Efficiency of Buccal DNA sampling device in the mortuary

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dc.contributor.authorTredoux, Simone
dc.contributor.authorMfolozi, Sipho
dc.contributor.authorShires, Karen
dc.date.accessioned2015-10-09T11:14:32Z
dc.date.available2015-10-09T11:14:32Z
dc.date.issued2015-08-31
dc.description.abstractIdentification of forensic DNA samples by short tandem repeat (STR) profiling is currently an essential component of criminal investigations and can aid in linking perpetrators to crimes as well as identifying missing individuals or unidentified remains. In South Africa, recent amendments to legislation have allowed for the mandatory acquisition of reference DNA samples from certain offenders in order to populate the new National Forensic DNA Database. A novel method for the collection of buccal samples, the EasiCollect device, has been proposed to facilitate the collection of these DNA samples, replacing blood collecting devices as the standard method of DNA collection. Subsequently, this device has been introduced into South African state mortuaries to assist in the identification of deceased individuals. In order to ascertain if this device is suitable for use in the postmortem setting, an investigation was performed to compare the main methodology currently utilised within South African mortuaries, namely femoral blood transferred to ‘Fast Technology for Analysis of nucleic acids’ (FTA) cards, and buccal cells obtained using the EasiCollect device. DNA yields and STR genotyping results were compared between the two collection methods in thirty deceased individuals. Buccal samples provided a significantly greater DNA yield than blood samples, while no significant difference was observed between the qualities of the sample types as measured by the 260/280 nm ratio. Full STR profiles were obtained from all blood and buccal samples, with amplification efficiency showing limited DNA degradation and PCR inhibition in these samples, and only 3% of samples giving potentially disputable results. Numerous issues surrounding the collection of blood samples, however, indicated that this method is not optimal for use in the mortuary, with the EasiCollect device providing a more practical and robust method for the collection of DNA samples in the mortuaryen_ZA
dc.identifier.apacitationTredoux, S., Mfolozi, S., & Shires, K. (2015). Efficiency of Buccal DNA sampling device in the mortuary. <i>Journal of Forensic Investigation</i>, http://hdl.handle.net/11427/14157en_ZA
dc.identifier.chicagocitationTredoux, Simone, Sipho Mfolozi, and Karen Shires "Efficiency of Buccal DNA sampling device in the mortuary." <i>Journal of Forensic Investigation</i> (2015) http://hdl.handle.net/11427/14157en_ZA
dc.identifier.citationTredouw, S.; Mfolozi, S. & Shires, K. (2015). Efficiency of Buccal DNA Sampling Device in the Mortuary. Journal of Forensic Investigation, 3(2), 6en_ZA
dc.identifier.issn2330-0396en_ZA
dc.identifier.ris TY - Journal Article AU - Tredoux, Simone AU - Mfolozi, Sipho AU - Shires, Karen AB - Identification of forensic DNA samples by short tandem repeat (STR) profiling is currently an essential component of criminal investigations and can aid in linking perpetrators to crimes as well as identifying missing individuals or unidentified remains. In South Africa, recent amendments to legislation have allowed for the mandatory acquisition of reference DNA samples from certain offenders in order to populate the new National Forensic DNA Database. A novel method for the collection of buccal samples, the EasiCollect device, has been proposed to facilitate the collection of these DNA samples, replacing blood collecting devices as the standard method of DNA collection. Subsequently, this device has been introduced into South African state mortuaries to assist in the identification of deceased individuals. In order to ascertain if this device is suitable for use in the postmortem setting, an investigation was performed to compare the main methodology currently utilised within South African mortuaries, namely femoral blood transferred to ‘Fast Technology for Analysis of nucleic acids’ (FTA) cards, and buccal cells obtained using the EasiCollect device. DNA yields and STR genotyping results were compared between the two collection methods in thirty deceased individuals. Buccal samples provided a significantly greater DNA yield than blood samples, while no significant difference was observed between the qualities of the sample types as measured by the 260/280 nm ratio. Full STR profiles were obtained from all blood and buccal samples, with amplification efficiency showing limited DNA degradation and PCR inhibition in these samples, and only 3% of samples giving potentially disputable results. Numerous issues surrounding the collection of blood samples, however, indicated that this method is not optimal for use in the mortuary, with the EasiCollect device providing a more practical and robust method for the collection of DNA samples in the mortuary DA - 2015-08-31 DB - OpenUCT DP - University of Cape Town J1 - Journal of Forensic Investigation LK - https://open.uct.ac.za PB - University of Cape Town PY - 2015 SM - 2330-0396 T1 - Efficiency of Buccal DNA sampling device in the mortuary TI - Efficiency of Buccal DNA sampling device in the mortuary UR - http://hdl.handle.net/11427/14157 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/14157
dc.identifier.vancouvercitationTredoux S, Mfolozi S, Shires K. Efficiency of Buccal DNA sampling device in the mortuary. Journal of Forensic Investigation. 2015; http://hdl.handle.net/11427/14157.en_ZA
dc.languageengen_ZA
dc.publisherAvens Publishing Groupen_ZA
dc.publisher.departmentDivision of Haematologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsCreative Commons Attribution 4.0 International (CC BY 4.0)*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_ZA
dc.sourceJournal of Forensic Investigationen_ZA
dc.source.urihttp://www.avensonline.org/medical/forensic-investigation/home-14/
dc.titleEfficiency of Buccal DNA sampling device in the mortuaryen_ZA
dc.typeJournal Articleen_ZA
uct.subject.keywordsEasiCollecten_ZA
uct.subject.keywordsSTR typingen_ZA
uct.subject.keywordsBuccal cellsen_ZA
uct.subject.keywordsBlooden_ZA
uct.subject.keywordsDecedentsen_ZA
uct.subject.keywordsDNA extractionen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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