Parallel changes in gene expression in peripheral blood mononuclear cells and the brain after maternal separation in the mouse

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dc.contributor.authorvan Heerden, Johanen_ZA
dc.contributor.authorConesa, Anaen_ZA
dc.contributor.authorStein, Dan Jen_ZA
dc.contributor.authorMontaner, Daviden_ZA
dc.contributor.authorRussell, Vivienneen_ZA
dc.contributor.authorIlling, Nicolaen_ZA
dc.date.accessioned2015-11-04T11:57:26Z
dc.date.available2015-11-04T11:57:26Z
dc.date.issued2009en_ZA
dc.description.abstractBACKGROUND: The functional integration of the neuro-, endocrine- and immune-systems suggests that the transcriptome of white blood cells may reflect neuropsychiatric states, and be used as a non-invasive diagnostic indicator. We used a mouse maternal separation model, a paradigm of early adversity, to test the hypothesis that transcriptional changes in peripheral blood mononuclear cells (PBMCs) are paralleled by specific gene expression changes in prefrontal cortex (PFC), hippocampus (Hic) and hypothalamus (Hyp). Furthermore, we evaluated whether gene expression profiles of PBMCs could be used to predict the separation status of individual animals.FINDINGS:Microarray gene expression profiles of all three brain regions provided substantial evidence of stress-related neural differences between maternally separated and control animals. For example, changes in expression of genes involved in the glutamatergic and GABAergic systems were identified in the PFC and Hic, supporting a stress-related hyperglutamatergic state within the separated group. The expression of 50 genes selected from the PBMC microarray data provided sufficient information to predict treatment classes with 95% accuracy. Importantly, stress-related transcriptome differences in PBMC populations were paralleled by stress-related gene expression changes in CNS target tissues. CONCLUSION: These results confirm that the transcriptional profiles of peripheral immune tissues occur in parallel to changes in the brain and contain sufficient information for the efficient diagnostic prediction of stress-related neural states in mice. Future studies will need to evaluate the relevance of the predictor set of 50 genes within clinical settings, specifically within a context of stress-related disorders.en_ZA
dc.identifier.apacitationvan Heerden, J., Conesa, A., Stein, D. J., Montaner, D., Russell, V., & Illing, N. (2009). Parallel changes in gene expression in peripheral blood mononuclear cells and the brain after maternal separation in the mouse. <i>BMC Research Notes</i>, http://hdl.handle.net/11427/14682en_ZA
dc.identifier.chicagocitationvan Heerden, Johan, Ana Conesa, Dan J Stein, David Montaner, Vivienne Russell, and Nicola Illing "Parallel changes in gene expression in peripheral blood mononuclear cells and the brain after maternal separation in the mouse." <i>BMC Research Notes</i> (2009) http://hdl.handle.net/11427/14682en_ZA
dc.identifier.citationvan Heerden, J. H., Conesa, A., Stein, D. J., Montaner, D., Russell, V., & Illing, N. (2009). Parallel changes in gene expression in peripheral blood mononuclear cells and the brain after maternal separation in the mouse. BMC research notes, 2(1), 195.en_ZA
dc.identifier.ris TY - Journal Article AU - van Heerden, Johan AU - Conesa, Ana AU - Stein, Dan J AU - Montaner, David AU - Russell, Vivienne AU - Illing, Nicola AB - BACKGROUND: The functional integration of the neuro-, endocrine- and immune-systems suggests that the transcriptome of white blood cells may reflect neuropsychiatric states, and be used as a non-invasive diagnostic indicator. We used a mouse maternal separation model, a paradigm of early adversity, to test the hypothesis that transcriptional changes in peripheral blood mononuclear cells (PBMCs) are paralleled by specific gene expression changes in prefrontal cortex (PFC), hippocampus (Hic) and hypothalamus (Hyp). Furthermore, we evaluated whether gene expression profiles of PBMCs could be used to predict the separation status of individual animals.FINDINGS:Microarray gene expression profiles of all three brain regions provided substantial evidence of stress-related neural differences between maternally separated and control animals. For example, changes in expression of genes involved in the glutamatergic and GABAergic systems were identified in the PFC and Hic, supporting a stress-related hyperglutamatergic state within the separated group. The expression of 50 genes selected from the PBMC microarray data provided sufficient information to predict treatment classes with 95% accuracy. Importantly, stress-related transcriptome differences in PBMC populations were paralleled by stress-related gene expression changes in CNS target tissues. CONCLUSION: These results confirm that the transcriptional profiles of peripheral immune tissues occur in parallel to changes in the brain and contain sufficient information for the efficient diagnostic prediction of stress-related neural states in mice. Future studies will need to evaluate the relevance of the predictor set of 50 genes within clinical settings, specifically within a context of stress-related disorders. DA - 2009 DB - OpenUCT DO - 10.1186/1756-0500-2-195 DP - University of Cape Town J1 - BMC Research Notes LK - https://open.uct.ac.za PB - University of Cape Town PY - 2009 T1 - Parallel changes in gene expression in peripheral blood mononuclear cells and the brain after maternal separation in the mouse TI - Parallel changes in gene expression in peripheral blood mononuclear cells and the brain after maternal separation in the mouse UR - http://hdl.handle.net/11427/14682 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/14682
dc.identifier.urihttp://dx.doi.org/10.1186/1756-0500-2-195
dc.identifier.vancouvercitationvan Heerden J, Conesa A, Stein DJ, Montaner D, Russell V, Illing N. Parallel changes in gene expression in peripheral blood mononuclear cells and the brain after maternal separation in the mouse. BMC Research Notes. 2009; http://hdl.handle.net/11427/14682.en_ZA
dc.language.isoengen_ZA
dc.publisherBioMed Central Ltden_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution Licenseen_ZA
dc.rights.holder2009 Illing et al; licensee BioMed Central Ltd.en_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en_ZA
dc.sourceBMC Research Notesen_ZA
dc.source.urihttp://www.biomedcentral.com/bmcresnotes/en_ZA
dc.subject.otherPBMC Gene Expressionen_ZA
dc.subject.otherMS Mouseen_ZA
dc.subject.otherPBMC Gene Expression Profileen_ZA
dc.subject.otherSh Sampleen_ZA
dc.subject.otherGene Set Enrichment Analysisen_ZA
dc.subject.otherAcute Restraint Stressen_ZA
dc.subject.otherMaternal Separationen_ZA
dc.subject.otherDE Geneen_ZA
dc.subject.otherSupport Vector Machineen_ZA
dc.titleParallel changes in gene expression in peripheral blood mononuclear cells and the brain after maternal separation in the mouseen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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