An AraC/XylS family transcriptional regulator homologue from Bacteroides fragilis is associated with cell survival following DNA damage

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dc.contributor.authorCasanueva, Ana I
dc.contributor.authorPaul, Lynthia
dc.contributor.authorPatrick, Sheila
dc.contributor.authorAbratt, Valerie R
dc.date.accessioned2016-07-26T08:37:05Z
dc.date.available2016-07-26T08:37:05Z
dc.date.issued2008
dc.date.updated2016-07-13T11:28:31Z
dc.description.abstractA putative transcriptional regulator of the AraC/XylS family was identified in a genomic genebank of Bacteroides fragilis Bf-1, which partially relieved the sensitivity of Escherichia coli DNA repair mutants to the DNA-damaging agents, metronidazole and mitomycin C. A homologue of this gene with the same phenotype was identified as BF638R3281 in B. fragilis 638R. Transcription of BF638R3281 was constitutive with respect to exposure to sublethal doses of metronidazole. BF638R3281 was interrupted by single cross-over gene-specific insertion mutation, and the gene disruption was confirmed by PCR and DNAsequencing analysis. The mutant grew more slowly than the wild type, and the mutation rendered B. fragilis more sensitive to metronidazole and mitomycin C. This indicates that the BF638R3281 gene product plays a role in the survival of B. fragilis following DNA damage by these agents.en_ZA
dc.identifierhttp://dx.doi.org/10.1111/j.1574-6968.2007.01004.x
dc.identifier.apacitationCasanueva, A. I., Paul, L., Patrick, S., & Abratt, V. R. (2008). An AraC/XylS family transcriptional regulator homologue from Bacteroides fragilis is associated with cell survival following DNA damage. <i>FEMS Microbiology Letters</i>, http://hdl.handle.net/11427/20740en_ZA
dc.identifier.chicagocitationCasanueva, Ana I, Lynthia Paul, Sheila Patrick, and Valerie R Abratt "An AraC/XylS family transcriptional regulator homologue from Bacteroides fragilis is associated with cell survival following DNA damage." <i>FEMS Microbiology Letters</i> (2008) http://hdl.handle.net/11427/20740en_ZA
dc.identifier.citationCasanueva, A. I., Paul, L., Patrick, S., & Abratt, V. R. (2008). An AraC/XylS family transcriptional regulator homologue from Bacteroides fragilis is associated with cell survival following DNA damage. FEMS microbiology letters, 278(2), 249-256.en_ZA
dc.identifier.issn0378-1097en_ZA
dc.identifier.ris TY - Journal Article AU - Casanueva, Ana I AU - Paul, Lynthia AU - Patrick, Sheila AU - Abratt, Valerie R AB - A putative transcriptional regulator of the AraC/XylS family was identified in a genomic genebank of Bacteroides fragilis Bf-1, which partially relieved the sensitivity of Escherichia coli DNA repair mutants to the DNA-damaging agents, metronidazole and mitomycin C. A homologue of this gene with the same phenotype was identified as BF638R3281 in B. fragilis 638R. Transcription of BF638R3281 was constitutive with respect to exposure to sublethal doses of metronidazole. BF638R3281 was interrupted by single cross-over gene-specific insertion mutation, and the gene disruption was confirmed by PCR and DNAsequencing analysis. The mutant grew more slowly than the wild type, and the mutation rendered B. fragilis more sensitive to metronidazole and mitomycin C. This indicates that the BF638R3281 gene product plays a role in the survival of B. fragilis following DNA damage by these agents. DA - 2008 DB - OpenUCT DP - University of Cape Town J1 - FEMS Microbiology Letters LK - https://open.uct.ac.za PB - University of Cape Town PY - 2008 SM - 0378-1097 T1 - An AraC/XylS family transcriptional regulator homologue from Bacteroides fragilis is associated with cell survival following DNA damage TI - An AraC/XylS family transcriptional regulator homologue from Bacteroides fragilis is associated with cell survival following DNA damage UR - http://hdl.handle.net/11427/20740 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/20740
dc.identifier.vancouvercitationCasanueva AI, Paul L, Patrick S, Abratt VR. An AraC/XylS family transcriptional regulator homologue from Bacteroides fragilis is associated with cell survival following DNA damage. FEMS Microbiology Letters. 2008; http://hdl.handle.net/11427/20740.en_ZA
dc.languageengen_ZA
dc.publisherOxford University Pressen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.sourceFEMS Microbiology Lettersen_ZA
dc.source.urihttp://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1574-6968
dc.subject.otherBacteroides fragilis
dc.subject.otherDNA damage
dc.subject.otherTranscriptional regulator
dc.subject.otherMetronidazole
dc.subject.otherMitomycin C
dc.titleAn AraC/XylS family transcriptional regulator homologue from Bacteroides fragilis is associated with cell survival following DNA damageen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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