The reaction between ferrocytochrome c and hydrogen peroxide

Simple item page
dc.contributor.advisorAhrens, L Hen_ZA
dc.contributor.advisorErlank, A Jen_ZA
dc.contributor.authorHulett, Leslie Grahamen_ZA
dc.date.accessioned2016-10-25T13:36:32Z
dc.date.available2016-10-25T13:36:32Z
dc.date.issued1968en_ZA
dc.description.abstractThe reaction between ferrocytochrome c and hydrogen peroxide has been studied to determine possible reaction mechanisms. This was done by investigating the effect of various physical factors, such as hydrogen ion concentration, ionic strength and temperature, on the reaction and by studying the kinetics of the reaction. The study of pH and ionic strength showed that an ionization occurred at pH 7.8 which influenced the reactivity of the cytochrome c. The reaction was studied kinetically at pH 8.1 and pH 6.6. At pH 8.1 simple kinetics showed an uncomplicated reaction between ferrocytochrome c and hydrogen peroxide. The kinetic studies at pH 6.6 showed that ferrocytochrome c, hydrogen peroxide and ferricytochrome c (acting as a product catalyst) were involved in the reaction. The product catalysis complicated the kinetics and difficulty was experienced in fitting a suitable reaction mechanism. Copper ions have been found to catalyse reactions similar to that studied here. The effect of a copper histidine complex was therefore investigated on the reaction at pH 8.7. The copper complex was found to catalyse the oxidation of ferrocytochrome c by hydrogen peroxide and the kinetic studies of this oxidation showed that the reaction could be fitted to a simple mechanism which did not involve the formation of intermediate complexes. An unusual feature of the reaction was the ability of the cytochrome c, after complete oxidation, to undergo reduction, which is dependent on the presence of both hydrogen peroxide and copper ions.en_ZA
dc.identifier.apacitationHulett, L. G. (1968). <i>The reaction between ferrocytochrome c and hydrogen peroxide</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Geological Sciences. Retrieved from http://hdl.handle.net/11427/22300en_ZA
dc.identifier.chicagocitationHulett, Leslie Graham. <i>"The reaction between ferrocytochrome c and hydrogen peroxide."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Geological Sciences, 1968. http://hdl.handle.net/11427/22300en_ZA
dc.identifier.citationHulett, L. 1968. The reaction between ferrocytochrome c and hydrogen peroxide. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Hulett, Leslie Graham AB - The reaction between ferrocytochrome c and hydrogen peroxide has been studied to determine possible reaction mechanisms. This was done by investigating the effect of various physical factors, such as hydrogen ion concentration, ionic strength and temperature, on the reaction and by studying the kinetics of the reaction. The study of pH and ionic strength showed that an ionization occurred at pH 7.8 which influenced the reactivity of the cytochrome c. The reaction was studied kinetically at pH 8.1 and pH 6.6. At pH 8.1 simple kinetics showed an uncomplicated reaction between ferrocytochrome c and hydrogen peroxide. The kinetic studies at pH 6.6 showed that ferrocytochrome c, hydrogen peroxide and ferricytochrome c (acting as a product catalyst) were involved in the reaction. The product catalysis complicated the kinetics and difficulty was experienced in fitting a suitable reaction mechanism. Copper ions have been found to catalyse reactions similar to that studied here. The effect of a copper histidine complex was therefore investigated on the reaction at pH 8.7. The copper complex was found to catalyse the oxidation of ferrocytochrome c by hydrogen peroxide and the kinetic studies of this oxidation showed that the reaction could be fitted to a simple mechanism which did not involve the formation of intermediate complexes. An unusual feature of the reaction was the ability of the cytochrome c, after complete oxidation, to undergo reduction, which is dependent on the presence of both hydrogen peroxide and copper ions. DA - 1968 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1968 T1 - The reaction between ferrocytochrome c and hydrogen peroxide TI - The reaction between ferrocytochrome c and hydrogen peroxide UR - http://hdl.handle.net/11427/22300 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/22300
dc.identifier.vancouvercitationHulett LG. The reaction between ferrocytochrome c and hydrogen peroxide. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Geological Sciences, 1968 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/22300en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Geological Sciencesen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherGeochemistryen_ZA
dc.titleThe reaction between ferrocytochrome c and hydrogen peroxideen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
thesis_sci_1968_hulett_leslie_graham.pdf
Size:
4.58 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Masters