Identification of Antimycobacterial Natural Products from a Library of Marine Invertebrate Extracts

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dc.contributor.authorAcquah, Kojo Sekyi
dc.contributor.authorBeukes, Denzil R
dc.contributor.authorSeldon, Ronnett
dc.contributor.authorJordaan, Audrey
dc.contributor.authorSunassee, Suthananda N
dc.contributor.authorWarner, Digby F
dc.contributor.authorGammon, David W
dc.date.accessioned2022-04-09T16:52:37Z
dc.date.available2022-04-09T16:52:37Z
dc.date.issued2022-01-28
dc.date.updated2022-02-24T14:50:15Z
dc.description.abstractTuberculosis (TB) remains a public health crisis, requiring the urgent identification of new anti-mycobacterial drugs. We screened several organic and aqueous marine invertebrate extracts for their in vitro inhibitory activity against the causative organism, Mycobacterium tuberculosis. Here, we report the results obtained for 54 marine invertebrate extracts. The chemical components of two of the extracts were dereplicated, using 1H NMR and HR-LCMS with GNPS molecular networking, and these extracts were further subjected to an activity-guided isolation process to purify the bioactive components. Hyrtios reticulatus yielded heteronemin 1 and Jaspis splendens was found to produce the bengamide class of compounds, of which bengamides P 2 and Q 3 were isolated, while a new derivative, bengamide S 5, was putatively identified and its structure predicted, based on the similarity of its MS/MS fragmentation pattern to those of other bengamides. The isolated bioactive metabolites and semi-pure fractions exhibited M. tuberculosis growth inhibitory activity, in the rangeen_US
dc.identifierdoi: 10.3390/medicines9020009
dc.identifier.apacitationAcquah, K. S., Beukes, D. R., Seldon, R., Jordaan, A., Sunassee, S. N., Warner, D. F., & Gammon, D. W. (2022). Identification of Antimycobacterial Natural Products from a Library of Marine Invertebrate Extracts. <i>Medicines</i>, 9(2), 9. http://hdl.handle.net/11427/36318en_ZA
dc.identifier.chicagocitationAcquah, Kojo Sekyi, Denzil R Beukes, Ronnett Seldon, Audrey Jordaan, Suthananda N Sunassee, Digby F Warner, and David W Gammon "Identification of Antimycobacterial Natural Products from a Library of Marine Invertebrate Extracts." <i>Medicines</i> 9, 2. (2022): 9. http://hdl.handle.net/11427/36318en_ZA
dc.identifier.citationAcquah, K.S., Beukes, D.R., Seldon, R., Jordaan, A., Sunassee, S.N., Warner, D.F. & Gammon, D.W. 2022. Identification of Antimycobacterial Natural Products from a Library of Marine Invertebrate Extracts. <i>Medicines.</i> 9(2):9. http://hdl.handle.net/11427/36318en_ZA
dc.identifier.ris TY - Journal Article AU - Acquah, Kojo Sekyi AU - Beukes, Denzil R AU - Seldon, Ronnett AU - Jordaan, Audrey AU - Sunassee, Suthananda N AU - Warner, Digby F AU - Gammon, David W AB - Tuberculosis (TB) remains a public health crisis, requiring the urgent identification of new anti-mycobacterial drugs. We screened several organic and aqueous marine invertebrate extracts for their in vitro inhibitory activity against the causative organism, Mycobacterium tuberculosis. Here, we report the results obtained for 54 marine invertebrate extracts. The chemical components of two of the extracts were dereplicated, using 1H NMR and HR-LCMS with GNPS molecular networking, and these extracts were further subjected to an activity-guided isolation process to purify the bioactive components. Hyrtios reticulatus yielded heteronemin 1 and Jaspis splendens was found to produce the bengamide class of compounds, of which bengamides P 2 and Q 3 were isolated, while a new derivative, bengamide S 5, was putatively identified and its structure predicted, based on the similarity of its MS/MS fragmentation pattern to those of other bengamides. The isolated bioactive metabolites and semi-pure fractions exhibited M. tuberculosis growth inhibitory activity, in the range DA - 2022-01-28 DB - OpenUCT DP - University of Cape Town IS - 2 J1 - Medicines LK - https://open.uct.ac.za PY - 2022 T1 - Identification of Antimycobacterial Natural Products from a Library of Marine Invertebrate Extracts TI - Identification of Antimycobacterial Natural Products from a Library of Marine Invertebrate Extracts UR - http://hdl.handle.net/11427/36318 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/36318
dc.identifier.vancouvercitationAcquah KS, Beukes DR, Seldon R, Jordaan A, Sunassee SN, Warner DF, et al. Identification of Antimycobacterial Natural Products from a Library of Marine Invertebrate Extracts. Medicines. 2022;9(2):9. http://hdl.handle.net/11427/36318.en_ZA
dc.language.isoenen_US
dc.publisher.departmentDepartment of Chemistryen_US
dc.publisher.facultyFaculty of Scienceen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.sourceMedicinesen_US
dc.source.journalissue2en_US
dc.source.journalvolume9en_US
dc.source.pagination9en_US
dc.source.urihttps://www.mdpi.com/journal/medicines
dc.titleIdentification of Antimycobacterial Natural Products from a Library of Marine Invertebrate Extractsen_US
dc.typeJournal Articleen_US
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