Interactions between selected metal compounds and marine heterotrophic bacteria

Master Thesis

1984

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University of Cape Town

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A method was developed to test the toxicity of several metal compounds to selected bacteria. The technique was based on the agar diffusion method. Reliability and reproducibility were enhanced by careful standardisation of experimental parameters. By quantification of the concentration of metal compounds in the media using sequential strips of agar it was .possible to prepare standard graphs (metal concentration vs. distance from disc). These graphs demonstrated the exponential rate of diffusion of metals. The diffusion rates were metal specific. In the case of lead compounds the diffusion rate was poor. A media was developed which allowed lead chloride to diffuse relatively well.
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Bibliography: pages 122-131.

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