Membrane shedding in kidney (MDCK) cells as revealed by covalent markers during quantification of endocytosis and transcytosis

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dc.contributor.advisorThilo, Lutzen_ZA
dc.contributor.authorGodenir, Nicoleen_ZA
dc.date.accessioned2018-01-30T14:00:31Z
dc.date.available2018-01-30T14:00:31Z
dc.date.issued1991en_ZA
dc.description.abstractMembrane traffic in polarised cells was investigated by growing Madin-Darby canine kidney (MOCK) cells on ·permeable polycarbonate filter supports which allowed access to both sides of the cell monolayer. Membrane glycoconjugates on the apical and basolateral cell surfaces were labelled enzymatically with ³H- and ¹⁴C-galactose, respectively, to provide covalent membrane markers. Experiments were done to quantitate membrane traffic during endocytosis at the respective plasma membrane domains and that due to transcytosis. Internalized label was quantitatively distinguished from label on the respective cell surface by its resistance to removal by glycosidases.en_ZA
dc.identifier.apacitationGodenir, N. (1991). <i>Membrane shedding in kidney (MDCK) cells as revealed by covalent markers during quantification of endocytosis and transcytosis</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry & Structural Biology. Retrieved from http://hdl.handle.net/11427/27141en_ZA
dc.identifier.chicagocitationGodenir, Nicole. <i>"Membrane shedding in kidney (MDCK) cells as revealed by covalent markers during quantification of endocytosis and transcytosis."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry & Structural Biology, 1991. http://hdl.handle.net/11427/27141en_ZA
dc.identifier.citationGodenir, N. 1991. Membrane shedding in kidney (MDCK) cells as revealed by covalent markers during quantification of endocytosis and transcytosis. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Godenir, Nicole AB - Membrane traffic in polarised cells was investigated by growing Madin-Darby canine kidney (MOCK) cells on ·permeable polycarbonate filter supports which allowed access to both sides of the cell monolayer. Membrane glycoconjugates on the apical and basolateral cell surfaces were labelled enzymatically with ³H- and ¹⁴C-galactose, respectively, to provide covalent membrane markers. Experiments were done to quantitate membrane traffic during endocytosis at the respective plasma membrane domains and that due to transcytosis. Internalized label was quantitatively distinguished from label on the respective cell surface by its resistance to removal by glycosidases. DA - 1991 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1991 T1 - Membrane shedding in kidney (MDCK) cells as revealed by covalent markers during quantification of endocytosis and transcytosis TI - Membrane shedding in kidney (MDCK) cells as revealed by covalent markers during quantification of endocytosis and transcytosis UR - http://hdl.handle.net/11427/27141 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/27141
dc.identifier.vancouvercitationGodenir N. Membrane shedding in kidney (MDCK) cells as revealed by covalent markers during quantification of endocytosis and transcytosis. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry & Structural Biology, 1991 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/27141en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Medical Biochemistry and Structural Biology
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMedical Biochemistryen_ZA
dc.subject.otherBiological transporten_ZA
dc.subject.otherCell membrane permeability.en_ZA
dc.subject.otherEndocytosisen_ZA
dc.subject.otherKidney - citologyen_ZA
dc.subject.otherMembranes - Physiologyen_ZA
dc.titleMembrane shedding in kidney (MDCK) cells as revealed by covalent markers during quantification of endocytosis and transcytosisen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMSc (Med)en_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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