A novel stress-inducible antioxidant enzyme identified from the resurrection plant Xerophyta viscosa Baker.

dc.contributor.authorMowla, Shaheen B
dc.contributor.authorThomson, Jennifer A
dc.contributor.authorFarrant, Jill
dc.contributor.authorMundree, Sagadevan G
dc.date.accessioned2016-08-04T09:29:51Z
dc.date.available2016-08-04T09:29:51Z
dc.date.issued2002
dc.date.updated2016-08-04T08:26:13Z
dc.description.abstractA cDNA corresponding to 1-Cys peroxiredoxin, an evolutionarily conserved thiol-specific antioxidant enzyme, was isolated from Xerophyta viscosa Baker, a resurrection plant indigenous to Southern Africa and belonging to the family Velloziaceae. The cDNA, designated XvPer1, contains an open reading frame that encodes a polypeptide of 219 residues with a predicted molecular weight of 24.2 kDa. The XvPer1 polypeptide shows significant sequence identity (approx. 70%) to other recently identified plant 1-Cys peroxiredoxins and relatively high levels of sequence similarity (approx. 40%) to non-plant 1-Cys peroxiredoxins. The XvPer1 cDNA contains a putative polyadenylation site. As for all 1-Cys peroxiredoxins identified to date, the amino acid sequence proposed to constitute the active site of the enzyme, PVCTTE, is highly conserved in XvPer1. It also contains a putative bipartite nuclear localization signal. Southern blot analysis revealed that there is a single copy of XvPer1 in the X. viscosa genome. All angiosperm 1-Cys peroxiredoxins described to date are seed-specific and absent in vegetative tissues even under stress conditions; therefore, XvPer1 is unique in that it is expressed in the vegetative tissues of X. viscosa. The XvPer1 transcript was absent in fully hydrated X. viscosa tissue but levels increased in tissues subjected to abiotic stresses such as dehydration, heat (42 °C), high light intensity (1,500 µmol photons m–2 s–1) and when treated with abscisic acid (100 µM ABA) and sodium chloride (100 mM NaCl). Western blot analyses correlated with the patterns of expression of XvPer1 transcripts under different stress conditions. Immunofluorescence analyses revealed that XvPer1 is localized in the nucleus of dehydrated X. viscosa leaf cells. These results suggest that XvPer1 is a stress-inducible gene, which may function to protect nucleic acids within the nucleus against oxidative injury.en_ZA
dc.identifierhttp://dx.doi.org/10.1007/s00425-002-0819-0
dc.identifier.apacitationMowla, S. B., Thomson, J. A., Farrant, J., & Mundree, S. G. (2002). A novel stress-inducible antioxidant enzyme identified from the resurrection plant Xerophyta viscosa Baker. <i>Planta</i>, http://hdl.handle.net/11427/21111en_ZA
dc.identifier.chicagocitationMowla, Shaheen B, Jennifer A Thomson, Jill Farrant, and Sagadevan G Mundree "A novel stress-inducible antioxidant enzyme identified from the resurrection plant Xerophyta viscosa Baker." <i>Planta</i> (2002) http://hdl.handle.net/11427/21111en_ZA
dc.identifier.citationMowla, S. B., Thomson, J. A., Farrant, J. M., & Mundree, S. G. (2002). A novel stress-inducible antioxidant enzyme identified from the resurrection plant Xerophyta viscosa Baker. Planta, 215(5), 716-726.en_ZA
dc.identifier.issn0032-0935en_ZA
dc.identifier.ris TY - Journal Article AU - Mowla, Shaheen B AU - Thomson, Jennifer A AU - Farrant, Jill AU - Mundree, Sagadevan G AB - A cDNA corresponding to 1-Cys peroxiredoxin, an evolutionarily conserved thiol-specific antioxidant enzyme, was isolated from Xerophyta viscosa Baker, a resurrection plant indigenous to Southern Africa and belonging to the family Velloziaceae. The cDNA, designated XvPer1, contains an open reading frame that encodes a polypeptide of 219 residues with a predicted molecular weight of 24.2 kDa. The XvPer1 polypeptide shows significant sequence identity (approx. 70%) to other recently identified plant 1-Cys peroxiredoxins and relatively high levels of sequence similarity (approx. 40%) to non-plant 1-Cys peroxiredoxins. The XvPer1 cDNA contains a putative polyadenylation site. As for all 1-Cys peroxiredoxins identified to date, the amino acid sequence proposed to constitute the active site of the enzyme, PVCTTE, is highly conserved in XvPer1. It also contains a putative bipartite nuclear localization signal. Southern blot analysis revealed that there is a single copy of XvPer1 in the X. viscosa genome. All angiosperm 1-Cys peroxiredoxins described to date are seed-specific and absent in vegetative tissues even under stress conditions; therefore, XvPer1 is unique in that it is expressed in the vegetative tissues of X. viscosa. The XvPer1 transcript was absent in fully hydrated X. viscosa tissue but levels increased in tissues subjected to abiotic stresses such as dehydration, heat (42 °C), high light intensity (1,500 µmol photons m–2 s–1) and when treated with abscisic acid (100 µM ABA) and sodium chloride (100 mM NaCl). Western blot analyses correlated with the patterns of expression of XvPer1 transcripts under different stress conditions. Immunofluorescence analyses revealed that XvPer1 is localized in the nucleus of dehydrated X. viscosa leaf cells. These results suggest that XvPer1 is a stress-inducible gene, which may function to protect nucleic acids within the nucleus against oxidative injury. DA - 2002 DB - OpenUCT DP - University of Cape Town J1 - Planta LK - https://open.uct.ac.za PB - University of Cape Town PY - 2002 SM - 0032-0935 T1 - A novel stress-inducible antioxidant enzyme identified from the resurrection plant Xerophyta viscosa Baker TI - A novel stress-inducible antioxidant enzyme identified from the resurrection plant Xerophyta viscosa Baker UR - http://hdl.handle.net/11427/21111 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/21111
dc.identifier.vancouvercitationMowla SB, Thomson JA, Farrant J, Mundree SG. A novel stress-inducible antioxidant enzyme identified from the resurrection plant Xerophyta viscosa Baker. Planta. 2002; http://hdl.handle.net/11427/21111.en_ZA
dc.languageengen_ZA
dc.publisherSpringeren_ZA
dc.publisher.institutionUniversity of Cape Town
dc.sourcePlantaen_ZA
dc.source.urihttp://link.springer.com/journal/425
dc.subject.otherDesiccation stress
dc.subject.otherPeroxiredoxin
dc.subject.otherResurrection plant
dc.subject.otherXerophyta
dc.titleA novel stress-inducible antioxidant enzyme identified from the resurrection plant Xerophyta viscosa Baker.en_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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