Detection of natural infection with Mycobacterium intracellulare in healthy wild-caught Chacma baboons (Papio ursinus) by ESAT-6 and CFP-10 IFN-gamma ELISPOT tests following a tuberculosis outbreak

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dc.contributor.authorChege, Geralden_ZA
dc.contributor.authorWarren, Robinen_ZA
dc.contributor.authorvan Pittius, Nicoen_ZA
dc.contributor.authorBurgers, Wendyen_ZA
dc.contributor.authorWilkinson, Roberten_ZA
dc.contributor.authorShephard, Eniden_ZA
dc.contributor.authorWilliamson, Anna-Liseen_ZA
dc.date.accessioned2015-10-28T07:05:25Z
dc.date.available2015-10-28T07:05:25Z
dc.date.issued2008en_ZA
dc.description.abstractBACKGROUND:Both tuberculous and non-tuberculous mycobacteria can cause infection in nonhuman primates (NHP), indicating the existence of potential zoonotic transmission between these animals and visitors to zoos or animal handlers in primate facilities. Screening of mycobacterial infections in NHP is traditionally done by tuberculin skin test (TST), which is unable to distinguish between pathogenic and non-pathogenic mycobacterial infections. In this study, we investigated the use of ESAT-6 and CFP-10 for detection of mycobacterial infections in a wild-caught baboon colony after one baboon died of tuberculosis (TB). METHODS: Peripheral blood lymphocytes for interferon-gamma enzyme-linked immunospot assay (IFN-gamma ELISPOT) assay were obtained from TST positive baboons and those in contact with tuberculous baboons before being euthanased, autopsied and lung tissues taken for histology and mycobacterial culture. RESULTS: Both ESAT-6 and CFP-10 IFN-gamma ELISPOT assays were able to detect early M. tuberculosis but also M. intracellulare infection. Although this indicates potential cross-reactivity with M. intracellulare antigens, the method was able to distinguish M. bovis BCG vaccination from M. tuberculosis infection. This assay performed better than the TST, which failed to detect one M. tuberculosis and two early M. intracellulare infections. CONCLUSION: These results suggest that the IFN-gamma ELISPOT assay could improve the detection of M tuberculosis infections when screening NHP. There is some doubt, however, concerning specificity, as the assay scored positive three animals infected with M. intracellulare.en_ZA
dc.identifier.apacitationChege, G., Warren, R., van Pittius, N., Burgers, W., Wilkinson, R., Shephard, E., & Williamson, A. (2008). Detection of natural infection with Mycobacterium intracellulare in healthy wild-caught Chacma baboons (Papio ursinus) by ESAT-6 and CFP-10 IFN-gamma ELISPOT tests following a tuberculosis outbreak. <i>BMC Microbiology</i>, http://hdl.handle.net/11427/14470en_ZA
dc.identifier.chicagocitationChege, Gerald, Robin Warren, Nico van Pittius, Wendy Burgers, Robert Wilkinson, Enid Shephard, and Anna-Lise Williamson "Detection of natural infection with Mycobacterium intracellulare in healthy wild-caught Chacma baboons (Papio ursinus) by ESAT-6 and CFP-10 IFN-gamma ELISPOT tests following a tuberculosis outbreak." <i>BMC Microbiology</i> (2008) http://hdl.handle.net/11427/14470en_ZA
dc.identifier.citationChege, G. K., Warren, R. M., van Pittius, N. C., Burgers, W. A., Wilkinson, R. J., Shephard, E. G., & Williamson, A. L. (2008). Detection of natural infection with Mycobacterium intracellulare in healthy wild-caught Chacma baboons (Papio ursinus) by ESAT-6 and CFP-10 IFN-γ ELISPOT tests following a tuberculosis outbreak. BMC microbiology, 8(1), 27.en_ZA
dc.identifier.ris TY - Journal Article AU - Chege, Gerald AU - Warren, Robin AU - van Pittius, Nico AU - Burgers, Wendy AU - Wilkinson, Robert AU - Shephard, Enid AU - Williamson, Anna-Lise AB - BACKGROUND:Both tuberculous and non-tuberculous mycobacteria can cause infection in nonhuman primates (NHP), indicating the existence of potential zoonotic transmission between these animals and visitors to zoos or animal handlers in primate facilities. Screening of mycobacterial infections in NHP is traditionally done by tuberculin skin test (TST), which is unable to distinguish between pathogenic and non-pathogenic mycobacterial infections. In this study, we investigated the use of ESAT-6 and CFP-10 for detection of mycobacterial infections in a wild-caught baboon colony after one baboon died of tuberculosis (TB). METHODS: Peripheral blood lymphocytes for interferon-gamma enzyme-linked immunospot assay (IFN-gamma ELISPOT) assay were obtained from TST positive baboons and those in contact with tuberculous baboons before being euthanased, autopsied and lung tissues taken for histology and mycobacterial culture. RESULTS: Both ESAT-6 and CFP-10 IFN-gamma ELISPOT assays were able to detect early M. tuberculosis but also M. intracellulare infection. Although this indicates potential cross-reactivity with M. intracellulare antigens, the method was able to distinguish M. bovis BCG vaccination from M. tuberculosis infection. This assay performed better than the TST, which failed to detect one M. tuberculosis and two early M. intracellulare infections. CONCLUSION: These results suggest that the IFN-gamma ELISPOT assay could improve the detection of M tuberculosis infections when screening NHP. There is some doubt, however, concerning specificity, as the assay scored positive three animals infected with M. intracellulare. DA - 2008 DB - OpenUCT DO - 10.1186/1471-2180-8-27 DP - University of Cape Town J1 - BMC Microbiology LK - https://open.uct.ac.za PB - University of Cape Town PY - 2008 T1 - Detection of natural infection with Mycobacterium intracellulare in healthy wild-caught Chacma baboons (Papio ursinus) by ESAT-6 and CFP-10 IFN-gamma ELISPOT tests following a tuberculosis outbreak TI - Detection of natural infection with Mycobacterium intracellulare in healthy wild-caught Chacma baboons (Papio ursinus) by ESAT-6 and CFP-10 IFN-gamma ELISPOT tests following a tuberculosis outbreak UR - http://hdl.handle.net/11427/14470 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/14470
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2180-8-27
dc.identifier.vancouvercitationChege G, Warren R, van Pittius N, Burgers W, Wilkinson R, Shephard E, et al. Detection of natural infection with Mycobacterium intracellulare in healthy wild-caught Chacma baboons (Papio ursinus) by ESAT-6 and CFP-10 IFN-gamma ELISPOT tests following a tuberculosis outbreak. BMC Microbiology. 2008; http://hdl.handle.net/11427/14470.en_ZA
dc.language.isoengen_ZA
dc.publisherBioMed Central Ltden_ZA
dc.publisher.departmentDepartment of Clinical Laboratory Sciencesen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution Licenseen_ZA
dc.rights.holder2008 Chege et al; licensee BioMed Central Ltd.en_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en_ZA
dc.sourceBMC Microbiologyen_ZA
dc.source.urihttp://www.biomedcentral.com/bmcmicrobiol/en_ZA
dc.subject.otherMycobacterium intracellulareen_ZA
dc.subject.otherChacma baboonsen_ZA
dc.titleDetection of natural infection with Mycobacterium intracellulare in healthy wild-caught Chacma baboons (Papio ursinus) by ESAT-6 and CFP-10 IFN-gamma ELISPOT tests following a tuberculosis outbreaken_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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