Molecular characterisation of virus in the brains of patients with measles inclusion body encephalitis (MIBE)

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dc.contributor.authorHardie, Dianaen_ZA
dc.contributor.authorAlbertyn, Christineen_ZA
dc.contributor.authorHeckmann, Jeannineen_ZA
dc.contributor.authorSmuts, Heidien_ZA
dc.date.accessioned2015-11-27T09:27:27Z
dc.date.available2015-11-27T09:27:27Z
dc.date.issued2013en_ZA
dc.description.abstractBACKGROUND: During 2009/10 a major measles epidemic caused by genotype B3 occurred in South Africa. Measles inclusion body encephalitis (MIBE) was diagnosed in a number of highly immuno-compromised HIV patients. The diagnosis was based on typical clinical and MRI findings and positive measles virus PCR in brain or CSF.To characterize the brain virus, nucleoprotein, matrix, fusion and haemagglutinin genes from 4 cases was compared with virus from acutely infected patients. METHODS: cDNA was synthesized using random primers and viral genes were amplified by nested RT-PCR. PCR products were sequenced in the forward and reverse direction and a contig of each gene was created. Sequences were aligned with reference sequences from GenBank and other local sequences. RESULTS: Brain virus was very similar to the South African epidemic virus. Features characteristic of persistent measles virus in the brain were absent. Mutation frequency in brain virus was similar to epidemic virus and had the same substitution preference (U to C and C to U). The virus of 2 patients had the same L454W mutation in the fusion protein. CONCLUSION: The brain virus was very similar to the epidemic strain. The relatively few mutations probably reflect the short time from infection to brain disease in these highly immuno-compromised patients.en_ZA
dc.identifier.apacitationHardie, D., Albertyn, C., Heckmann, J., & Smuts, H. (2013). Molecular characterisation of virus in the brains of patients with measles inclusion body encephalitis (MIBE). <i>Virology Journal</i>, http://hdl.handle.net/11427/15373en_ZA
dc.identifier.chicagocitationHardie, Diana, Christine Albertyn, Jeannine Heckmann, and Heidi Smuts "Molecular characterisation of virus in the brains of patients with measles inclusion body encephalitis (MIBE)." <i>Virology Journal</i> (2013) http://hdl.handle.net/11427/15373en_ZA
dc.identifier.citationHardie, D. R., Albertyn, C., Heckmann, J. M., & Smuts, H. E. (2013). Molecular characterisation of virus in the brains of patients with measles inclusion body encephalitis (MIBE). Virol J, 10(1), 283.en_ZA
dc.identifier.ris TY - Journal Article AU - Hardie, Diana AU - Albertyn, Christine AU - Heckmann, Jeannine AU - Smuts, Heidi AB - BACKGROUND: During 2009/10 a major measles epidemic caused by genotype B3 occurred in South Africa. Measles inclusion body encephalitis (MIBE) was diagnosed in a number of highly immuno-compromised HIV patients. The diagnosis was based on typical clinical and MRI findings and positive measles virus PCR in brain or CSF.To characterize the brain virus, nucleoprotein, matrix, fusion and haemagglutinin genes from 4 cases was compared with virus from acutely infected patients. METHODS: cDNA was synthesized using random primers and viral genes were amplified by nested RT-PCR. PCR products were sequenced in the forward and reverse direction and a contig of each gene was created. Sequences were aligned with reference sequences from GenBank and other local sequences. RESULTS: Brain virus was very similar to the South African epidemic virus. Features characteristic of persistent measles virus in the brain were absent. Mutation frequency in brain virus was similar to epidemic virus and had the same substitution preference (U to C and C to U). The virus of 2 patients had the same L454W mutation in the fusion protein. CONCLUSION: The brain virus was very similar to the epidemic strain. The relatively few mutations probably reflect the short time from infection to brain disease in these highly immuno-compromised patients. DA - 2013 DB - OpenUCT DO - 10.1186/1743-422X-10-283 DP - University of Cape Town J1 - Virology Journal LK - https://open.uct.ac.za PB - University of Cape Town PY - 2013 T1 - Molecular characterisation of virus in the brains of patients with measles inclusion body encephalitis (MIBE) TI - Molecular characterisation of virus in the brains of patients with measles inclusion body encephalitis (MIBE) UR - http://hdl.handle.net/11427/15373 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/15373
dc.identifier.urihttp://dx.doi.org/10.1186/1743-422X-10-283
dc.identifier.vancouvercitationHardie D, Albertyn C, Heckmann J, Smuts H. Molecular characterisation of virus in the brains of patients with measles inclusion body encephalitis (MIBE). Virology Journal. 2013; http://hdl.handle.net/11427/15373.en_ZA
dc.language.isoengen_ZA
dc.publisherBioMed Central Ltden_ZA
dc.publisher.departmentDivision of Virologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution Licenseen_ZA
dc.rights.holderHardie et al.; licensee BioMed Central Ltd. 2013en_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en_ZA
dc.sourceVirology Journalen_ZA
dc.source.urihttp://virologyj.biomedcentral.com/en_ZA
dc.subject.otherMeasles inclusion body encephalitisen_ZA
dc.subject.otherMIBEen_ZA
dc.subject.otherSubacute measles encephalitisen_ZA
dc.subject.otherNeuro-virulenceen_ZA
dc.subject.otherMutationen_ZA
dc.subject.otherImmuno-compromiseden_ZA
dc.subject.otherHuman immunodeficiency virusen_ZA
dc.titleMolecular characterisation of virus in the brains of patients with measles inclusion body encephalitis (MIBE)en_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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