Attachment of Acidithiobacillus ferrooxidans and Leptospirillum ferriphilum cultured under varying conditions to pyrite, chalcopyrite, low-grade ore and quartz in a packed column reactor

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dc.contributor.authorAfrica, Cindy-Jade
dc.contributor.authorvan Hille, Robert P
dc.contributor.authorHarrison, Susan T L
dc.date.accessioned2016-08-17T11:09:48Z
dc.date.available2016-08-17T11:09:48Z
dc.date.issued2012
dc.date.updated2016-08-17T11:08:20Z
dc.description.abstractThe attachment of Acidithiobacillus ferrooxidans and Leptospirillum ferriphilum spp. grown on ferrous medium or adapted to a pyrite mineral concentrate to four mineral substrata, namely, chalcopyrite and pyrite concentrates, a low-grade chalcopyrite ore (0.5 wt%) and quartzite, was investigated. The quartzite represented a typical gangue mineral and served as a control. The attachment studies were carried out in a novel particle-coated column reactor. The saturated reactor containing glass beads, which were coated with fine mineral concentrates, provided a quantifiable surface area of mineral concentrate and maintained good fluid flow. A. ferrooxidans and Leptospirillum spp. had similar attachment characteristics. Enhanced attachment efficiency occurred with bacteria grown on sulphide minerals relative to those grown on ferrous sulphate in an ore-free environment. Selective attachment to sulphide minerals relative to gangue materials occurred, with mineral adapted cultures attaching to the minerals more efficiently than ferrous grown cultures. Mineral-adapted cultures showed highest levels of attachment to pyrite (74% and 79% attachment for A. ferrooxidans and L. ferriphilum, respectively). This was followed by attachment of mineral-adapted cultures to chalcopyrite (63% and 58% for A. ferrooxidans and L. ferriphilum, respectively). A. ferrooxidans and L. ferriphilum exhibited lower levels of attachment to low-grade ore and quartz relative to the sulphide minerals.en_ZA
dc.identifierhttp://dx.doi.org/10.1007/s00253-012-3939-x
dc.identifier.apacitationAfrica, C., van Hille, R. P., & Harrison, S. T. L. (2012). Attachment of Acidithiobacillus ferrooxidans and Leptospirillum ferriphilum cultured under varying conditions to pyrite, chalcopyrite, low-grade ore and quartz in a packed column reactor. <i>Applied Microbiology and Biotechnology</i>, http://hdl.handle.net/11427/21301en_ZA
dc.identifier.chicagocitationAfrica, Cindy-Jade, Robert P van Hille, and Susan T L Harrison "Attachment of Acidithiobacillus ferrooxidans and Leptospirillum ferriphilum cultured under varying conditions to pyrite, chalcopyrite, low-grade ore and quartz in a packed column reactor." <i>Applied Microbiology and Biotechnology</i> (2012) http://hdl.handle.net/11427/21301en_ZA
dc.identifier.citationAfrica, C. J., van Hille, R. P., & Harrison, S. T. (2013). Attachment of Acidithiobacillus ferrooxidans and Leptospirillum ferriphilum cultured under varying conditions to pyrite, chalcopyrite, low-grade ore and quartz in a packed column reactor. Applied microbiology and biotechnology, 97(3), 1317-1324.en_ZA
dc.identifier.issn0175-7598en_ZA
dc.identifier.ris TY - Journal Article AU - Africa, Cindy-Jade AU - van Hille, Robert P AU - Harrison, Susan T L AB - The attachment of Acidithiobacillus ferrooxidans and Leptospirillum ferriphilum spp. grown on ferrous medium or adapted to a pyrite mineral concentrate to four mineral substrata, namely, chalcopyrite and pyrite concentrates, a low-grade chalcopyrite ore (0.5 wt%) and quartzite, was investigated. The quartzite represented a typical gangue mineral and served as a control. The attachment studies were carried out in a novel particle-coated column reactor. The saturated reactor containing glass beads, which were coated with fine mineral concentrates, provided a quantifiable surface area of mineral concentrate and maintained good fluid flow. A. ferrooxidans and Leptospirillum spp. had similar attachment characteristics. Enhanced attachment efficiency occurred with bacteria grown on sulphide minerals relative to those grown on ferrous sulphate in an ore-free environment. Selective attachment to sulphide minerals relative to gangue materials occurred, with mineral adapted cultures attaching to the minerals more efficiently than ferrous grown cultures. Mineral-adapted cultures showed highest levels of attachment to pyrite (74% and 79% attachment for A. ferrooxidans and L. ferriphilum, respectively). This was followed by attachment of mineral-adapted cultures to chalcopyrite (63% and 58% for A. ferrooxidans and L. ferriphilum, respectively). A. ferrooxidans and L. ferriphilum exhibited lower levels of attachment to low-grade ore and quartz relative to the sulphide minerals. DA - 2012 DB - OpenUCT DP - University of Cape Town J1 - Applied Microbiology and Biotechnology LK - https://open.uct.ac.za PB - University of Cape Town PY - 2012 SM - 0175-7598 T1 - Attachment of Acidithiobacillus ferrooxidans and Leptospirillum ferriphilum cultured under varying conditions to pyrite, chalcopyrite, low-grade ore and quartz in a packed column reactor TI - Attachment of Acidithiobacillus ferrooxidans and Leptospirillum ferriphilum cultured under varying conditions to pyrite, chalcopyrite, low-grade ore and quartz in a packed column reactor UR - http://hdl.handle.net/11427/21301 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/21301
dc.identifier.urihttp://link.springer.com/article/10.1007/s00253-012-3939-x
dc.identifier.vancouvercitationAfrica C, van Hille RP, Harrison STL. Attachment of Acidithiobacillus ferrooxidans and Leptospirillum ferriphilum cultured under varying conditions to pyrite, chalcopyrite, low-grade ore and quartz in a packed column reactor. Applied Microbiology and Biotechnology. 2012; http://hdl.handle.net/11427/21301.en_ZA
dc.languageengen_ZA
dc.publisherSpringer Verlagen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.sourceApplied Microbiology and Biotechnologyen_ZA
dc.source.urihttp://link.springer.com/journal/253
dc.subject.otherBioleaching
dc.subject.otherLow-grade chalcopyrite
dc.subject.otherPyrite
dc.subject.otherLeptospirillum ferriphilum
dc.subject.otherPyrite
dc.titleAttachment of Acidithiobacillus ferrooxidans and Leptospirillum ferriphilum cultured under varying conditions to pyrite, chalcopyrite, low-grade ore and quartz in a packed column reactoren_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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