Characterization of a thermostable amidase and development of a bioreactor process for lactic acid production

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dc.contributor.advisorBurton, Stephanie Gailen_ZA
dc.contributor.advisorCowan, DAen_ZA
dc.contributor.authorMakhongela, Happy Stevenen_ZA
dc.date.accessioned2014-08-28T09:15:47Z
dc.date.available2014-08-28T09:15:47Z
dc.date.issued2005en_ZA
dc.description.abstractThis thesis reports studies of the biochemical properties of a newly isolated thermostable amidase from Bacillus sp. RAPc8, and development of a continuous reactor process for the production of a target product. The amidase was cloned and over-expressed in E. coli BL21 strain pNH 223 pLySs by Cameron (2001). Earlier work by Cameron (2001) on the production and purification of the recombinant amidase showed that the enzyme could be produced with high levels of expression in shake-flask culture. Furthermore, preliminary studies have also shown that the molecular weight of the amidase was approximately 35kDa, and that it acts optimally at a temperature and pH of 50°C and 7.2 respectively.en_ZA
dc.identifier.apacitationMakhongela, H. S. (2005). <i>Characterization of a thermostable amidase and development of a bioreactor process for lactic acid production</i>. (Thesis). University of Cape Town ,Faculty of Engineering & the Built Environment ,Department of Chemical Engineering. Retrieved from http://hdl.handle.net/11427/6692en_ZA
dc.identifier.chicagocitationMakhongela, Happy Steven. <i>"Characterization of a thermostable amidase and development of a bioreactor process for lactic acid production."</i> Thesis., University of Cape Town ,Faculty of Engineering & the Built Environment ,Department of Chemical Engineering, 2005. http://hdl.handle.net/11427/6692en_ZA
dc.identifier.citationMakhongela, H. 2005. Characterization of a thermostable amidase and development of a bioreactor process for lactic acid production. University of Cape Town.en_ZA
dc.identifier.risTY - Thesis / Dissertation AU - Makhongela, Happy Steven AB - This thesis reports studies of the biochemical properties of a newly isolated thermostable amidase from Bacillus sp. RAPc8, and development of a continuous reactor process for the production of a target product. The amidase was cloned and over-expressed in E. coli BL21 strain pNH 223 pLySs by Cameron (2001). Earlier work by Cameron (2001) on the production and purification of the recombinant amidase showed that the enzyme could be produced with high levels of expression in shake-flask culture. Furthermore, preliminary studies have also shown that the molecular weight of the amidase was approximately 35kDa, and that it acts optimally at a temperature and pH of 50°C and 7.2 respectively. DA - 2005 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2005 T1 - Characterization of a thermostable amidase and development of a bioreactor process for lactic acid production TI - Characterization of a thermostable amidase and development of a bioreactor process for lactic acid production UR - http://hdl.handle.net/11427/6692 ER -en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/6692
dc.identifier.vancouvercitationMakhongela HS. Characterization of a thermostable amidase and development of a bioreactor process for lactic acid production. [Thesis]. University of Cape Town ,Faculty of Engineering & the Built Environment ,Department of Chemical Engineering, 2005 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/6692en_ZA
dc.language.isoeng
dc.publisher.departmentCentre for Bioprocess Engineering Researchen_ZA
dc.publisher.facultyFaculty of Engineering and the Built Environment
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherBioprocess Engineeringen_ZA
dc.titleCharacterization of a thermostable amidase and development of a bioreactor process for lactic acid productionen_ZA
dc.typeThesis
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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