Post-translational cleavage of recombinantly expressed nitrilase from Rhodococcus rhodochrous J1 yields a stable, active helical form

Thuku, R Ndoria; Weber, Brandon W; Varsani, Arvind; Sewell, B. Trevor

Post-translational cleavage of recombinantly expressed nitrilase from Rhodococcus rhodochrous J1 yields a stable, active helical form

Journal Article

2007

Journal Title

FEBS Journal

Link to Journal

http://febs.onlinelibrary.wiley.com/hub/journal/10.1111/(ISSN)1742-4658/

Publisher

Wiley

Publisher

University of Cape Town

License

http://creativecommons.org/licenses/by-nc/4.0/

Abstract

Nitrilases convert nitriles to the corresponding carboxylic acids and ammonia. The nitrilase from Rhodococcus rhodochrous J1 is known to be inactive as a dimer but to become active on oligomerization. The recombinant enzyme undergoes post-translational cleavage at approximately residue 327, resulting in the formation of active, helical homo-oligomers. Determining the 3D structure of these helices using electron microscopy, followed by fitting the stain envelope with a model based on homology with other members of the nitrilase superfamily, enables the interacting surfaces to be identified. This also suggests that the reason for formation of the helices is related to the removal of steric hindrance arising from the 39 C-terminal amino acids from the wild-type protein. The helical form can be generated by expressing only residues 1-327.