Genomic distribution of histone H1 in budding yeast (Saccharomyces cerevisiae) : yeast chromosome III

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dc.contributor.advisorPatterton, Hughen_ZA
dc.contributor.authorPriya, Vattem Padmaen_ZA
dc.date.accessioned2014-07-30T17:41:43Z
dc.date.available2014-07-30T17:41:43Z
dc.date.issued2002en_ZA
dc.descriptionIncludes bibliographical references.
dc.description.abstractThe linker histone HI binds to the nucleosome and is essential for the organization of nucleosomes into the 30-nm filament of the chromatin. This compaction of DNA has a well-characterized effect on DNA function. In Saccharomyces cerevisiae, HHO 1 encodes a putative linker histone with very significant homology to histone HI. In vitro chromatin assembly experiments with recombinant Hho 1 p have shown that it is able to complex with the dinucleosomes in a similar manner to histone HI. It has also been reported that disruption of HHOl has little affect on RNA levels. A longstanding issue concerns the location of Hho 1 p in the chromatin and studies have shown using immunoprecipitation technique with anti-HA antibody, that Hho 1 p shows a preferential binding to rDNA sequences. In this project we have tried to confirm the above results in wild type cells, using immunopurifi ed anti rHho 1 p antibody.en_ZA
dc.identifier.apacitationPriya, V. P. (2002). <i>Genomic distribution of histone H1 in budding yeast (Saccharomyces cerevisiae) : yeast chromosome III</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/4324en_ZA
dc.identifier.chicagocitationPriya, Vattem Padma. <i>"Genomic distribution of histone H1 in budding yeast (Saccharomyces cerevisiae) : yeast chromosome III."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2002. http://hdl.handle.net/11427/4324en_ZA
dc.identifier.citationPriya, V. 2002. Genomic distribution of histone H1 in budding yeast (Saccharomyces cerevisiae) : yeast chromosome III. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Priya, Vattem Padma AB - The linker histone HI binds to the nucleosome and is essential for the organization of nucleosomes into the 30-nm filament of the chromatin. This compaction of DNA has a well-characterized effect on DNA function. In Saccharomyces cerevisiae, HHO 1 encodes a putative linker histone with very significant homology to histone HI. In vitro chromatin assembly experiments with recombinant Hho 1 p have shown that it is able to complex with the dinucleosomes in a similar manner to histone HI. It has also been reported that disruption of HHOl has little affect on RNA levels. A longstanding issue concerns the location of Hho 1 p in the chromatin and studies have shown using immunoprecipitation technique with anti-HA antibody, that Hho 1 p shows a preferential binding to rDNA sequences. In this project we have tried to confirm the above results in wild type cells, using immunopurifi ed anti rHho 1 p antibody. DA - 2002 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2002 T1 - Genomic distribution of histone H1 in budding yeast (Saccharomyces cerevisiae) : yeast chromosome III TI - Genomic distribution of histone H1 in budding yeast (Saccharomyces cerevisiae) : yeast chromosome III UR - http://hdl.handle.net/11427/4324 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/4324
dc.identifier.vancouvercitationPriya VP. Genomic distribution of histone H1 in budding yeast (Saccharomyces cerevisiae) : yeast chromosome III. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2002 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/4324en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherCell Biologyen_ZA
dc.titleGenomic distribution of histone H1 in budding yeast (Saccharomyces cerevisiae) : yeast chromosome IIIen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMMeden_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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