Isolation and molecular characterisation of two Pseudomonas sp. ACC deaminase genes

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dc.contributor.advisorThomson, Jennifer Annen_ZA
dc.contributor.advisorBrand Reonen_ZA
dc.contributor.authorCampbell, Bridget Genevieveen_ZA
dc.date.accessioned2014-11-15T19:38:31Z
dc.date.available2014-11-15T19:38:31Z
dc.date.issued1995en_ZA
dc.descriptionBibliography: p. 125-141.en_ZA
dc.description.abstractThe phytohormone ethylene is essential to many plant developmental processes, of which the control of climacteric fruit ripening is among the best characterised. However this hormone eventually causes fruit rotting which results in a non-marketable product. One approach to reduce ethylene synthesis in plants is metabolism of its immediate precursor, 1-aminocyclopropane-1carboxylic acid (ACC). This can be achieved through degradation of ACC by the enzyme ACC deaminase to form α-ketobutyric acid and ammonia. ACC degrading soil microorganisms were identified by their ability to grow on ACC as a sole nitrogen source. Enzyme assays indicated that Pseudomonas had high ACC deaminase gene-specific primers and probes respectively revealed that only one bacterium, Pseudomonas fluerescens strain 17, had a gene with homology to previously sequenced ACC deaminase genes.en_ZA
dc.identifier.apacitationCampbell, B. G. (1995). <i>Isolation and molecular characterisation of two Pseudomonas sp. ACC deaminase genes</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/9650en_ZA
dc.identifier.chicagocitationCampbell, Bridget Genevieve. <i>"Isolation and molecular characterisation of two Pseudomonas sp. ACC deaminase genes."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1995. http://hdl.handle.net/11427/9650en_ZA
dc.identifier.citationCampbell, B. 1995. Isolation and molecular characterisation of two Pseudomonas sp. ACC deaminase genes. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Campbell, Bridget Genevieve AB - The phytohormone ethylene is essential to many plant developmental processes, of which the control of climacteric fruit ripening is among the best characterised. However this hormone eventually causes fruit rotting which results in a non-marketable product. One approach to reduce ethylene synthesis in plants is metabolism of its immediate precursor, 1-aminocyclopropane-1carboxylic acid (ACC). This can be achieved through degradation of ACC by the enzyme ACC deaminase to form α-ketobutyric acid and ammonia. ACC degrading soil microorganisms were identified by their ability to grow on ACC as a sole nitrogen source. Enzyme assays indicated that Pseudomonas had high ACC deaminase gene-specific primers and probes respectively revealed that only one bacterium, Pseudomonas fluerescens strain 17, had a gene with homology to previously sequenced ACC deaminase genes. DA - 1995 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1995 T1 - Isolation and molecular characterisation of two Pseudomonas sp. ACC deaminase genes TI - Isolation and molecular characterisation of two Pseudomonas sp. ACC deaminase genes UR - http://hdl.handle.net/11427/9650 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/9650
dc.identifier.vancouvercitationCampbell BG. Isolation and molecular characterisation of two Pseudomonas sp. ACC deaminase genes. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1995 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/9650en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMicrobiologyen_ZA
dc.titleIsolation and molecular characterisation of two Pseudomonas sp. ACC deaminase genesen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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