Phenotypic analysis of peripheral B cell populations during Mycobacterium tuberculosis infection and disease

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dc.contributor.authordu Plessis, Willem J
dc.contributor.authorKeyser, Alana
dc.contributor.authorWalzl, Gerhard
dc.contributor.authorLoxton, André G
dc.date.accessioned2021-10-08T07:08:14Z
dc.date.available2021-10-08T07:08:14Z
dc.date.issued2016
dc.description.abstractAbstract Background Mycobacterium tuberculosis (Mtb) remains an unresolved threat resulting in great annual loss of life. The role of B cells during the protective immunity to Mtb is still unclear. B cells have been described as effector cells in addition to their role as antibody producing cells during disease. Here we aim to identify and characterize the frequency of peripheral B-cell subpopulations during active Tuberculosis and over treatment response. Analysis were done for both class switched (CS) and non-class switched (NCS) phenotypes. Methods We recruited participants with active untreated pulmonary Tuberculosis, other lung diseases and healthy community controls. All groups were followed up for one week from recruitment and the TB cases till the end of treatment (month 6). Results Peripheral blood samples were collected, stained with monoclonal antibodies to CD19 + cells, Immunoglobulin (Ig) M, plasma cells (CD 138 + ), marker of memory (CD27 + ), immune activation (CD23 + ) and acquired on a flow cytometer. Circulating Marginal zone B cells (CD19 + IgM + CD23 − CD27 + ) and memory phenotypes are able to distinguish between TB diagnosis and end of treatment. The frequency of mature B cells from TB cases are lower than that of other-lung diseases at diagnosis. A subpopulation of activated memory B cells (CD19 + IgM + CD23 + CD27 + ) cells are present at the end of TB treatment. Conclusions This study identified distinctive B cell subpopulations present during active TB disease and other lung disease conditions. These cell populations warrants further examination in larger studies as it may be informative as cell markers or as effectors/regulators in TB disease or TB treatment response.
dc.identifier.apacitationdu Plessis, W. J., Keyser, A., Walzl, G., & Loxton, A. G. (2016). Phenotypic analysis of peripheral B cell populations during Mycobacterium tuberculosis infection and disease. <i>Journal of Inflammation (London)</i>, 13(1), 174 - 177. http://hdl.handle.net/11427/34531en_ZA
dc.identifier.chicagocitationdu Plessis, Willem J, Alana Keyser, Gerhard Walzl, and André G Loxton "Phenotypic analysis of peripheral B cell populations during Mycobacterium tuberculosis infection and disease." <i>Journal of Inflammation (London)</i> 13, 1. (2016): 174 - 177. http://hdl.handle.net/11427/34531en_ZA
dc.identifier.citationdu Plessis, W.J., Keyser, A., Walzl, G. & Loxton, A.G. 2016. Phenotypic analysis of peripheral B cell populations during Mycobacterium tuberculosis infection and disease. <i>Journal of Inflammation (London).</i> 13(1):174 - 177. http://hdl.handle.net/11427/34531en_ZA
dc.identifier.issn1476-9255
dc.identifier.ris TY - Journal Article AU - du Plessis, Willem J AU - Keyser, Alana AU - Walzl, Gerhard AU - Loxton, André G AB - Abstract Background Mycobacterium tuberculosis (Mtb) remains an unresolved threat resulting in great annual loss of life. The role of B cells during the protective immunity to Mtb is still unclear. B cells have been described as effector cells in addition to their role as antibody producing cells during disease. Here we aim to identify and characterize the frequency of peripheral B-cell subpopulations during active Tuberculosis and over treatment response. Analysis were done for both class switched (CS) and non-class switched (NCS) phenotypes. Methods We recruited participants with active untreated pulmonary Tuberculosis, other lung diseases and healthy community controls. All groups were followed up for one week from recruitment and the TB cases till the end of treatment (month 6). Results Peripheral blood samples were collected, stained with monoclonal antibodies to CD19 + cells, Immunoglobulin (Ig) M, plasma cells (CD 138 + ), marker of memory (CD27 + ), immune activation (CD23 + ) and acquired on a flow cytometer. Circulating Marginal zone B cells (CD19 + IgM + CD23 − CD27 + ) and memory phenotypes are able to distinguish between TB diagnosis and end of treatment. The frequency of mature B cells from TB cases are lower than that of other-lung diseases at diagnosis. A subpopulation of activated memory B cells (CD19 + IgM + CD23 + CD27 + ) cells are present at the end of TB treatment. Conclusions This study identified distinctive B cell subpopulations present during active TB disease and other lung disease conditions. These cell populations warrants further examination in larger studies as it may be informative as cell markers or as effectors/regulators in TB disease or TB treatment response. DA - 2016 DB - OpenUCT DP - University of Cape Town IS - 1 J1 - Journal of Inflammation (London) LK - https://open.uct.ac.za PY - 2016 SM - 1476-9255 T1 - Phenotypic analysis of peripheral B cell populations during Mycobacterium tuberculosis infection and disease TI - Phenotypic analysis of peripheral B cell populations during Mycobacterium tuberculosis infection and disease UR - http://hdl.handle.net/11427/34531 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/34531
dc.identifier.vancouvercitationdu Plessis WJ, Keyser A, Walzl G, Loxton AG. Phenotypic analysis of peripheral B cell populations during Mycobacterium tuberculosis infection and disease. Journal of Inflammation (London). 2016;13(1):174 - 177. http://hdl.handle.net/11427/34531.en_ZA
dc.language.isoeng
dc.publisher.departmentDivision of Human Genetics
dc.publisher.facultyFaculty of Health Sciences
dc.sourceJournal of Inflammation (London)
dc.source.journalissue1
dc.source.journalvolume13
dc.source.pagination174 - 177
dc.source.urihttps://dx.doi.org/10.1186/s12950-016-0133-4
dc.subject.otherImmunology
dc.subject.otherAllergology
dc.subject.otherCytokines and Growth Factors
dc.subject.otherRheumatology
dc.subject.otherPharmacology/Toxicology
dc.subject.otherGastroenterology
dc.titlePhenotypic analysis of peripheral B cell populations during Mycobacterium tuberculosis infection and disease
dc.typeJournal Article
uct.type.publicationResearch
uct.type.resourceJournal Article
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