Glucose oxidase induction and the modelling of gluconic acid production using Aspergillus niger

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dc.contributor.advisorHarrison, STLen_ZA
dc.contributor.authorJohnson, Kim Henry Silvanusen_ZA
dc.date.accessioned2016-08-24T13:00:20Z
dc.date.available2016-08-24T13:00:20Z
dc.date.issued1995en_ZA
dc.description.abstractThe aim of this project is to establish and understand the production of glucose oxidase and gluconic acid using the Aspergillus niger bioprocess and predict its response to operating conditions. Glucose oxidase and gluconic acid are produced by a wide range of microbes and have a variety of applications. In this study Aspergillus niger was chosen as the microorganism as it has the "generally accepted as safe" (gras) status in the U.S.A. It is also the major industrial producer. Glucose oxidase catalyses the conversion of glucose, oxygen and water to hydrogen peroxide and gluconic acid. This enzyme is used as a glucose and oxygen scavenger in the food industry and as a diagnostic tool in medicine for glucose determination. Gluconic acid is an organic acid used as a sequestering agent with a broad spectrum of applications. The world market for gluconic acid and its various salts was 45 000 metric tonnes in 1985 (Bigelis cited by Markwell et al. 1989). Gluconic acid and its derivatives can be produced using three technologies: electrolysis, mild chemical oxidation and bioprocess. The first two technologies have not been proven to be comnercially viable. The bioprocess offers diversity of feed and produces other products such as glucose oxidase. Literature has shown that the production of gluconic acid involves two kinetic areas. Firstly, the glucose oxidase enzyme must be induced. Secondly, glucose is converted to gluconic acid by the enzyme glucose oxidase. The factors affecting the kinetics associated with the induction of glucose oxidase have only been described qualitatively. Glucose, oxygen and pH have been shown to affect the induction of glucose oxidase. The effect of pH has been studied by Roukas and Harvey (1989) who found that induction, is maximal at a pH of between 5 and 6. The effect of glucose and oxygen have not been quantified. The kinetics of glucose oxidase conversion of glucose to gluconic acid have been well described by Atkinson and Lester (1974).en_ZA
dc.identifier.apacitationJohnson, K. H. S. (1995). <i>Glucose oxidase induction and the modelling of gluconic acid production using Aspergillus niger</i>. (Thesis). University of Cape Town ,Faculty of Engineering & the Built Environment ,Centre for Bioprocess Engineering Research. Retrieved from http://hdl.handle.net/11427/21517en_ZA
dc.identifier.chicagocitationJohnson, Kim Henry Silvanus. <i>"Glucose oxidase induction and the modelling of gluconic acid production using Aspergillus niger."</i> Thesis., University of Cape Town ,Faculty of Engineering & the Built Environment ,Centre for Bioprocess Engineering Research, 1995. http://hdl.handle.net/11427/21517en_ZA
dc.identifier.citationJohnson, K. 1995. Glucose oxidase induction and the modelling of gluconic acid production using Aspergillus niger. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Johnson, Kim Henry Silvanus AB - The aim of this project is to establish and understand the production of glucose oxidase and gluconic acid using the Aspergillus niger bioprocess and predict its response to operating conditions. Glucose oxidase and gluconic acid are produced by a wide range of microbes and have a variety of applications. In this study Aspergillus niger was chosen as the microorganism as it has the "generally accepted as safe" (gras) status in the U.S.A. It is also the major industrial producer. Glucose oxidase catalyses the conversion of glucose, oxygen and water to hydrogen peroxide and gluconic acid. This enzyme is used as a glucose and oxygen scavenger in the food industry and as a diagnostic tool in medicine for glucose determination. Gluconic acid is an organic acid used as a sequestering agent with a broad spectrum of applications. The world market for gluconic acid and its various salts was 45 000 metric tonnes in 1985 (Bigelis cited by Markwell et al. 1989). Gluconic acid and its derivatives can be produced using three technologies: electrolysis, mild chemical oxidation and bioprocess. The first two technologies have not been proven to be comnercially viable. The bioprocess offers diversity of feed and produces other products such as glucose oxidase. Literature has shown that the production of gluconic acid involves two kinetic areas. Firstly, the glucose oxidase enzyme must be induced. Secondly, glucose is converted to gluconic acid by the enzyme glucose oxidase. The factors affecting the kinetics associated with the induction of glucose oxidase have only been described qualitatively. Glucose, oxygen and pH have been shown to affect the induction of glucose oxidase. The effect of pH has been studied by Roukas and Harvey (1989) who found that induction, is maximal at a pH of between 5 and 6. The effect of glucose and oxygen have not been quantified. The kinetics of glucose oxidase conversion of glucose to gluconic acid have been well described by Atkinson and Lester (1974). DA - 1995 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1995 T1 - Glucose oxidase induction and the modelling of gluconic acid production using Aspergillus niger TI - Glucose oxidase induction and the modelling of gluconic acid production using Aspergillus niger UR - http://hdl.handle.net/11427/21517 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/21517
dc.identifier.vancouvercitationJohnson KHS. Glucose oxidase induction and the modelling of gluconic acid production using Aspergillus niger. [Thesis]. University of Cape Town ,Faculty of Engineering & the Built Environment ,Centre for Bioprocess Engineering Research, 1995 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/21517en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentCentre for Bioprocess Engineering Research
dc.publisher.facultyFaculty of Engineering and the Built Environment
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherBioprocess Engineeringen_ZA
dc.titleGlucose oxidase induction and the modelling of gluconic acid production using Aspergillus nigeren_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMSc (Eng)en_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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