Characterization of CD8 T cell responses in Mycobacterium Tuberculosis infection

Simple item page
dc.contributor.advisorDay, Cherylen_ZA
dc.contributor.advisorHanekom, Willem Aen_ZA
dc.contributor.authorMoshi, Noell Dominikaen_ZA
dc.date.accessioned2015-01-08T19:59:34Z
dc.date.available2015-01-08T19:59:34Z
dc.date.issued2011en_ZA
dc.description.abstractThe aim of this project was to compare the breadth and magnitude of CFP10 and ESAT6-specific CD8 T cell responses in individuals with latent Mycobacterium tuberculosis (MTB) infection (LTBI) and active TB disease, and further define MTB-specific CD8 T cell phenotypes associated with latent infection and active disease. Ex vivo IFN? Elispots and proliferation assays were used to identify immunodominant ESAT6 and CFP10 15mer peptides targeted by CD8 T cells in LTBI and TB donors. A multiparameter flow cytometry panel was designed and optimized to assess turnover, susceptibility to apoptosis and terminal differentiation/senescence in CD8 T cells from TB and LTBI donors. Bcl-2, Ki67,CD95, CD57, CD127 and IFNγ were thus measured in each group.en_ZA
dc.identifier.apacitationMoshi, N. D. (2011). <i>Characterization of CD8 T cell responses in Mycobacterium Tuberculosis infection</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Immunology. Retrieved from http://hdl.handle.net/11427/11785en_ZA
dc.identifier.chicagocitationMoshi, Noell Dominika. <i>"Characterization of CD8 T cell responses in Mycobacterium Tuberculosis infection."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Immunology, 2011. http://hdl.handle.net/11427/11785en_ZA
dc.identifier.citationMoshi, N. 2011. Characterization of CD8 T cell responses in Mycobacterium Tuberculosis infection. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Moshi, Noell Dominika AB - The aim of this project was to compare the breadth and magnitude of CFP10 and ESAT6-specific CD8 T cell responses in individuals with latent Mycobacterium tuberculosis (MTB) infection (LTBI) and active TB disease, and further define MTB-specific CD8 T cell phenotypes associated with latent infection and active disease. Ex vivo IFN? Elispots and proliferation assays were used to identify immunodominant ESAT6 and CFP10 15mer peptides targeted by CD8 T cells in LTBI and TB donors. A multiparameter flow cytometry panel was designed and optimized to assess turnover, susceptibility to apoptosis and terminal differentiation/senescence in CD8 T cells from TB and LTBI donors. Bcl-2, Ki67,CD95, CD57, CD127 and IFN&#947; were thus measured in each group. DA - 2011 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2011 T1 - Characterization of CD8 T cell responses in Mycobacterium Tuberculosis infection TI - Characterization of CD8 T cell responses in Mycobacterium Tuberculosis infection UR - http://hdl.handle.net/11427/11785 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/11785
dc.identifier.vancouvercitationMoshi ND. Characterization of CD8 T cell responses in Mycobacterium Tuberculosis infection. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Immunology, 2011 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/11785en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Immunologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherClinical Science and Immunologyen_ZA
dc.titleCharacterization of CD8 T cell responses in Mycobacterium Tuberculosis infectionen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
thesis_hsf_2011_moshi_n.pdf
Size:
5.88 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Masters