Genetic Manipulation of Non-tuberculosis Mycobacteria

Simple item page
dc.contributor.authorChimukuche, Nyaradzai
dc.contributor.authorWilliams, Monique Joy
dc.date.accessioned2021-05-19T12:11:09Z
dc.date.available2021-05-19T12:11:09Z
dc.date.issued2021-02-17
dc.description.abstractNon-tuberculosis mycobacteria (NTMs) comprise a large group of organisms that are phenotypically diverse. Analysis of the growing number of completed NTM genomes has revealed both significant intra-genus genetic diversity, and a high percentage of predicted genes that appear to be unique to this group. Most NTMs have not been studied, however, the rise in NTM infections in several countries has prompted increasing interest in these organisms. Mycobacterial research has recently benefitted from the development of new genetic tools and a growing number of studies describing the genetic manipulation of NTMs have now been reported. In this review, we discuss the use of both site-specific and random mutagenesis tools in NTMs, highlighting the challenges that exist in applying these techniques to this diverse group of organisms.en_US
dc.identifier.apacitationChimukuche, N., & Williams, M. J. (2021). Genetic Manipulation of Non-tuberculosis Mycobacteria. <i>Frontiers in Microbiology</i>, 12 http://hdl.handle.net/11427/33237en_ZA
dc.identifier.chicagocitationChimukuche, Nyaradzai, and Monique Joy Williams "Genetic Manipulation of Non-tuberculosis Mycobacteria." <i>Frontiers in Microbiology</i> 12, (2021) http://hdl.handle.net/11427/33237en_ZA
dc.identifier.citationChimukuche, N. & Williams, M.J. 2021. Genetic Manipulation of Non-tuberculosis Mycobacteria. <i>Frontiers in Microbiology.</i> 12 http://hdl.handle.net/11427/33237en_ZA
dc.identifier.risTY - Journal Article AU - Chimukuche, Nyaradzai AU - Williams, Monique Joy AB - Non-tuberculosis mycobacteria (NTMs) comprise a large group of organisms that are phenotypically diverse. Analysis of the growing number of completed NTM genomes has revealed both significant intra-genus genetic diversity, and a high percentage of predicted genes that appear to be unique to this group. Most NTMs have not been studied, however, the rise in NTM infections in several countries has prompted increasing interest in these organisms. Mycobacterial research has recently benefitted from the development of new genetic tools and a growing number of studies describing the genetic manipulation of NTMs have now been reported. In this review, we discuss the use of both site-specific and random mutagenesis tools in NTMs, highlighting the challenges that exist in applying these techniques to this diverse group of organisms. DA - 2021-02-17 DB - OpenUCT DP - University of Cape Town J1 - Frontiers in Microbiology KW - non-tuberculosis mycobacteria (NTMs), mutagenesis, homologous recombination, recombineering, transposon mutagenesis, CRISPR/Cas LK - https://open.uct.ac.za PY - 2021 T1 - Genetic Manipulation of Non-tuberculosis Mycobacteria TI - Genetic Manipulation of Non-tuberculosis Mycobacteria UR - http://hdl.handle.net/11427/33237 ER -en_ZA
dc.identifier.uri10.3389/fmicb.2021.633510
dc.identifier.urihttp://hdl.handle.net/11427/33237
dc.identifier.vancouvercitationChimukuche N, Williams MJ. Genetic Manipulation of Non-tuberculosis Mycobacteria. Frontiers in Microbiology. 2021;12 http://hdl.handle.net/11427/33237.en_ZA
dc.language.isoenen_US
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_US
dc.publisher.facultyFaculty of Scienceen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.sourceFrontiers in Microbiologyen_US
dc.source.journalvolume12
dc.source.urihttps://www.frontiersin.org/journals/microbiology
dc.subjectnon-tuberculosis mycobacteria (NTMs)
dc.subjectmutagenesis
dc.subjecthomologous recombination
dc.subjectrecombineering
dc.subjecttransposon mutagenesis
dc.subjectCRISPR/Cas
dc.titleGenetic Manipulation of Non-tuberculosis Mycobacteriaen_US
dc.typeJournal Articleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
fmicb-12-633510.pdf
Size:
888.02 KB
Format:
Adobe Portable Document Format
Description:
Review article
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.72 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Journal Articles