ROS production and protein oxidation as a novel mechanism for seed dormancy alleviation.

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dc.contributor.authorOracz, Krystyna
dc.contributor.authorBouteau, Hayat El-Maarouf
dc.contributor.authorFarrant, Jill M
dc.contributor.authorCooper, Keren
dc.contributor.authorBelghazi, Maya
dc.contributor.authorJob, Claudette
dc.contributor.authorJob, Dominique
dc.contributor.authorCorbineau, Franc¸oise
dc.contributor.authorBailly, Christophe
dc.date.accessioned2016-07-20T13:03:51Z
dc.date.available2016-07-20T13:03:51Z
dc.date.issued2007
dc.date.updated2016-07-20T13:01:37Z
dc.description.abstractAt harvest, sunflower (Helianthus annuus L.) seeds are dormant and unable to germinate at temperatures below 15C. Seed storage in the dry state, known as after-ripening, is associated with an alleviation of embryonic dormancy allowing subsequent germination at suboptimal temperatures. To identify the process by which dormancy is broken during after-ripening, we focused on the role of reactive oxygen species (ROS) in this phenomenon. After-ripening entailed a progressive accumulation of ROS, namely superoxide anions and hydrogen peroxide, in cells of embryonic axes. This accumulation, which was investigated at the cellular level by electron microscopy, occurred concomitantly with lipid peroxidation and oxidation (carbonylation) of specific embryo proteins. Incubation of dormant seeds for 3 h in the presence of hydrogen cyanide (a compound that breaks dormancy) or methylviologen (a ROS-generating compound) also released dormancy and caused the oxidation of a specific set of embryo proteins. From these observations, we propose a novel mechanism for seed dormancy alleviation. This mechanism involves ROS production and targeted changes in protein carbonylation patterns.en_ZA
dc.identifierhttp://dx.doi.org/10.1111/j.1365-313X.2007.03063.x
dc.identifier.apacitationOracz, K., Bouteau, H. E., Farrant, J. M., Cooper, K., Belghazi, M., Job, C., ... Bailly, C. (2007). ROS production and protein oxidation as a novel mechanism for seed dormancy alleviation. <i>Plant Journal</i>, http://hdl.handle.net/11427/20550en_ZA
dc.identifier.chicagocitationOracz, Krystyna, Hayat El-Maarouf Bouteau, Jill M Farrant, Keren Cooper, Maya Belghazi, Claudette Job, Dominique Job, , and Christophe Bailly "ROS production and protein oxidation as a novel mechanism for seed dormancy alleviation." <i>Plant Journal</i> (2007) http://hdl.handle.net/11427/20550en_ZA
dc.identifier.citationOracz, K., Bouteau, H. E. M., Farrant, J. M., Cooper, K., Belghazi, M., Job, C., ... & Bailly, C. (2007). ROS production and protein oxidation as a novel mechanism for seed dormancy alleviation. The Plant Journal, 50(3), 452-465.en_ZA
dc.identifier.issn0960-7412en_ZA
dc.identifier.ris TY - Journal Article AU - Oracz, Krystyna AU - Bouteau, Hayat El-Maarouf AU - Farrant, Jill M AU - Cooper, Keren AU - Belghazi, Maya AU - Job, Claudette AU - Job, Dominique AU - Corbineau, Franc¸oise AU - Bailly, Christophe AB - At harvest, sunflower (Helianthus annuus L.) seeds are dormant and unable to germinate at temperatures below 15C. Seed storage in the dry state, known as after-ripening, is associated with an alleviation of embryonic dormancy allowing subsequent germination at suboptimal temperatures. To identify the process by which dormancy is broken during after-ripening, we focused on the role of reactive oxygen species (ROS) in this phenomenon. After-ripening entailed a progressive accumulation of ROS, namely superoxide anions and hydrogen peroxide, in cells of embryonic axes. This accumulation, which was investigated at the cellular level by electron microscopy, occurred concomitantly with lipid peroxidation and oxidation (carbonylation) of specific embryo proteins. Incubation of dormant seeds for 3 h in the presence of hydrogen cyanide (a compound that breaks dormancy) or methylviologen (a ROS-generating compound) also released dormancy and caused the oxidation of a specific set of embryo proteins. From these observations, we propose a novel mechanism for seed dormancy alleviation. This mechanism involves ROS production and targeted changes in protein carbonylation patterns. DA - 2007 DB - OpenUCT DP - University of Cape Town J1 - Plant Journal LK - https://open.uct.ac.za PB - University of Cape Town PY - 2007 SM - 0960-7412 T1 - ROS production and protein oxidation as a novel mechanism for seed dormancy alleviation TI - ROS production and protein oxidation as a novel mechanism for seed dormancy alleviation UR - http://hdl.handle.net/11427/20550 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/20550
dc.identifier.urihttp://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2007.03063.x/full
dc.identifier.vancouvercitationOracz K, Bouteau HE, Farrant JM, Cooper K, Belghazi M, Job C, et al. ROS production and protein oxidation as a novel mechanism for seed dormancy alleviation. Plant Journal. 2007; http://hdl.handle.net/11427/20550.en_ZA
dc.languageengen_ZA
dc.publisherWileyen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.sourcePlant Journalen_ZA
dc.source.urihttp://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-313X
dc.subject.otherseed dormancy
dc.subject.otherafter-ripening
dc.subject.otherreactive oxygen species
dc.subject.otherproteome analysis
dc.subject.othercarbonylation
dc.subject.othersunflower
dc.titleROS production and protein oxidation as a novel mechanism for seed dormancy alleviation.en_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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