Characterisation of the T cell responses induced by BCG in infants over the first year of life

Simple item page
dc.contributor.advisorHanekom, Willem Aen_ZA
dc.contributor.authorKwong Chung, Cheong Kwet Choyen_ZA
dc.date.accessioned2015-07-14T08:51:40Z
dc.date.available2015-07-14T08:51:40Z
dc.date.issued2011en_ZA
dc.descriptionIncludes bibiliographical references.en_ZA
dc.description.abstractMycobacterium bovis Bacille Calmette Guerin (BCG) is the only licensed tuberculosis (TB) vaccine. Despite the immunisation of 3 billion individuals with this vaccine, TB remains a major cause of mortality worldwide. Therefore, there is an urgent need for more effective TB vaccines. BCG is likely to remain central to future TB prevention strategies, which could include a BCG prime at birth, followed by boosts with novel TB vaccines within the first year of life, or at later ages. Therefore, a comprehensive understanding of BCG induced immunity is required for the successful design and implementation of novel TB vaccination strategies. This was addressed in the following two studies.The aim of the first study was to characterise specific T cell immunity following BCG vaccination. These data are critical to determine when to optimally boost BCG induced immunity in infants. We enrolled infants routinely vaccinated with BCG at birth, and determined the frequency of T cells induced by immunisation, at various time points over the first year of life. The T cells were identified by binding of cell surface markers and characterised by cell-specific cytokine production, following 12 hr incubation of infant whole blood with BCG. Multiparameter flow cytometry was used for the analysis. We found that the peak vaccine induced CD4+ T cell response occurred at 10 weeks, followed by a contraction phase. BCG specific CD4+ T cells became more poly functional, and acquired the profile of long-lived T cells (measured by Bcl-2 expression), over the first year of life.en_ZA
dc.identifier.apacitationKwong Chung, C. K. C. (2011). <i>Characterisation of the T cell responses induced by BCG in infants over the first year of life</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Department of Paediatrics and Child Health. Retrieved from http://hdl.handle.net/11427/13439en_ZA
dc.identifier.chicagocitationKwong Chung, Cheong Kwet Choy. <i>"Characterisation of the T cell responses induced by BCG in infants over the first year of life."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Department of Paediatrics and Child Health, 2011. http://hdl.handle.net/11427/13439en_ZA
dc.identifier.citationKwong Chung, C. 2011. Characterisation of the T cell responses induced by BCG in infants over the first year of life. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Kwong Chung, Cheong Kwet Choy AB - Mycobacterium bovis Bacille Calmette Guerin (BCG) is the only licensed tuberculosis (TB) vaccine. Despite the immunisation of 3 billion individuals with this vaccine, TB remains a major cause of mortality worldwide. Therefore, there is an urgent need for more effective TB vaccines. BCG is likely to remain central to future TB prevention strategies, which could include a BCG prime at birth, followed by boosts with novel TB vaccines within the first year of life, or at later ages. Therefore, a comprehensive understanding of BCG induced immunity is required for the successful design and implementation of novel TB vaccination strategies. This was addressed in the following two studies.The aim of the first study was to characterise specific T cell immunity following BCG vaccination. These data are critical to determine when to optimally boost BCG induced immunity in infants. We enrolled infants routinely vaccinated with BCG at birth, and determined the frequency of T cells induced by immunisation, at various time points over the first year of life. The T cells were identified by binding of cell surface markers and characterised by cell-specific cytokine production, following 12 hr incubation of infant whole blood with BCG. Multiparameter flow cytometry was used for the analysis. We found that the peak vaccine induced CD4+ T cell response occurred at 10 weeks, followed by a contraction phase. BCG specific CD4+ T cells became more poly functional, and acquired the profile of long-lived T cells (measured by Bcl-2 expression), over the first year of life. DA - 2011 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2011 T1 - Characterisation of the T cell responses induced by BCG in infants over the first year of life TI - Characterisation of the T cell responses induced by BCG in infants over the first year of life UR - http://hdl.handle.net/11427/13439 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/13439
dc.identifier.vancouvercitationKwong Chung CKC. Characterisation of the T cell responses induced by BCG in infants over the first year of life. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Department of Paediatrics and Child Health, 2011 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/13439en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Paediatrics and Child Healthen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherPaediatrics and Child Healthen_ZA
dc.titleCharacterisation of the T cell responses induced by BCG in infants over the first year of lifeen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMSc (Med)en_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
thesis_hsf_2011_kwong_chung_c.pdf
Size:
2.52 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Masters