A cost effective RFLP method to genotype Solute carrier organic anion 1B1 (SLCO1B1) c.1929A>C (p.Leu643Phe, rs34671512); a variant with potential effect on rosuvastatin pharmacokinetics

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dc.contributor.authorSoko, Nyarai D
dc.contributor.authorMasimirembwa, Collen
dc.contributor.authorDandara, Collet
dc.date.accessioned2018-07-11T09:36:22Z
dc.date.available2018-07-11T09:36:22Z
dc.date.issued2018-06-14
dc.date.updated2018-06-17T03:36:09Z
dc.description.abstractObjective: This study describes a restriction fragment polymorphism protocol for rapidly screening the polymorphism SLCO1B1 c.1929A>C in genomic DNA samples. The polymorphism SLCO1B1 c.1929A>C has been associated with increased activity resulting in increased hepatic uptake of drugs. Currently SLCO1B1 c.1929A>C is genotyped using direct sequencing techniques and 5′ nuclease based assays which can be cost prohibiting in resource limited settings. The aim of this study therefore was to design and validate a cost effective RFLP for genotyping the SLCO1B1 c.1929A>C polymorphism. This study was designed to investigate the effect of the polymorphism SLCO1B1 c.1929A>C on interindividual variability in rosuvastatin pharmacokinetics in healthy volunteers of African descent. Results We describe a restriction fragment length polymorphism method to genotype SLCO1B1 c.1929A>C polymorphism using the restriction enzyme Ase1. A student’s t test with Welch correction was used to establish association between the SLCO1B1 c.1929A>C variant and rosuvastatin exposure. The frequency of the SLCO1B1 c.1929C allele amongst Zimbabweans was 6%. The SLCO1B1 c.1929C allele was associated with a 75% reduction (P < 0.001) in rosuvastatin exposure when compared to individuals carrying the wild type SLCO1B1 c.1929A allele. Polymorphism c.1929A>C may therefore play a significant role in rosuvastatin response. The RFLP method is quick and cost effective.
dc.identifier.apacitationSoko, N. D., Masimirembwa, C., & Dandara, C. (2018). A cost effective RFLP method to genotype Solute carrier organic anion 1B1 (SLCO1B1) c.1929A>C (p.Leu643Phe, rs34671512); a variant with potential effect on rosuvastatin pharmacokinetics. http://hdl.handle.net/11427/28290en_ZA
dc.identifier.chicagocitationSoko, Nyarai D, Collen Masimirembwa, and Collet Dandara "A cost effective RFLP method to genotype Solute carrier organic anion 1B1 (SLCO1B1) c.1929A>C (p.Leu643Phe, rs34671512); a variant with potential effect on rosuvastatin pharmacokinetics." (2018) http://hdl.handle.net/11427/28290en_ZA
dc.identifier.citationSoko, N. D., Masimirembwa, C., & Dandara, C. (2018). A cost effective RFLP method to genotype Solute carrier organic anion 1B1 (SLCO1B1) c. 1929A> C (p. Leu643Phe, rs34671512); a variant with potential effect on rosuvastatin pharmacokinetics. BMC research notes, 11(1), 384.
dc.identifier.ris TY - Journal Article AU - Soko, Nyarai D AU - Masimirembwa, Collen AU - Dandara, Collet AB - Objective: This study describes a restriction fragment polymorphism protocol for rapidly screening the polymorphism SLCO1B1 c.1929A>C in genomic DNA samples. The polymorphism SLCO1B1 c.1929A>C has been associated with increased activity resulting in increased hepatic uptake of drugs. Currently SLCO1B1 c.1929A>C is genotyped using direct sequencing techniques and 5′ nuclease based assays which can be cost prohibiting in resource limited settings. The aim of this study therefore was to design and validate a cost effective RFLP for genotyping the SLCO1B1 c.1929A>C polymorphism. This study was designed to investigate the effect of the polymorphism SLCO1B1 c.1929A>C on interindividual variability in rosuvastatin pharmacokinetics in healthy volunteers of African descent. Results We describe a restriction fragment length polymorphism method to genotype SLCO1B1 c.1929A>C polymorphism using the restriction enzyme Ase1. A student’s t test with Welch correction was used to establish association between the SLCO1B1 c.1929A>C variant and rosuvastatin exposure. The frequency of the SLCO1B1 c.1929C allele amongst Zimbabweans was 6%. The SLCO1B1 c.1929C allele was associated with a 75% reduction (P < 0.001) in rosuvastatin exposure when compared to individuals carrying the wild type SLCO1B1 c.1929A allele. Polymorphism c.1929A>C may therefore play a significant role in rosuvastatin response. The RFLP method is quick and cost effective. DA - 2018-06-14 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2018 T1 - A cost effective RFLP method to genotype Solute carrier organic anion 1B1 (SLCO1B1) c.1929A>C (p.Leu643Phe, rs34671512); a variant with potential effect on rosuvastatin pharmacokinetics TI - A cost effective RFLP method to genotype Solute carrier organic anion 1B1 (SLCO1B1) c.1929A>C (p.Leu643Phe, rs34671512); a variant with potential effect on rosuvastatin pharmacokinetics UR - http://hdl.handle.net/11427/28290 ER - en_ZA
dc.identifier.urihttps://doi.org/10.1186/s13104-018-3469-4
dc.identifier.urihttp://hdl.handle.net/11427/28290
dc.identifier.vancouvercitationSoko ND, Masimirembwa C, Dandara C. A cost effective RFLP method to genotype Solute carrier organic anion 1B1 (SLCO1B1) c.1929A>C (p.Leu643Phe, rs34671512); a variant with potential effect on rosuvastatin pharmacokinetics. 2018; http://hdl.handle.net/11427/28290.en_ZA
dc.language.isoen
dc.publisherBioMed Central
dc.publisher.departmentDivision of Human Geneticsen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rights.holderThe Author(s)
dc.subject.otherSLCO1B1
dc.subject.otherRosuvastatin
dc.subject.otherAfrican
dc.subject.otherrs34671512
dc.subject.otherRFLP
dc.subject.otherPharmacogenetics
dc.subject.otherPharmacokinetics
dc.subject.otherSLCO1B1*22
dc.subject.otherSLCO1B1*35
dc.titleA cost effective RFLP method to genotype Solute carrier organic anion 1B1 (SLCO1B1) c.1929A>C (p.Leu643Phe, rs34671512); a variant with potential effect on rosuvastatin pharmacokinetics
dc.typeJournal Article
uct.type.filetypeText
uct.type.filetypeImage
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