Differences in genotype and virulence among four multidrug-resistant Streptococcus pneumoniae isolates belonging to the PMEN1 clone

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dc.contributor.authorHiller, N Luisaen_ZA
dc.contributor.authorEutsey, Rory Aen_ZA
dc.contributor.authorPowell, Evanen_ZA
dc.contributor.authorEarl, Joshua Pen_ZA
dc.contributor.authorJanto, Benjaminen_ZA
dc.contributor.authorMartin, Darren Pen_ZA
dc.contributor.authorDawid, Suzanneen_ZA
dc.contributor.authorAhmed, Azaden_ZA
dc.contributor.authorLongwell, Mark Jen_ZA
dc.contributor.authorDahlgren, Margaret Een_ZA
dc.date.accessioned2016-01-11T06:53:20Z
dc.date.available2016-01-11T06:53:20Z
dc.date.issued2011en_ZA
dc.description.abstractWe report on the comparative genomics and characterization of the virulence phenotypes of four S. pneumoniae strains that belong to the multidrug resistant clone PMEN1 (Spain 23F ST81). Strains SV35-T23 and SV36-T3 were recovered in 1996 from the nasopharynx of patients at an AIDS hospice in New York. Strain SV36-T3 expressed capsule type 3 which is unusual for this clone and represents the product of an in vivo capsular switch event. A third PMEN1 isolate - PN4595-T23 - was recovered in 1996 from the nasopharynx of a child attending day care in Portugal, and a fourth strain - ATCC700669 - was originally isolated from a patient with pneumococcal disease in Spain in 1984. We compared the genomes among four PMEN1 strains and 47 previously sequenced pneumococcal isolates for gene possession differences and allelic variations within core genes. In contrast to the 47 strains - representing a variety of clonal types - the four PMEN1 strains grouped closely together, demonstrating high genomic conservation within this lineage relative to the rest of the species. In the four PMEN1 strains allelic and gene possession differences were clustered into 18 genomic regions including the capsule, the blp bacteriocins, erythromycin resistance, the MM1-2008 prophage and multiple cell wall anchored proteins. In spite of their genomic similarity, the high resolution chinchilla model was able to detect variations in virulence properties of the PMEN1 strains highlighting how small genic or allelic variation can lead to significant changes in pathogenicity and making this set of strains ideal for the identification of novel virulence determinants.en_ZA
dc.identifier.apacitationHiller, N. L., Eutsey, R. A., Powell, E., Earl, J. P., Janto, B., Martin, D. P., ... Dahlgren, M. E. (2011). Differences in genotype and virulence among four multidrug-resistant Streptococcus pneumoniae isolates belonging to the PMEN1 clone. <i>PLoS One</i>, http://hdl.handle.net/11427/16269en_ZA
dc.identifier.chicagocitationHiller, N Luisa, Rory A Eutsey, Evan Powell, Joshua P Earl, Benjamin Janto, Darren P Martin, Suzanne Dawid, Azad Ahmed, Mark J Longwell, and Margaret E Dahlgren "Differences in genotype and virulence among four multidrug-resistant Streptococcus pneumoniae isolates belonging to the PMEN1 clone." <i>PLoS One</i> (2011) http://hdl.handle.net/11427/16269en_ZA
dc.identifier.citationHiller, N. L., Eutsey, R. A., Powell, E., Earl, J. P., Janto, B., Martin, D. P., ... & Ezzo, S. (2011). Differences in genotype and virulence among four multidrug-resistant Streptococcus pneumoniae isolates belonging to the PMEN1 clone. PloS one, 6(12), 28850. doi:10.1371/journal.pone.0028850en_ZA
dc.identifier.ris TY - Journal Article AU - Hiller, N Luisa AU - Eutsey, Rory A AU - Powell, Evan AU - Earl, Joshua P AU - Janto, Benjamin AU - Martin, Darren P AU - Dawid, Suzanne AU - Ahmed, Azad AU - Longwell, Mark J AU - Dahlgren, Margaret E AB - We report on the comparative genomics and characterization of the virulence phenotypes of four S. pneumoniae strains that belong to the multidrug resistant clone PMEN1 (Spain 23F ST81). Strains SV35-T23 and SV36-T3 were recovered in 1996 from the nasopharynx of patients at an AIDS hospice in New York. Strain SV36-T3 expressed capsule type 3 which is unusual for this clone and represents the product of an in vivo capsular switch event. A third PMEN1 isolate - PN4595-T23 - was recovered in 1996 from the nasopharynx of a child attending day care in Portugal, and a fourth strain - ATCC700669 - was originally isolated from a patient with pneumococcal disease in Spain in 1984. We compared the genomes among four PMEN1 strains and 47 previously sequenced pneumococcal isolates for gene possession differences and allelic variations within core genes. In contrast to the 47 strains - representing a variety of clonal types - the four PMEN1 strains grouped closely together, demonstrating high genomic conservation within this lineage relative to the rest of the species. In the four PMEN1 strains allelic and gene possession differences were clustered into 18 genomic regions including the capsule, the blp bacteriocins, erythromycin resistance, the MM1-2008 prophage and multiple cell wall anchored proteins. In spite of their genomic similarity, the high resolution chinchilla model was able to detect variations in virulence properties of the PMEN1 strains highlighting how small genic or allelic variation can lead to significant changes in pathogenicity and making this set of strains ideal for the identification of novel virulence determinants. DA - 2011 DB - OpenUCT DO - 10.1371/journal.pone.0028850 DP - University of Cape Town J1 - PLoS One LK - https://open.uct.ac.za PB - University of Cape Town PY - 2011 T1 - Differences in genotype and virulence among four multidrug-resistant Streptococcus pneumoniae isolates belonging to the PMEN1 clone TI - Differences in genotype and virulence among four multidrug-resistant Streptococcus pneumoniae isolates belonging to the PMEN1 clone UR - http://hdl.handle.net/11427/16269 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/16269
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pone.0028850
dc.identifier.vancouvercitationHiller NL, Eutsey RA, Powell E, Earl JP, Janto B, Martin DP, et al. Differences in genotype and virulence among four multidrug-resistant Streptococcus pneumoniae isolates belonging to the PMEN1 clone. PLoS One. 2011; http://hdl.handle.net/11427/16269.en_ZA
dc.language.isoengen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.publisher.departmentInstitute of Infectious Disease and Molecular Medicineen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_ZA
dc.rights.holder© 2011 Hiller et alen_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/4.0en_ZA
dc.sourcePLoS Oneen_ZA
dc.source.urihttp://journals.plos.org/plosoneen_ZA
dc.subject.otherBacteriocinsen_ZA
dc.subject.otherChinchillasen_ZA
dc.subject.otherGenomic databasesen_ZA
dc.subject.otherImmunityen_ZA
dc.subject.otherSequence alignmenten_ZA
dc.subject.otherOtologyen_ZA
dc.subject.otherSequence assembly toolsen_ZA
dc.subject.otherCloningen_ZA
dc.titleDifferences in genotype and virulence among four multidrug-resistant Streptococcus pneumoniae isolates belonging to the PMEN1 cloneen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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