An investigation of Epstein-Barr Virus (EBV) latency type and MYC gene aberrations in plasmablastic lymphoma diagnosed at Groote Schuur Hospital, Cape Town, South Africa

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dc.contributor.advisorRamburan, Amsha
dc.contributor.advisorGovender, Dhirendra
dc.contributor.authorKriel, Raymond Frank
dc.date.accessioned2021-01-27T14:27:51Z
dc.date.available2021-01-27T14:27:51Z
dc.date.issued2020
dc.date.updated2021-01-27T14:27:02Z
dc.description.abstractIntroduction: Plasmablastic lymphoma (PBL) is a rare, aggressive, AIDS-associated non-Hodgkin lymphoma. The pathogenesis of PBL is incompletely understood, however association with the Epstein-Barr virus (EBV) and the MYC gene, have been identified as important pathogenic mechanisms. Aims and objectives: To characterise the EBV latency in a cohort of patients diagnosed with PBL at Groote Schuur Hospital (GSH), by means of immunohistochemistry. To determine MYC gene aberrations using fluorescent in situ hybridisation (FISH). Materials and methods: The cohort comprised PBL cases diagnosed from 2005-2017. EBER ISH was used to confirm EBV infection. Manual immunohistochemistry using three monoclonal antibodies for EBV latent proteins, (EBNA1, EBNA2 and LMP1) was used to determine the latency type. Manual MYC FISH was performed on all PBL cases using a dual colour break apart rearrangement probe. Results: Forty-nine cases of PBL were included in this study. Forty-one cases were positive for EBER ISH. Thirty-seven (78.7%) cases showed HIV/EBV coinfection. Latency 0 was observed in 29 (70.7%) cases, latency 1 in 8 (19.5%) and latency 2 in 4 (9.8%) cases. MYC FISH was performed on all 49 PBL cases, of which 30 (61.2%) yielded a result. MYC was intact in 11 (36.7%), translocated in 8 (26.7%) and 11 (36.7 %) cases showed copy number variations. Conclusion: Our research demonstrated 37 (90.2%) of the EBV positive PBL cases showed a restricted latency pattern of 0 or 1. Furthermore we found that MYC gene aberrations consisting of translocations and copy number variations occurred in 19 cases (63.3%) , with copy number variations being higher than cited in current literature. Our study is also the first to investigate PBL EBV latency in SA. An uncommon finding was the existence of MYC gene aberrations in HIV positive, EBV negative PBL cases.
dc.identifier.apacitationKriel, R. F. (2020). <i>An investigation of Epstein-Barr Virus (EBV) latency type and MYC gene aberrations in plasmablastic lymphoma diagnosed at Groote Schuur Hospital, Cape Town, South Africa</i>. (). ,Faculty of Health Sciences ,Department of Clinical Laboratory Sciences. Retrieved from http://hdl.handle.net/11427/32714en_ZA
dc.identifier.chicagocitationKriel, Raymond Frank. <i>"An investigation of Epstein-Barr Virus (EBV) latency type and MYC gene aberrations in plasmablastic lymphoma diagnosed at Groote Schuur Hospital, Cape Town, South Africa."</i> ., ,Faculty of Health Sciences ,Department of Clinical Laboratory Sciences, 2020. http://hdl.handle.net/11427/32714en_ZA
dc.identifier.citationKriel, R.F. 2020. An investigation of Epstein-Barr Virus (EBV) latency type and MYC gene aberrations in plasmablastic lymphoma diagnosed at Groote Schuur Hospital, Cape Town, South Africa. . ,Faculty of Health Sciences ,Department of Clinical Laboratory Sciences. http://hdl.handle.net/11427/32714en_ZA
dc.identifier.ris TY - Master Thesis AU - Kriel, Raymond Frank AB - Introduction: Plasmablastic lymphoma (PBL) is a rare, aggressive, AIDS-associated non-Hodgkin lymphoma. The pathogenesis of PBL is incompletely understood, however association with the Epstein-Barr virus (EBV) and the MYC gene, have been identified as important pathogenic mechanisms. Aims and objectives: To characterise the EBV latency in a cohort of patients diagnosed with PBL at Groote Schuur Hospital (GSH), by means of immunohistochemistry. To determine MYC gene aberrations using fluorescent in situ hybridisation (FISH). Materials and methods: The cohort comprised PBL cases diagnosed from 2005-2017. EBER ISH was used to confirm EBV infection. Manual immunohistochemistry using three monoclonal antibodies for EBV latent proteins, (EBNA1, EBNA2 and LMP1) was used to determine the latency type. Manual MYC FISH was performed on all PBL cases using a dual colour break apart rearrangement probe. Results: Forty-nine cases of PBL were included in this study. Forty-one cases were positive for EBER ISH. Thirty-seven (78.7%) cases showed HIV/EBV coinfection. Latency 0 was observed in 29 (70.7%) cases, latency 1 in 8 (19.5%) and latency 2 in 4 (9.8%) cases. MYC FISH was performed on all 49 PBL cases, of which 30 (61.2%) yielded a result. MYC was intact in 11 (36.7%), translocated in 8 (26.7%) and 11 (36.7 %) cases showed copy number variations. Conclusion: Our research demonstrated 37 (90.2%) of the EBV positive PBL cases showed a restricted latency pattern of 0 or 1. Furthermore we found that MYC gene aberrations consisting of translocations and copy number variations occurred in 19 cases (63.3%) , with copy number variations being higher than cited in current literature. Our study is also the first to investigate PBL EBV latency in SA. An uncommon finding was the existence of MYC gene aberrations in HIV positive, EBV negative PBL cases. DA - 2020 DB - OpenUCT DP - University of Cape Town KW - Medicine LK - https://open.uct.ac.za PY - 2020 T1 - An investigation of Epstein-Barr Virus (EBV) latency type and MYC gene aberrations in plasmablastic lymphoma diagnosed at Groote Schuur Hospital, Cape Town, South Africa TI - An investigation of Epstein-Barr Virus (EBV) latency type and MYC gene aberrations in plasmablastic lymphoma diagnosed at Groote Schuur Hospital, Cape Town, South Africa UR - http://hdl.handle.net/11427/32714 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/32714
dc.identifier.vancouvercitationKriel RF. An investigation of Epstein-Barr Virus (EBV) latency type and MYC gene aberrations in plasmablastic lymphoma diagnosed at Groote Schuur Hospital, Cape Town, South Africa. []. ,Faculty of Health Sciences ,Department of Clinical Laboratory Sciences, 2020 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/32714en_ZA
dc.language.rfc3066eng
dc.publisher.departmentDepartment of Clinical Laboratory Sciences
dc.publisher.facultyFaculty of Health Sciences
dc.subjectMedicine
dc.titleAn investigation of Epstein-Barr Virus (EBV) latency type and MYC gene aberrations in plasmablastic lymphoma diagnosed at Groote Schuur Hospital, Cape Town, South Africa
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationlevelMSc
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