dc.contributor.authorZandberg, Lizelle
dc.contributor.authorHayford, Frank E A
dc.contributor.authorBrombacher, Frank
dc.contributor.authorBlaauw, Renee
dc.contributor.authorParihar, Suraj P
dc.contributor.authorSmuts, Cornelius M
dc.contributor.authorMalan, Linda
dc.date.accessioned2021-06-23T13:10:33Z
dc.date.available2021-06-23T13:10:33Z
dc.date.issued2020-09-22
dc.date.updated2020-09-25T13:28:13Z
dc.description.abstractProgressive inflammation and anemia are common in tuberculosis (TB) and linked to poor clinical outcomes. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have inflammation-resolving properties, whereas iron supplementation in TB may have limited efficacy and enhance bacterial growth. We investigated effects of iron and EPA/DHA supplementation, alone and in combination, on inflammation, anemia, iron status markers and clinical outcomes in Mycobacterium tuberculosis-infected C3HeB/FeJ mice. One week post-infection, mice received the AIN-93 diet without (control) or with supplemental iron (Fe), EPA/DHA, or Fe+EPA/DHA for 3 weeks. Mice supplemented with Fe or EPA/DHA had lower soluble transferrin receptor, ferritin and hepcidin than controls, but these effects were attenuated in Fe+EPA/DHA mice. EPA/DHA increased inflammation-resolving lipid mediators and lowered lung IL-1α, IFN-γ, plasma IL-1β, and TNF-α. Fe lowered lung IL-1α, IL-1β, plasma IL-1β, TNF-α, and IL-6. However, the cytokine-lowering effects in the lungs were attenuated with Fe+EPA/DHA. Mice supplemented with EPA/DHA had lower lung bacterial loads than controls, but this effect was attenuated in Fe+EPA/DHA mice. Thus, individually, post-infection EPA/DHA and iron supplementation lowered systemic and lung inflammation and mitigated anemia of infection in TB, but not when combined. EPA/DHA also enhanced bactericidal effects and could support inflammation resolution and management of anemia.
dc.identifierdoi: 10.3390/nu12092897
dc.identifier.apacitationNienaber, A., Baumgartner, J., Dolman, R. C., Ozturk, M., Zandberg, L., Hayford, F. E. A., ... Malan, L. (2020). Omega-3 Fatty Acid and Iron Supplementation Alone, but Not in Combination, Lower Inflammation and Anemia of Infection in Mycobacterium tuberculosis-Infected Mice. <i>Nutrients</i>, 12(9), 2897. http://hdl.handle.net/11427/33386en_ZA
dc.identifier.chicagocitationNienaber, Arista, Jeannine Baumgartner, Robin C Dolman, Mumin Ozturk, Lizelle Zandberg, Frank E A Hayford, Frank Brombacher, et al "Omega-3 Fatty Acid and Iron Supplementation Alone, but Not in Combination, Lower Inflammation and Anemia of Infection in Mycobacterium tuberculosis-Infected Mice." <i>Nutrients</i> 12, 9. (2020): 2897. http://hdl.handle.net/11427/33386en_ZA
dc.identifier.citationNienaber, A., Baumgartner, J., Dolman, R.C., Ozturk, M., Zandberg, L., Hayford, F.E.A., Brombacher, F. & Blaauw, R. et al. 2020. Omega-3 Fatty Acid and Iron Supplementation Alone, but Not in Combination, Lower Inflammation and Anemia of Infection in Mycobacterium tuberculosis-Infected Mice. <i>Nutrients.</i> 12(9):2897. http://hdl.handle.net/11427/33386en_ZA
dc.identifier.risTY - Journal Article AU - Nienaber, Arista AU - Baumgartner, Jeannine AU - Dolman, Robin C AU - Ozturk, Mumin AU - Zandberg, Lizelle AU - Hayford, Frank E A AU - Brombacher, Frank AU - Blaauw, Renee AU - Parihar, Suraj P AU - Smuts, Cornelius M AU - Malan, Linda AB - Progressive inflammation and anemia are common in tuberculosis (TB) and linked to poor clinical outcomes. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have inflammation-resolving properties, whereas iron supplementation in TB may have limited efficacy and enhance bacterial growth. We investigated effects of iron and EPA/DHA supplementation, alone and in combination, on inflammation, anemia, iron status markers and clinical outcomes in <i>Mycobacterium tuberculosis</i>-infected C3HeB/FeJ mice. One week post-infection, mice received the AIN-93 diet without (control) or with supplemental iron (Fe), EPA/DHA, or Fe+EPA/DHA for 3 weeks. Mice supplemented with Fe or EPA/DHA had lower soluble transferrin receptor, ferritin and hepcidin than controls, but these effects were attenuated in Fe+EPA/DHA mice. EPA/DHA increased inflammation-resolving lipid mediators and lowered lung IL-1&alpha;, IFN-&gamma;, plasma IL-1&beta;, and TNF-&alpha;. Fe lowered lung IL-1&alpha;, IL-1&beta;, plasma IL-1&beta;, TNF-&alpha;, and IL-6. However, the cytokine-lowering effects in the lungs were attenuated with Fe+EPA/DHA. Mice supplemented with EPA/DHA had lower lung bacterial loads than controls, but this effect was attenuated in Fe+EPA/DHA mice. Thus, individually, post-infection EPA/DHA and iron supplementation lowered systemic and lung inflammation and mitigated anemia of infection in TB, but not when combined. EPA/DHA also enhanced bactericidal effects and could support inflammation resolution and management of anemia. DA - 2020-09-22 DB - OpenUCT DP - University of Cape Town IS - 9 J1 - Nutrients LK - https://open.uct.ac.za PY - 2020 T1 - Omega-3 Fatty Acid and Iron Supplementation Alone, but Not in Combination, Lower Inflammation and Anemia of Infection in Mycobacterium tuberculosis-Infected Mice TI - Omega-3 Fatty Acid and Iron Supplementation Alone, but Not in Combination, Lower Inflammation and Anemia of Infection in Mycobacterium tuberculosis-Infected Mice UR - http://hdl.handle.net/11427/33386 ER -en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/33386
dc.identifier.vancouvercitationNienaber A, Baumgartner J, Dolman RC, Ozturk M, Zandberg L, Hayford FEA, et al. Omega-3 Fatty Acid and Iron Supplementation Alone, but Not in Combination, Lower Inflammation and Anemia of Infection in Mycobacterium tuberculosis-Infected Mice. Nutrients. 2020;12(9):2897. http://hdl.handle.net/11427/33386.en_ZA
dc.sourceNutrients
dc.source.journalissue9
dc.source.journalvolume12
dc.source.pagination2897
dc.source.urihttps://www.mdpi.com/journal/nutrients
dc.titleOmega-3 Fatty Acid and Iron Supplementation Alone, but Not in Combination, Lower Inflammation and Anemia of Infection in Mycobacterium tuberculosis-Infected Mice
dc.typeJournal Article
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