An investigation of the molecular biology and genetics of Witches' Broom disease of Protea cynaroides

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dc.contributor.advisorWoods, David Ren_ZA
dc.contributor.authorDorrington, Rosemary Annen_ZA
dc.date.accessioned2014-09-22T07:53:48Z
dc.date.available2014-09-22T07:53:48Z
dc.date.issued1987en_ZA
dc.descriptionBibliography: leaves 102-117en_ZA
dc.description.abstractAn investigation was undertaken into the biology and genetics of Witches' Broom disease on Protea cynaroides. The investigation was approached in two ways: firstly, from a physiological and pathological angle and secondly at the genetic level. As very little is known about the causes of Witches' Broom disease on P. cynaroides, an attempt was made to identify a pathogen which could be held responsible for the disease. A number of plants were studied in the field and from these samples were taken and cultured on culture medium. Healthy P. cynaroides tissue was not established in tissue culture, while more success was obtained with teratoma tissue. Attempts were made to transmit the disease but these were unsuccessful. Four strains of Agrobacterium tumefaciens were unable to induce tumours on P. cynaroides seedlings. Sections of vascular tissue from teratoma and healthy tissue were viewed under the electron microscope but revealed no pathogen.en_ZA
dc.identifier.apacitationDorrington, R. A. (1987). <i>An investigation of the molecular biology and genetics of Witches' Broom disease of Protea cynaroides</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/7597en_ZA
dc.identifier.chicagocitationDorrington, Rosemary Ann. <i>"An investigation of the molecular biology and genetics of Witches' Broom disease of Protea cynaroides."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1987. http://hdl.handle.net/11427/7597en_ZA
dc.identifier.citationDorrington, R. 1987. An investigation of the molecular biology and genetics of Witches' Broom disease of Protea cynaroides. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Dorrington, Rosemary Ann AB - An investigation was undertaken into the biology and genetics of Witches' Broom disease on Protea cynaroides. The investigation was approached in two ways: firstly, from a physiological and pathological angle and secondly at the genetic level. As very little is known about the causes of Witches' Broom disease on P. cynaroides, an attempt was made to identify a pathogen which could be held responsible for the disease. A number of plants were studied in the field and from these samples were taken and cultured on culture medium. Healthy P. cynaroides tissue was not established in tissue culture, while more success was obtained with teratoma tissue. Attempts were made to transmit the disease but these were unsuccessful. Four strains of Agrobacterium tumefaciens were unable to induce tumours on P. cynaroides seedlings. Sections of vascular tissue from teratoma and healthy tissue were viewed under the electron microscope but revealed no pathogen. DA - 1987 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1987 T1 - An investigation of the molecular biology and genetics of Witches' Broom disease of Protea cynaroides TI - An investigation of the molecular biology and genetics of Witches' Broom disease of Protea cynaroides UR - http://hdl.handle.net/11427/7597 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/7597
dc.identifier.vancouvercitationDorrington RA. An investigation of the molecular biology and genetics of Witches' Broom disease of Protea cynaroides. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1987 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/7597en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherScienceen_ZA
dc.titleAn investigation of the molecular biology and genetics of Witches' Broom disease of Protea cynaroidesen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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