Beak and feather disease virus candidate vaccine development

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dc.contributor.advisorRybicki, Eden_ZA
dc.contributor.advisorHitzeroth, Ingaen_ZA
dc.contributor.advisorMeyers, Annen_ZA
dc.contributor.authorDuvenage, Lucianen_ZA
dc.date.accessioned2015-05-13T14:15:55Z
dc.date.available2015-05-13T14:15:55Z
dc.date.issued2012en_ZA
dc.descriptionIncludes bibliographical references.en_ZA
dc.description.abstract[Fix supervisors field.] Psittacine beak and feather disease, caused by a circovirus known as beak and feather disease virus (BFDV), is a threat to both wild and captive psittacine species. There is currently no vaccine against BFDV and safe and affordable vaccine candidates are needed to alleviate the disease burden caused by this virus. Production of the BFDV's major antigenic determinant, the capsid protein (CP), in the inexpensive and highly scalable plant expression system, could satisfy these requirements as a potential subunit vaccine. In this work, truncated CP (ÄN40 CP) was first expressed in E. coli to successfully generate anti-CP polyclonal antibodies. ÄN40 CP and full-length CP transient expression in tobacco (Nicotiana benthamiana) was optimised as fusions to elastin-like polypeptide (ELP). Fusion of CP or ÄN40 CP to ELPs of different lengths was shown to increase yield relative to unfused CP/ÄN40 CP. Free ELP and a GFP-ELP fusion could be purified by inverse transition cycling (ITC), using centrifugation and membrane filtration methods. A ÄN40 CP-ELP fusion expressed in plants could be partially purified and represents low-cost vaccine candidate against BFDV. A candidate DNA vaccine expressing ÄN40 CP was also evaluated for expression of the antigen in vitro and may prove useful in a prime-boost regimen together with one of the plant-produced vaccine candidates.en_ZA
dc.identifier.apacitationDuvenage, L. (2012). <i>Beak and feather disease virus candidate vaccine development</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/12785en_ZA
dc.identifier.chicagocitationDuvenage, Lucian. <i>"Beak and feather disease virus candidate vaccine development."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2012. http://hdl.handle.net/11427/12785en_ZA
dc.identifier.citationDuvenage, L. 2012. Beak and feather disease virus candidate vaccine development. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Duvenage, Lucian AB - [Fix supervisors field.] Psittacine beak and feather disease, caused by a circovirus known as beak and feather disease virus (BFDV), is a threat to both wild and captive psittacine species. There is currently no vaccine against BFDV and safe and affordable vaccine candidates are needed to alleviate the disease burden caused by this virus. Production of the BFDV's major antigenic determinant, the capsid protein (CP), in the inexpensive and highly scalable plant expression system, could satisfy these requirements as a potential subunit vaccine. In this work, truncated CP (ÄN40 CP) was first expressed in E. coli to successfully generate anti-CP polyclonal antibodies. ÄN40 CP and full-length CP transient expression in tobacco (Nicotiana benthamiana) was optimised as fusions to elastin-like polypeptide (ELP). Fusion of CP or ÄN40 CP to ELPs of different lengths was shown to increase yield relative to unfused CP/ÄN40 CP. Free ELP and a GFP-ELP fusion could be purified by inverse transition cycling (ITC), using centrifugation and membrane filtration methods. A ÄN40 CP-ELP fusion expressed in plants could be partially purified and represents low-cost vaccine candidate against BFDV. A candidate DNA vaccine expressing ÄN40 CP was also evaluated for expression of the antigen in vitro and may prove useful in a prime-boost regimen together with one of the plant-produced vaccine candidates. DA - 2012 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2012 T1 - Beak and feather disease virus candidate vaccine development TI - Beak and feather disease virus candidate vaccine development UR - http://hdl.handle.net/11427/12785 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/12785
dc.identifier.vancouvercitationDuvenage L. Beak and feather disease virus candidate vaccine development. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2012 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/12785en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMolecular and Cell Biologyen_ZA
dc.titleBeak and feather disease virus candidate vaccine developmenten_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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