A Study to investigate the role of p27 and Cyclin E immunoexpression as a prognostic factor in early breast carcinoma

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dc.contributor.authorPillay, Komalaen_ZA
dc.contributor.authorMcCleod, Heatheren_ZA
dc.contributor.authorChetty, Runjanen_ZA
dc.contributor.authorHall, Paulineen_ZA
dc.date.accessioned2015-11-11T12:00:33Z
dc.date.available2015-11-11T12:00:33Z
dc.date.issued2011en_ZA
dc.description.abstractBACKGROUND: Cyclin E and p27 expression is easy to assess in human tissues by standard immunohistochemical techniques. Immunohistochemistry is cost effective, relatively easy to perform and will play more of a role in the future management of cancer. The aim of this study was to investigate the role of p27 and cyclin E immunoexpression as a prognostic factor in early breast carcinoma. METHODS: Cyclin E and p27 immunohistochemistry was performed on sixty six cases of breast carcinoma submitted over a five year period to the Division of Anatomical Pathology, Groote Schuur hospital; Whittaker and Associates; and PathCare. All tumours included in this study were less than 5 cm in diameter (pT1 and pT2 stage) and all the patients had wide local excisions performed. Follow up information was obtained from patient folders in the Department of Radiation Oncology. RESULTS: There was no significant association of cyclin E and p27 expression with distant metastasis free survival (MFS) for all invasive carcinomas in contrast to grade, lymph node spread and vascular invasion. However, there was a statistically significant direct association of cyclin E with distant metastases in all invasive carcinomas, in the subgroup of infiltrating duct carcinomas (IDC) and in the node negative group when cyclin E was stratified as negative and positive (low/high). In this study of early breast carcinoma, only 9/66 cases showed cyclin E expression. Of these, four patients had distant metastases, one patient had a local recurrence and four patients were alive at last follow-up. Furthermore, cyclin E expression was significantly associated with grade, lymph node spread, oestrogen receptor status and histological type. None of the lobular carcinomas showed cyclin E positivity and only one case of lobular carcinoma presented with distant metastases.59/66 cases were positive (low/high) for p27 while seven cases were negative, 22 cases showed low expression and 37 cases demonstrated high p27 expression.p27 was significantly associated with oestrogen receptor status only for all invasive carcinomas and in the IDC group. There was no statistical relationship between p27 and cyclin E, but 50 (76%) tumours with positive p27 expression were negative for cyclin E. There were similar results for the invasive ductal carcinoma subgroup. CONCLUSION: This study shows that p27 and cyclin E are not good independent prognostic markers for early breast carcinoma in contrast to grade, lymph node spread and vascular invasion for all invasive carcinomas. However, cyclin E provides some prognostic value as there is a direct statistical association with the development of distant metastases. Many previous studies have correlated overexpression of cyclin E with an aggressive course. The inverse relationship between p27 and cyclin E expression which has been reported in the literature has been highlighted, but this was not statistically significant. Most cases showed positive p27 expression and negative Cyclin E expression. This may be due to the early stage of the disease.en_ZA
dc.identifier.apacitationPillay, K., McCleod, H., Chetty, R., & Hall, P. (2011). A Study to investigate the role of p27 and Cyclin E immunoexpression as a prognostic factor in early breast carcinoma. <i>World Journal of Surgical Oncology</i>, http://hdl.handle.net/11427/14885en_ZA
dc.identifier.chicagocitationPillay, Komala, Heather McCleod, Runjan Chetty, and Pauline Hall "A Study to investigate the role of p27 and Cyclin E immunoexpression as a prognostic factor in early breast carcinoma." <i>World Journal of Surgical Oncology</i> (2011) http://hdl.handle.net/11427/14885en_ZA
dc.identifier.citationPillay, K., McCleod, H., Chetty, R., & Hall, P. (2011). A Study to investigate the role of p27 and Cyclin E immunoexpression as a prognostic factor in early breast carcinoma. World Journal of Surgical Oncology, 9, 31.en_ZA
dc.identifier.ris TY - Journal Article AU - Pillay, Komala AU - McCleod, Heather AU - Chetty, Runjan AU - Hall, Pauline AB - BACKGROUND: Cyclin E and p27 expression is easy to assess in human tissues by standard immunohistochemical techniques. Immunohistochemistry is cost effective, relatively easy to perform and will play more of a role in the future management of cancer. The aim of this study was to investigate the role of p27 and cyclin E immunoexpression as a prognostic factor in early breast carcinoma. METHODS: Cyclin E and p27 immunohistochemistry was performed on sixty six cases of breast carcinoma submitted over a five year period to the Division of Anatomical Pathology, Groote Schuur hospital; Whittaker and Associates; and PathCare. All tumours included in this study were less than 5 cm in diameter (pT1 and pT2 stage) and all the patients had wide local excisions performed. Follow up information was obtained from patient folders in the Department of Radiation Oncology. RESULTS: There was no significant association of cyclin E and p27 expression with distant metastasis free survival (MFS) for all invasive carcinomas in contrast to grade, lymph node spread and vascular invasion. However, there was a statistically significant direct association of cyclin E with distant metastases in all invasive carcinomas, in the subgroup of infiltrating duct carcinomas (IDC) and in the node negative group when cyclin E was stratified as negative and positive (low/high). In this study of early breast carcinoma, only 9/66 cases showed cyclin E expression. Of these, four patients had distant metastases, one patient had a local recurrence and four patients were alive at last follow-up. Furthermore, cyclin E expression was significantly associated with grade, lymph node spread, oestrogen receptor status and histological type. None of the lobular carcinomas showed cyclin E positivity and only one case of lobular carcinoma presented with distant metastases.59/66 cases were positive (low/high) for p27 while seven cases were negative, 22 cases showed low expression and 37 cases demonstrated high p27 expression.p27 was significantly associated with oestrogen receptor status only for all invasive carcinomas and in the IDC group. There was no statistical relationship between p27 and cyclin E, but 50 (76%) tumours with positive p27 expression were negative for cyclin E. There were similar results for the invasive ductal carcinoma subgroup. CONCLUSION: This study shows that p27 and cyclin E are not good independent prognostic markers for early breast carcinoma in contrast to grade, lymph node spread and vascular invasion for all invasive carcinomas. However, cyclin E provides some prognostic value as there is a direct statistical association with the development of distant metastases. Many previous studies have correlated overexpression of cyclin E with an aggressive course. The inverse relationship between p27 and cyclin E expression which has been reported in the literature has been highlighted, but this was not statistically significant. Most cases showed positive p27 expression and negative Cyclin E expression. This may be due to the early stage of the disease. DA - 2011 DB - OpenUCT DO - 10.1186/1477-7819-9-31 DP - University of Cape Town J1 - World Journal of Surgical Oncology LK - https://open.uct.ac.za PB - University of Cape Town PY - 2011 T1 - A Study to investigate the role of p27 and Cyclin E immunoexpression as a prognostic factor in early breast carcinoma TI - A Study to investigate the role of p27 and Cyclin E immunoexpression as a prognostic factor in early breast carcinoma UR - http://hdl.handle.net/11427/14885 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/14885
dc.identifier.urihttp://dx.doi.org/10.1186/1477-7819-9-31
dc.identifier.vancouvercitationPillay K, McCleod H, Chetty R, Hall P. A Study to investigate the role of p27 and Cyclin E immunoexpression as a prognostic factor in early breast carcinoma. World Journal of Surgical Oncology. 2011; http://hdl.handle.net/11427/14885.en_ZA
dc.language.isoengen_ZA
dc.publisherBioMed Central Ltden_ZA
dc.publisher.departmentDivision of Anatomical Pathologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution Licenseen_ZA
dc.rights.holder2011 Pillay et al; licensee BioMed Central Ltd.en_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en_ZA
dc.sourceWorld Journal of Surgical Oncologyen_ZA
dc.source.urihttp://wjso.biomedcentral.com/en_ZA
dc.subject.otherp27en_ZA
dc.subject.othercyclin Een_ZA
dc.subject.otherearly breast carcinomaen_ZA
dc.subject.otherImmunohistochemistryen_ZA
dc.titleA Study to investigate the role of p27 and Cyclin E immunoexpression as a prognostic factor in early breast carcinomaen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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