Novel and newer nucleic acid amplification tests for the diagnosis of TB

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dc.contributor.advisorDheda, Keertanen_ZA
dc.contributor.advisorTheron, Granten_ZA
dc.contributor.authorMatinyenya, Brianen_ZA
dc.date.accessioned2016-07-25T11:25:25Z
dc.date.available2016-07-25T11:25:25Z
dc.date.issued2016en_ZA
dc.description.abstractBackground: Current tools for TB diagnosis have suboptimal accuracy, perform poorly in diagnosing extra-pulmonary TB, and are not point of care; hence results have a slow turn-around time. Objective: This project evaluated the diagnostic accuracy of the promising novel loop mediated isothermal amplification (LAMP) assay on sputum, and that of the semi-automated Xpert MTB/RIF (Xpert) test on non-sputum specimens (bronchoalveolar lavage fluid [BALF], tracheal aspirates, and cerebrospinal fluid [CSF]) from South African patients with suspected TB (the accuracy of Xpert using these fluids was unknown at the time this work was performed). Methodology: Biological samples (sputum, tracheal aspirates, BALF, or CSF) were collected from patients with suspected TB. Liquid culture served as the reference standard for the diagnosis of definite TB. Accuracy was evaluated according to HIV and smear microscopy status, where appropriate. The relationship between test performance and bacterial load (culture time-to-positivity [TTP]) was also compared. For the evaluation of LAMP, 2 spot sputa of approximately 4 ml were collected from 301 patients (60 μl of sputum was used for the assay). For the evaluation of Xpert on BALF, 152 patients who were sputum scarce or smear-negative were recruited (1 ml of the BALF aliquot or a re-suspended pellet from 10 ml BALF was used). For the evaluation of Xpert on tracheal aspirates, 120 tracheal aspirates from patients enrolled in the intensive care unit (ICU) were tested. For the evaluation of Xpert on CSF, 235 patients with suspected TBM had a lumbar puncture with 1 ml of CSF or where available a re-suspended pellet from 3 ml of CSF evaluated using Xpert.en_ZA
dc.identifier.apacitationMatinyenya, B. (2016). <i>Novel and newer nucleic acid amplification tests for the diagnosis of TB</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Pulmonology. Retrieved from http://hdl.handle.net/11427/20680en_ZA
dc.identifier.chicagocitationMatinyenya, Brian. <i>"Novel and newer nucleic acid amplification tests for the diagnosis of TB."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Pulmonology, 2016. http://hdl.handle.net/11427/20680en_ZA
dc.identifier.citationMatinyenya, B. 2016. Novel and newer nucleic acid amplification tests for the diagnosis of TB. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Matinyenya, Brian AB - Background: Current tools for TB diagnosis have suboptimal accuracy, perform poorly in diagnosing extra-pulmonary TB, and are not point of care; hence results have a slow turn-around time. Objective: This project evaluated the diagnostic accuracy of the promising novel loop mediated isothermal amplification (LAMP) assay on sputum, and that of the semi-automated Xpert MTB/RIF (Xpert) test on non-sputum specimens (bronchoalveolar lavage fluid [BALF], tracheal aspirates, and cerebrospinal fluid [CSF]) from South African patients with suspected TB (the accuracy of Xpert using these fluids was unknown at the time this work was performed). Methodology: Biological samples (sputum, tracheal aspirates, BALF, or CSF) were collected from patients with suspected TB. Liquid culture served as the reference standard for the diagnosis of definite TB. Accuracy was evaluated according to HIV and smear microscopy status, where appropriate. The relationship between test performance and bacterial load (culture time-to-positivity [TTP]) was also compared. For the evaluation of LAMP, 2 spot sputa of approximately 4 ml were collected from 301 patients (60 μl of sputum was used for the assay). For the evaluation of Xpert on BALF, 152 patients who were sputum scarce or smear-negative were recruited (1 ml of the BALF aliquot or a re-suspended pellet from 10 ml BALF was used). For the evaluation of Xpert on tracheal aspirates, 120 tracheal aspirates from patients enrolled in the intensive care unit (ICU) were tested. For the evaluation of Xpert on CSF, 235 patients with suspected TBM had a lumbar puncture with 1 ml of CSF or where available a re-suspended pellet from 3 ml of CSF evaluated using Xpert. DA - 2016 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2016 T1 - Novel and newer nucleic acid amplification tests for the diagnosis of TB TI - Novel and newer nucleic acid amplification tests for the diagnosis of TB UR - http://hdl.handle.net/11427/20680 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/20680
dc.identifier.vancouvercitationMatinyenya B. Novel and newer nucleic acid amplification tests for the diagnosis of TB. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Pulmonology, 2016 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/20680en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Pulmonologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherPulmonologyen_ZA
dc.titleNovel and newer nucleic acid amplification tests for the diagnosis of TBen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMSc (Med)en_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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