Effect of female genital schistosomiasis and anti-schistosomal treatment on monocytes, CD4+ T-cells and CCR5 expression in the female genital tract

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dc.contributor.authorKleppa, Elisabethen_ZA
dc.contributor.authorRamsuran, Veronen_ZA
dc.contributor.authorZulu, Siphosenkosien_ZA
dc.contributor.authorKarlsen, Gunn Hegeen_ZA
dc.contributor.authorBere, Alfreden_ZA
dc.contributor.authorPassmore, Jo-Ann Sen_ZA
dc.contributor.authorNdhlovu, Patriciaen_ZA
dc.contributor.authorLillebø, Kristineen_ZA
dc.contributor.authorHolmen, Sigve Den_ZA
dc.contributor.authorOnsrud, Mathiasen_ZA
dc.date.accessioned2015-11-11T14:26:28Z
dc.date.available2015-11-11T14:26:28Z
dc.date.issued2014en_ZA
dc.description.abstractBACKGROUND: Schistosoma haematobium is a waterborne parasite that may cause female genital schistosomiasis (FGS), characterized by genital mucosal lesions. There is clinical and epidemiological evidence for a relationship between FGS and HIV. We investigated the impact of FGS on HIV target cell density and expression of the HIV co-receptor CCR5 in blood and cervical cytobrush samples. Furthermore we evaluated the effect of anti-schistosomal treatment on these cell populations. Design The study followed a case-control design with post treatment follow-up, nested in an on-going field study on FGS. METHODS: Blood and cervical cytobrush samples were collected from FGS negative and positive women for flow cytometry analyses. Urine samples were investigated for schistosome ova by microscopy and polymerase chain reaction (PCR). RESULTS: FGS was associated with a higher frequency of CD14 + cells (monocytes) in blood (11.5% in FGS+ vs. 2.2% in FGS-, p = 0.042). Frequencies of CD4 + cells expressing CCR5 were higher in blood samples from FGS+ than from FGS- women (4.7% vs. 1.5%, p = 0.018). The CD14 + cell population decreased significantly in both compartments after anti-schistosomal treatment (p = 0.043). Although the frequency of CD4+ cells did not change after treatment, frequencies of CCR5 expression by CD4+ cells decreased significantly in both compartments (from 3.4% to 0.5% in blood, p = 0.036; and from 42.4% to 5.6% in genital samples, p = 0.025). CONCLUSIONS: The results support the hypothesis that FGS may increase the risk of HIV acquisition, not only through damage of the mucosal epithelial barrier, but also by affecting HIV target cell populations, and that anti-schistosomal treatment can modify this.en_ZA
dc.identifier.apacitationKleppa, E., Ramsuran, V., Zulu, S., Karlsen, G. H., Bere, A., Passmore, J. S., ... Onsrud, M. (2014). Effect of female genital schistosomiasis and anti-schistosomal treatment on monocytes, CD4+ T-cells and CCR5 expression in the female genital tract. <i>PLoS One</i>, http://hdl.handle.net/11427/14917en_ZA
dc.identifier.chicagocitationKleppa, Elisabeth, Veron Ramsuran, Siphosenkosi Zulu, Gunn Hege Karlsen, Alfred Bere, Jo-Ann S Passmore, Patricia Ndhlovu, Kristine Lillebø, Sigve D Holmen, and Mathias Onsrud "Effect of female genital schistosomiasis and anti-schistosomal treatment on monocytes, CD4+ T-cells and CCR5 expression in the female genital tract." <i>PLoS One</i> (2014) http://hdl.handle.net/11427/14917en_ZA
dc.identifier.citationKleppa, E., Ramsuran, V., Zulu, S., Karlsen, G. H., Bere, A., Passmore, J. A., ... & Ndung'u, T. (2013). Effect of female genital schistosomiasis and anti-schistosomal treatment on monocytes, CD4+ T-cells and CCR5 expression in the female genital tract. PloS one, 9(6), e98593. doi:10.1371/journal.pone.0098593en_ZA
dc.identifier.ris TY - Journal Article AU - Kleppa, Elisabeth AU - Ramsuran, Veron AU - Zulu, Siphosenkosi AU - Karlsen, Gunn Hege AU - Bere, Alfred AU - Passmore, Jo-Ann S AU - Ndhlovu, Patricia AU - Lillebø, Kristine AU - Holmen, Sigve D AU - Onsrud, Mathias AB - BACKGROUND: Schistosoma haematobium is a waterborne parasite that may cause female genital schistosomiasis (FGS), characterized by genital mucosal lesions. There is clinical and epidemiological evidence for a relationship between FGS and HIV. We investigated the impact of FGS on HIV target cell density and expression of the HIV co-receptor CCR5 in blood and cervical cytobrush samples. Furthermore we evaluated the effect of anti-schistosomal treatment on these cell populations. Design The study followed a case-control design with post treatment follow-up, nested in an on-going field study on FGS. METHODS: Blood and cervical cytobrush samples were collected from FGS negative and positive women for flow cytometry analyses. Urine samples were investigated for schistosome ova by microscopy and polymerase chain reaction (PCR). RESULTS: FGS was associated with a higher frequency of CD14 + cells (monocytes) in blood (11.5% in FGS+ vs. 2.2% in FGS-, p = 0.042). Frequencies of CD4 + cells expressing CCR5 were higher in blood samples from FGS+ than from FGS- women (4.7% vs. 1.5%, p = 0.018). The CD14 + cell population decreased significantly in both compartments after anti-schistosomal treatment (p = 0.043). Although the frequency of CD4+ cells did not change after treatment, frequencies of CCR5 expression by CD4+ cells decreased significantly in both compartments (from 3.4% to 0.5% in blood, p = 0.036; and from 42.4% to 5.6% in genital samples, p = 0.025). CONCLUSIONS: The results support the hypothesis that FGS may increase the risk of HIV acquisition, not only through damage of the mucosal epithelial barrier, but also by affecting HIV target cell populations, and that anti-schistosomal treatment can modify this. DA - 2014 DB - OpenUCT DO - 10.1371/journal.pone.0098593 DP - University of Cape Town J1 - PLoS One LK - https://open.uct.ac.za PB - University of Cape Town PY - 2014 T1 - Effect of female genital schistosomiasis and anti-schistosomal treatment on monocytes, CD4+ T-cells and CCR5 expression in the female genital tract TI - Effect of female genital schistosomiasis and anti-schistosomal treatment on monocytes, CD4+ T-cells and CCR5 expression in the female genital tract UR - http://hdl.handle.net/11427/14917 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/14917
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pone.0098593
dc.identifier.vancouvercitationKleppa E, Ramsuran V, Zulu S, Karlsen GH, Bere A, Passmore JS, et al. Effect of female genital schistosomiasis and anti-schistosomal treatment on monocytes, CD4+ T-cells and CCR5 expression in the female genital tract. PLoS One. 2014; http://hdl.handle.net/11427/14917.en_ZA
dc.language.isoengen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.publisher.departmentDivision of Virologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_ZA
dc.rights.holder© 2014 Kleppa et alen_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/4.0en_ZA
dc.sourcePLoS Oneen_ZA
dc.source.urihttp://journals.plos.org/plosoneen_ZA
dc.subject.otherHIVen_ZA
dc.subject.otherSchistosomiasisen_ZA
dc.subject.otherT cellsen_ZA
dc.subject.otherMonocytesen_ZA
dc.subject.otherSchistosomaen_ZA
dc.subject.otherHIV infectionsen_ZA
dc.titleEffect of female genital schistosomiasis and anti-schistosomal treatment on monocytes, CD4+ T-cells and CCR5 expression in the female genital tracten_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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