Production and characterization of alkaliphilic amylases from Bacillus halodurans Alk36

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dc.contributor.advisorHarrison, STLen_ZA
dc.contributor.advisorFenner, Carynen_ZA
dc.contributor.authorMrisho, Latifa Mbwanaen_ZA
dc.date.accessioned2016-06-22T08:57:55Z
dc.date.available2016-06-22T08:57:55Z
dc.date.issued2015en_ZA
dc.description.abstractAmylases are hydrolytic enzymes that cause the breakdown of starch and related polysaccharides to simple sugars. Amylases are applied in brewing, food, detergent and textile industries. Most commercial amylases are derived from fungi or bacteria. Bacterial amylases are desired for commercial use, due to their thermo-stability and faster production rates. Bacteria of the genus, Bacillus, are considered to be a good source of extracellular proteins because they have high growth rates and have a naturally high capacity for secretion of extracellular proteins. Bacillus halodurans Alk36 is an alkaliphilic, thermotolerant isolate that can grow over a wide pH and temperature range. Preliminary studies have shown that B. halodurans Alk36 can grown in EnBase® medium (at pH 8.5) containing starch as the carbon source, without the addition of a commercial amylase. The ability to grow on starch, in the absence of an external amylase, indicated that this strain produces endogenous alkaliphilic amylases, which may be exploited for a number of industrial applications. In the present study, the physiological and biochemical characterisation of B. halodurans Alk36 and its endogenous amylases were investigated.en_ZA
dc.identifier.apacitationMrisho, L. M. (2015). <i>Production and characterization of alkaliphilic amylases from Bacillus halodurans Alk36</i>. (Thesis). University of Cape Town ,Faculty of Engineering and the Built Environment ,Centre for Bioprocess Engineering Research. Retrieved from http://hdl.handle.net/11427/20089en_ZA
dc.identifier.chicagocitationMrisho, Latifa Mbwana. <i>"Production and characterization of alkaliphilic amylases from Bacillus halodurans Alk36."</i> Thesis., University of Cape Town ,Faculty of Engineering and the Built Environment ,Centre for Bioprocess Engineering Research, 2015. http://hdl.handle.net/11427/20089en_ZA
dc.identifier.citationMrisho, L.M. 2015. Production and characterization of alkaliphilic amylases from Bacillus halodurans Alk36. Thesis. University of Cape Town ,Faculty of Engineering and the Built Environment ,Centre for Bioprocess Engineering Research. http://hdl.handle.net/11427/20089en_ZA
dc.identifier.risTY - Thesis / Dissertation AU - Mrisho, Latifa Mbwana AB - Amylases are hydrolytic enzymes that cause the breakdown of starch and related polysaccharides to simple sugars. Amylases are applied in brewing, food, detergent and textile industries. Most commercial amylases are derived from fungi or bacteria. Bacterial amylases are desired for commercial use, due to their thermo-stability and faster production rates. Bacteria of the genus, Bacillus, are considered to be a good source of extracellular proteins because they have high growth rates and have a naturally high capacity for secretion of extracellular proteins. Bacillus halodurans Alk36 is an alkaliphilic, thermotolerant isolate that can grow over a wide pH and temperature range. Preliminary studies have shown that B. halodurans Alk36 can grown in EnBase® medium (at pH 8.5) containing starch as the carbon source, without the addition of a commercial amylase. The ability to grow on starch, in the absence of an external amylase, indicated that this strain produces endogenous alkaliphilic amylases, which may be exploited for a number of industrial applications. In the present study, the physiological and biochemical characterisation of B. halodurans Alk36 and its endogenous amylases were investigated. DA - 2015 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2015 T1 - Production and characterization of alkaliphilic amylases from Bacillus halodurans Alk36 TI - Production and characterization of alkaliphilic amylases from Bacillus halodurans Alk36 UR - http://hdl.handle.net/11427/20089 ER -en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/20089
dc.identifier.vancouvercitationMrisho LM. Production and characterization of alkaliphilic amylases from Bacillus halodurans Alk36. [Thesis]. University of Cape Town ,Faculty of Engineering and the Built Environment ,Centre for Bioprocess Engineering Research, 2015 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/20089en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentCentre for Bioprocess Engineering Researchen_ZA
dc.publisher.facultyFaculty of Engineering and the Built Environment
dc.publisher.institutionUniversity of Cape Town
dc.subjectBioprocess Engineering
dc.titleProduction and characterization of alkaliphilic amylases from Bacillus halodurans Alk36en_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMSc (Eng)en_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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