The disruption of Alcaligenes eutrophus by high pressure homogenisation: key factors involved in the process

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dc.contributor.authorHarrison, S T
dc.contributor.authorChase H A
dc.contributor.authorDennis, J S
dc.date.accessioned2016-08-18T11:07:57Z
dc.date.available2016-08-18T11:07:57Z
dc.date.issued1991
dc.date.updated2016-08-16T14:24:20Z
dc.description.abstractThe disruption of the Gram-negative bacterium Alcaligenes eutrophus by high pressure homogenisation, using the APV Gaulin 15M 8BA and 30CD homogenisers is reported. The operating parameters such as operating pressure, number of passes, temperature and biomass concentration, mimicked trends previously reported for yeasts. Extension of the study to consider the effect of cell characteristics, including the growth rate, size and shape, illustrated the dominant effect of the growth phase. The improved disruption of bacterial cultures in the logarithmic phase with respect to stationary phase cultures was confirmed by an increased dependence of actively growing cultures on the operating pressure. An increase in size in excess of 30% on the accumulation of the storage product, PHB in the stationary phase caused little change in the ease of disruption. The use of transmission electron microscopy to directly monitor the disruption on multiple passes shed light on the two-stage nature of this disruption process.en_ZA
dc.identifier.apacitationHarrison, S. T., , & Dennis, J. S. (1991). The disruption of Alcaligenes eutrophus by high pressure homogenisation: key factors involved in the process. <i>Bioseparation</i>, http://hdl.handle.net/11427/21313en_ZA
dc.identifier.chicagocitationHarrison, S T, , and J S Dennis "The disruption of Alcaligenes eutrophus by high pressure homogenisation: key factors involved in the process." <i>Bioseparation</i> (1991) http://hdl.handle.net/11427/21313en_ZA
dc.identifier.citationHarrison, S. T., Chase, H. A., & Dennis, J. S. (1990). The disruption of Alcaligenes eutrophus by high pressure homogenisation: key factors involved in the process. Bioseparation, 2(3), 155-166.en_ZA
dc.identifier.issn0923-179Xen_ZA
dc.identifier.ris TY - Journal Article AU - Harrison, S T AU - Chase H A AU - Dennis, J S AB - The disruption of the Gram-negative bacterium Alcaligenes eutrophus by high pressure homogenisation, using the APV Gaulin 15M 8BA and 30CD homogenisers is reported. The operating parameters such as operating pressure, number of passes, temperature and biomass concentration, mimicked trends previously reported for yeasts. Extension of the study to consider the effect of cell characteristics, including the growth rate, size and shape, illustrated the dominant effect of the growth phase. The improved disruption of bacterial cultures in the logarithmic phase with respect to stationary phase cultures was confirmed by an increased dependence of actively growing cultures on the operating pressure. An increase in size in excess of 30% on the accumulation of the storage product, PHB in the stationary phase caused little change in the ease of disruption. The use of transmission electron microscopy to directly monitor the disruption on multiple passes shed light on the two-stage nature of this disruption process. DA - 1991 DB - OpenUCT DP - University of Cape Town J1 - Bioseparation LK - https://open.uct.ac.za PB - University of Cape Town PY - 1991 SM - 0923-179X T1 - The disruption of Alcaligenes eutrophus by high pressure homogenisation: key factors involved in the process TI - The disruption of Alcaligenes eutrophus by high pressure homogenisation: key factors involved in the process UR - http://hdl.handle.net/11427/21313 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/21313
dc.identifier.vancouvercitationHarrison ST, , Dennis JS. The disruption of Alcaligenes eutrophus by high pressure homogenisation: key factors involved in the process. Bioseparation. 1991; http://hdl.handle.net/11427/21313.en_ZA
dc.languageengen_ZA
dc.publisherSpringer Verlagen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.sourceBioseparationen_ZA
dc.source.urihttp://link.springer.com/journal/10536
dc.titleThe disruption of Alcaligenes eutrophus by high pressure homogenisation: key factors involved in the processen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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