Increased resistance to biotrophic pathogens in the Arabidopsis constitutive induced resistance 1 mutant is EDS1 and PAD4-dependent and modulated by environmental temperature

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dc.contributor.authorCarstens, Marykeen_ZA
dc.contributor.authorMcCrindle, Tyronne Ken_ZA
dc.contributor.authorAdams, Nicoletteen_ZA
dc.contributor.authorDiener, Anastashiaen_ZA
dc.contributor.authorGuzha, Delroy Ten_ZA
dc.contributor.authorMurray, Shane Len_ZA
dc.contributor.authorParker, Jane Een_ZA
dc.contributor.authorDenby, Katherine Jen_ZA
dc.contributor.authorIngle, Robert Aen_ZA
dc.date.accessioned2015-11-23T12:32:12Z
dc.date.available2015-11-23T12:32:12Z
dc.date.issued2014en_ZA
dc.description.abstractThe Arabidopsis constitutive induced resistance 1 ( cir1 ) mutant displays salicylic acid (SA)-dependent constitutive expression of defence genes and enhanced resistance to biotrophic pathogens. To further characterise the role of CIR1 in plant immunity we conducted epistasis analyses with two key components of the SA-signalling branch of the defence network, ENHANCED DISEASE SUSCEPTIBILITY1 (EDS1) and PHYTOALEXIN DEFICIENT4 (PAD4). We demonstrate that the constitutive defence phenotypes of cir1 require both EDS1 and PAD4, indicating that CIR1 lies upstream of the EDS1-PAD4 regulatory node in the immune signalling network. In light of this finding we examined EDS1 expression in cir1 and observed increased protein, but not mRNA levels in this mutant, suggesting that CIR1 might act as a negative regulator of EDS1 via a post-transcriptional mechanism. Finally, as environmental temperature is known to influence the outcome of plant-pathogen interactions, we analysed cir1 plants grown at 18, 22 or 25°C. We found that susceptibility to Pseudomonas syringae pv. tomato ( Pst ) DC3000 is modulated by temperature in cir1 . Greatest resistance to this pathogen (relative to PR-1:LUC control plants) was observed at 18°C, while at 25°C no difference in susceptibility between cir1 and control plants was apparent. The increase in resistance to Pst DC3000 at 18°C correlated with a stunted growth phenotype, suggesting that activation of defence responses may be enhanced at lower temperatures in the cir1 mutant.en_ZA
dc.identifier.apacitationCarstens, M., McCrindle, T. K., Adams, N., Diener, A., Guzha, D. T., Murray, S. L., ... Ingle, R. A. (2014). Increased resistance to biotrophic pathogens in the Arabidopsis constitutive induced resistance 1 mutant is EDS1 and PAD4-dependent and modulated by environmental temperature. <i>PLoS One</i>, http://hdl.handle.net/11427/15314en_ZA
dc.identifier.chicagocitationCarstens, Maryke, Tyronne K McCrindle, Nicolette Adams, Anastashia Diener, Delroy T Guzha, Shane L Murray, Jane E Parker, Katherine J Denby, and Robert A Ingle "Increased resistance to biotrophic pathogens in the Arabidopsis constitutive induced resistance 1 mutant is EDS1 and PAD4-dependent and modulated by environmental temperature." <i>PLoS One</i> (2014) http://hdl.handle.net/11427/15314en_ZA
dc.identifier.citationCarstens, M., McCrindle, T. K., Adams, N., Diener, A., Guzha, D. T., Murray, S. L., ... & Ingle, R. A. (2013). Increased resistance to biotrophic pathogens in the Arabidopsis constitutive induced resistance 1 mutant is EDS1 and PAD4-dependent and modulated by environmental temperature. PloS one, 9(10), e109853. doi:10.1371/journal.pone.0109853en_ZA
dc.identifier.ris TY - Journal Article AU - Carstens, Maryke AU - McCrindle, Tyronne K AU - Adams, Nicolette AU - Diener, Anastashia AU - Guzha, Delroy T AU - Murray, Shane L AU - Parker, Jane E AU - Denby, Katherine J AU - Ingle, Robert A AB - The Arabidopsis constitutive induced resistance 1 ( cir1 ) mutant displays salicylic acid (SA)-dependent constitutive expression of defence genes and enhanced resistance to biotrophic pathogens. To further characterise the role of CIR1 in plant immunity we conducted epistasis analyses with two key components of the SA-signalling branch of the defence network, ENHANCED DISEASE SUSCEPTIBILITY1 (EDS1) and PHYTOALEXIN DEFICIENT4 (PAD4). We demonstrate that the constitutive defence phenotypes of cir1 require both EDS1 and PAD4, indicating that CIR1 lies upstream of the EDS1-PAD4 regulatory node in the immune signalling network. In light of this finding we examined EDS1 expression in cir1 and observed increased protein, but not mRNA levels in this mutant, suggesting that CIR1 might act as a negative regulator of EDS1 via a post-transcriptional mechanism. Finally, as environmental temperature is known to influence the outcome of plant-pathogen interactions, we analysed cir1 plants grown at 18, 22 or 25°C. We found that susceptibility to Pseudomonas syringae pv. tomato ( Pst ) DC3000 is modulated by temperature in cir1 . Greatest resistance to this pathogen (relative to PR-1:LUC control plants) was observed at 18°C, while at 25°C no difference in susceptibility between cir1 and control plants was apparent. The increase in resistance to Pst DC3000 at 18°C correlated with a stunted growth phenotype, suggesting that activation of defence responses may be enhanced at lower temperatures in the cir1 mutant. DA - 2014 DB - OpenUCT DO - 10.1371/journal.pone.0109853 DP - University of Cape Town J1 - PLoS One LK - https://open.uct.ac.za PB - University of Cape Town PY - 2014 T1 - Increased resistance to biotrophic pathogens in the Arabidopsis constitutive induced resistance 1 mutant is EDS1 and PAD4-dependent and modulated by environmental temperature TI - Increased resistance to biotrophic pathogens in the Arabidopsis constitutive induced resistance 1 mutant is EDS1 and PAD4-dependent and modulated by environmental temperature UR - http://hdl.handle.net/11427/15314 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/15314
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pone.0109853
dc.identifier.vancouvercitationCarstens M, McCrindle TK, Adams N, Diener A, Guzha DT, Murray SL, et al. Increased resistance to biotrophic pathogens in the Arabidopsis constitutive induced resistance 1 mutant is EDS1 and PAD4-dependent and modulated by environmental temperature. PLoS One. 2014; http://hdl.handle.net/11427/15314.en_ZA
dc.language.isoengen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_ZA
dc.rights.holder© 2014 Carstens et alen_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/4.0en_ZA
dc.sourcePLoS Oneen_ZA
dc.source.urihttp://journals.plos.org/plosoneen_ZA
dc.subject.otherGene expressionen_ZA
dc.subject.otherPlant pathogensen_ZA
dc.subject.otherBiotrophic pathogensen_ZA
dc.subject.otherPhenotypesen_ZA
dc.subject.otherRegulator genesen_ZA
dc.subject.otherSignaling networksen_ZA
dc.subject.otherInnate immune systemen_ZA
dc.subject.otherPolymerase chain reactionen_ZA
dc.titleIncreased resistance to biotrophic pathogens in the Arabidopsis constitutive induced resistance 1 mutant is EDS1 and PAD4-dependent and modulated by environmental temperatureen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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