The biological properties of three trichothecene mycotoxins produces by fusaris

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dc.contributor.advisorThiel, Pieter Gen_ZA
dc.contributor.advisorIvanetich, Kathryn Men_ZA
dc.contributor.authorJanse Van Rensburg, Daniel Francoisen_ZA
dc.date.accessioned2018-02-01T13:32:52Z
dc.date.available2018-02-01T13:32:52Z
dc.date.issued1986en_ZA
dc.description.abstractThe highly toxic fungal metabolite, neosolaniol monoacetate, was isolated and purified from cultures of Fusarium sambucinum. Since little is known about its toxic properties, the biological effects of this trichothecene were compared to those caused by diacetoxy-scirpenol in male Wistar rats. The lesions caused by the two toxins were very similar. Chronic exposure to either toxin led to a significant decrease (P<0.05) in red blood cell counts and a significant increase (P<0.05) in platelet size. The major pathological lesions observed were atrophy of the actively dividing cells of the bone marrow, thymus, spleen and lymph nodes. The reported species difference in T-2 toxin toxicity was investigated by determining the deacylation rate of T-2 toxin to HT-2 toxin, one of the first steps in the detoxification of this trichothecene. The high deacylation rate catalysed by rat microsomes correlated with the low sensitivity of this species to T-2 toxin, whereas the low deacylation rates with cat and monkey microsomes agreed with their high sensitivity. In contrast to this, the apparently high toxicity of T-2 toxin to humans does not correlate with the high deacylation rate observed in human hepatic microsomes. Involvement of the UDP-glucuronyltransferases in the detoxification of T-2 toxin was studied with rat and pig hepatic microsomes. T-2 toxin and two of its metabolites, HT-2 toxin and T-2 tetraol, did not appear to act as substrates for these enzymes under the in vitro conditions used.en_ZA
dc.identifier.apacitationJanse Van Rensburg, D. F. (1986). <i>The biological properties of three trichothecene mycotoxins produces by fusaris</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry & Structural Biology. Retrieved from http://hdl.handle.net/11427/27209en_ZA
dc.identifier.chicagocitationJanse Van Rensburg, Daniel Francois. <i>"The biological properties of three trichothecene mycotoxins produces by fusaris."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry & Structural Biology, 1986. http://hdl.handle.net/11427/27209en_ZA
dc.identifier.citationJanse Van Rensburg, D. 1986. The biological properties of three trichothecene mycotoxins produces by fusaris. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Janse Van Rensburg, Daniel Francois AB - The highly toxic fungal metabolite, neosolaniol monoacetate, was isolated and purified from cultures of Fusarium sambucinum. Since little is known about its toxic properties, the biological effects of this trichothecene were compared to those caused by diacetoxy-scirpenol in male Wistar rats. The lesions caused by the two toxins were very similar. Chronic exposure to either toxin led to a significant decrease (P<0.05) in red blood cell counts and a significant increase (P<0.05) in platelet size. The major pathological lesions observed were atrophy of the actively dividing cells of the bone marrow, thymus, spleen and lymph nodes. The reported species difference in T-2 toxin toxicity was investigated by determining the deacylation rate of T-2 toxin to HT-2 toxin, one of the first steps in the detoxification of this trichothecene. The high deacylation rate catalysed by rat microsomes correlated with the low sensitivity of this species to T-2 toxin, whereas the low deacylation rates with cat and monkey microsomes agreed with their high sensitivity. In contrast to this, the apparently high toxicity of T-2 toxin to humans does not correlate with the high deacylation rate observed in human hepatic microsomes. Involvement of the UDP-glucuronyltransferases in the detoxification of T-2 toxin was studied with rat and pig hepatic microsomes. T-2 toxin and two of its metabolites, HT-2 toxin and T-2 tetraol, did not appear to act as substrates for these enzymes under the in vitro conditions used. DA - 1986 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1986 T1 - The biological properties of three trichothecene mycotoxins produces by fusaris TI - The biological properties of three trichothecene mycotoxins produces by fusaris UR - http://hdl.handle.net/11427/27209 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/27209
dc.identifier.vancouvercitationJanse Van Rensburg DF. The biological properties of three trichothecene mycotoxins produces by fusaris. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry & Structural Biology, 1986 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/27209en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Medical Biochemistry and Structural Biology
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMycotoxinsen_ZA
dc.subject.otherSesquiterpenesen_ZA
dc.subject.otherFusariumen_ZA
dc.titleThe biological properties of three trichothecene mycotoxins produces by fusarisen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMSc (Med)en_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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