Process integration in the optimisation of amidase production from recombinant Escherichia coli

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dc.contributor.advisorHarrison, STLen_ZA
dc.contributor.authorOlaofe, Oluwafemi Ayokunleen_ZA
dc.date.accessioned2015-01-01T12:42:15Z
dc.date.available2015-01-01T12:42:15Z
dc.date.issued2008en_ZA
dc.descriptionIncludes abstract.en_ZA
dc.descriptionIncludes bibliographical references (p. 127-138).en_ZA
dc.description.abstractThis thesis presents the investigation of the production of a novel thermostable amidase (EC 3.5.1.4) from Geobacillus pallidus RAPc8 using recombinant E.coli BL21 (DE3). The choice of growth medium and induction strategy were optimised under bioreactor conditions to enhance amidase productivity. Further, expanded bed adsorption (EBA) was assessed as a tool for minimising the unit operations in the amidase purification train. The EBA process can integrate up to four steps by merging processes involved in centrifugation, microfiltration and initial adsorption into one unit operation.en_ZA
dc.identifier.apacitationOlaofe, O. A. (2008). <i>Process integration in the optimisation of amidase production from recombinant Escherichia coli</i>. (Thesis). University of Cape Town ,Faculty of Engineering & the Built Environment ,Department of Mechanical Engineering. Retrieved from http://hdl.handle.net/11427/10857en_ZA
dc.identifier.chicagocitationOlaofe, Oluwafemi Ayokunle. <i>"Process integration in the optimisation of amidase production from recombinant Escherichia coli."</i> Thesis., University of Cape Town ,Faculty of Engineering & the Built Environment ,Department of Mechanical Engineering, 2008. http://hdl.handle.net/11427/10857en_ZA
dc.identifier.citationOlaofe, O. 2008. Process integration in the optimisation of amidase production from recombinant Escherichia coli. University of Cape Town.en_ZA
dc.identifier.risTY - Thesis / Dissertation AU - Olaofe, Oluwafemi Ayokunle AB - This thesis presents the investigation of the production of a novel thermostable amidase (EC 3.5.1.4) from Geobacillus pallidus RAPc8 using recombinant E.coli BL21 (DE3). The choice of growth medium and induction strategy were optimised under bioreactor conditions to enhance amidase productivity. Further, expanded bed adsorption (EBA) was assessed as a tool for minimising the unit operations in the amidase purification train. The EBA process can integrate up to four steps by merging processes involved in centrifugation, microfiltration and initial adsorption into one unit operation. DA - 2008 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2008 T1 - Process integration in the optimisation of amidase production from recombinant Escherichia coli TI - Process integration in the optimisation of amidase production from recombinant Escherichia coli UR - http://hdl.handle.net/11427/10857 ER -en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/10857
dc.identifier.vancouvercitationOlaofe OA. Process integration in the optimisation of amidase production from recombinant Escherichia coli. [Thesis]. University of Cape Town ,Faculty of Engineering & the Built Environment ,Department of Mechanical Engineering, 2008 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/10857en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentCentre for Bioprocess Engineering Researchen_ZA
dc.publisher.facultyFaculty of Engineering and the Built Environment
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherBioprocess Engineeringen_ZA
dc.titleProcess integration in the optimisation of amidase production from recombinant Escherichia colien_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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