Carbon and arsenic metabolism in Thiomonas strains: differences revealed diverse adaptation processes

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dc.contributor.authorBryan, Christopheren_ZA
dc.contributor.authorMarchal, Marieen_ZA
dc.contributor.authorBattaglia-Brunet, Fabienneen_ZA
dc.contributor.authorKugler, Valerieen_ZA
dc.contributor.authorLemaitre-Guillier, Christelleen_ZA
dc.contributor.authorLievremont, Didieren_ZA
dc.contributor.authorBertin, Philippeen_ZA
dc.contributor.authorArsene-Ploetze, Florenceen_ZA
dc.date.accessioned2015-10-30T09:38:29Z
dc.date.available2015-10-30T09:38:29Z
dc.date.issued2009en_ZA
dc.description.abstractBACKGROUND:Thiomonas strains are ubiquitous in arsenic-contaminated environments. Differences between Thiomonas strains in the way they have adapted and respond to arsenic have never been studied in detail. For this purpose, five Thiomonas strains, that are interesting in terms of arsenic metabolism were selected: T. arsenivorans, Thiomonas spp. WJ68 and 3As are able to oxidise As(III), while Thiomonas sp. Ynys1 and T. perometabolis are not. Moreover, T. arsenivorans and 3As present interesting physiological traits, in particular that these strains are able to use As(III) as an electron donor. RESULTS: The metabolism of carbon and arsenic was compared in the five Thiomonas strains belonging to two distinct phylogenetic groups. Greater physiological differences were found between these strains than might have been suggested by 16S rRNA/rpoA gene phylogeny, especially regarding arsenic metabolism. Physiologically, T. perometabolis and Ynys1 were unable to oxidise As(III) and were less arsenic-resistant than the other strains. Genetically, they appeared to lack the aox arsenic-oxidising genes and carried only a single ars arsenic resistance operon. Thiomonas arsenivorans belonged to a distinct phylogenetic group and increased its autotrophic metabolism when arsenic concentration increased. Differential proteomic analysis revealed that in T. arsenivorans, the rbc/cbb genes involved in the assimilation of inorganic carbon were induced in the presence of arsenic, whereas these genes were repressed in Thiomonas sp. 3As. CONCLUSION: Taken together, these results show that these closely related bacteria differ substantially in their response to arsenic, amongst other factors, and suggest different relationships between carbon assimilation and arsenic metabolism.en_ZA
dc.identifier.apacitationBryan, C., Marchal, M., Battaglia-Brunet, F., Kugler, V., Lemaitre-Guillier, C., Lievremont, D., ... Arsene-Ploetze, F. (2009). Carbon and arsenic metabolism in Thiomonas strains: differences revealed diverse adaptation processes. <i>BMC Microbiology</i>, http://hdl.handle.net/11427/14551en_ZA
dc.identifier.chicagocitationBryan, Christopher, Marie Marchal, Fabienne Battaglia-Brunet, Valerie Kugler, Christelle Lemaitre-Guillier, Didier Lievremont, Philippe Bertin, and Florence Arsene-Ploetze "Carbon and arsenic metabolism in Thiomonas strains: differences revealed diverse adaptation processes." <i>BMC Microbiology</i> (2009) http://hdl.handle.net/11427/14551en_ZA
dc.identifier.citationBryan, C. G., Marchal, M., Battaglia-Brunet, F., Kugler, V., Lemaitre-Guillier, C., Lièvremont, D., ... & Arsène-Ploetze, F. (2009). Carbon and arsenic metabolism in Thiomonas strains: differences revealed diverse adaptation processes. BMC microbiology, 9(1), 127.en_ZA
dc.identifier.ris TY - Journal Article AU - Bryan, Christopher AU - Marchal, Marie AU - Battaglia-Brunet, Fabienne AU - Kugler, Valerie AU - Lemaitre-Guillier, Christelle AU - Lievremont, Didier AU - Bertin, Philippe AU - Arsene-Ploetze, Florence AB - BACKGROUND:Thiomonas strains are ubiquitous in arsenic-contaminated environments. Differences between Thiomonas strains in the way they have adapted and respond to arsenic have never been studied in detail. For this purpose, five Thiomonas strains, that are interesting in terms of arsenic metabolism were selected: T. arsenivorans, Thiomonas spp. WJ68 and 3As are able to oxidise As(III), while Thiomonas sp. Ynys1 and T. perometabolis are not. Moreover, T. arsenivorans and 3As present interesting physiological traits, in particular that these strains are able to use As(III) as an electron donor. RESULTS: The metabolism of carbon and arsenic was compared in the five Thiomonas strains belonging to two distinct phylogenetic groups. Greater physiological differences were found between these strains than might have been suggested by 16S rRNA/rpoA gene phylogeny, especially regarding arsenic metabolism. Physiologically, T. perometabolis and Ynys1 were unable to oxidise As(III) and were less arsenic-resistant than the other strains. Genetically, they appeared to lack the aox arsenic-oxidising genes and carried only a single ars arsenic resistance operon. Thiomonas arsenivorans belonged to a distinct phylogenetic group and increased its autotrophic metabolism when arsenic concentration increased. Differential proteomic analysis revealed that in T. arsenivorans, the rbc/cbb genes involved in the assimilation of inorganic carbon were induced in the presence of arsenic, whereas these genes were repressed in Thiomonas sp. 3As. CONCLUSION: Taken together, these results show that these closely related bacteria differ substantially in their response to arsenic, amongst other factors, and suggest different relationships between carbon assimilation and arsenic metabolism. DA - 2009 DB - OpenUCT DO - 10.1186/1471-2180-9-127 DP - University of Cape Town J1 - BMC Microbiology LK - https://open.uct.ac.za PB - University of Cape Town PY - 2009 T1 - Carbon and arsenic metabolism in Thiomonas strains: differences revealed diverse adaptation processes TI - Carbon and arsenic metabolism in Thiomonas strains: differences revealed diverse adaptation processes UR - http://hdl.handle.net/11427/14551 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/14551
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2180-9-127
dc.identifier.vancouvercitationBryan C, Marchal M, Battaglia-Brunet F, Kugler V, Lemaitre-Guillier C, Lievremont D, et al. Carbon and arsenic metabolism in Thiomonas strains: differences revealed diverse adaptation processes. BMC Microbiology. 2009; http://hdl.handle.net/11427/14551.en_ZA
dc.language.isoengen_ZA
dc.publisherBioMed Central Ltden_ZA
dc.publisher.departmentCentre for Bioprocess Engineering Research
dc.publisher.facultyFaculty of Engineering and the Built Environment
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution Licenseen_ZA
dc.rights.holder2009 Bryan et al; licensee BioMed Central Ltd.en_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en_ZA
dc.sourceBMC Microbiologyen_ZA
dc.source.urihttp://www.biomedcentral.com/bmcmicrobiol/en_ZA
dc.subject.otherAdaptation, Physiologicalen_ZA
dc.subject.otherCarbonen_ZA
dc.subject.otherPhylogenyen_ZA
dc.titleCarbon and arsenic metabolism in Thiomonas strains: differences revealed diverse adaptation processesen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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