Phylogeographic analysis reveals strong geographical structuring in the klipspringer, Oreotragus oreotragus

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dc.contributor.advisorO'Ryan, Colleenen_ZA
dc.contributor.authorLe Roex, Nikkien_ZA
dc.date.accessioned2016-04-20T14:11:19Z
dc.date.available2016-04-20T14:11:19Z
dc.date.issued2008en_ZA
dc.description.abstractThe klipspringer, Oreotragus oreotragus, occurs from the Western Cape of South Africa through to north-east Africa. Anthropological factors such as hunting have eradicated the klipspringer in parts of its former range and efforts have begun to reintroduce klipspringer back into these areas. This has highlighted the need for the proper understanding of the genetic variation, phylogeographic and population structure of the species, as well as the genetic validity of the 11 subspecies designations. Mitochondrial control region and cytochrome b sequence data were used to investigate these topics using blood, faecal and museum specimens. Cytochrome b and control region fragments were amplified in 83 and 60 samples respectively, out of a total sample set of 89 individuals. The generation of authentic mitochondrial DNA control region fragments proved difficult, with both Numt and PCR recombinant sequences identified after amplification. High levels of genetic variation were observed in the klipspringer, with cytochrome b and control region haplotype diversities of 0.78 and 0.98 respectively. Phylogenetic and network analyses showed the distinct geographical clustering of individuals into two major groups, south/south-western (S/SW) and east/north-eastern (E/NE), with the S/SW further split into two divergent groups. This suggests that the two S/SW groups were separated and isolated as a result of shifting climatic conditions in the late Pl iocene/early Pleistocene, after which secondary contact occurred and klipspringer moved upwards from southern Africa to colonise the east. The results from this study have conservation implications with respect to translocation policies, suggesting that translocations should only occur within the geographic groups identified in order to avoid outbreeding depression.en_ZA
dc.identifier.apacitationLe Roex, N. (2008). <i>Phylogeographic analysis reveals strong geographical structuring in the klipspringer, Oreotragus oreotragus</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/19029en_ZA
dc.identifier.chicagocitationLe Roex, Nikki. <i>"Phylogeographic analysis reveals strong geographical structuring in the klipspringer, Oreotragus oreotragus."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2008. http://hdl.handle.net/11427/19029en_ZA
dc.identifier.citationLe Roex, N. 2008. Phylogeographic analysis reveals strong geographical structuring in the klipspringer, Oreotragus oreotragus. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Le Roex, Nikki AB - The klipspringer, Oreotragus oreotragus, occurs from the Western Cape of South Africa through to north-east Africa. Anthropological factors such as hunting have eradicated the klipspringer in parts of its former range and efforts have begun to reintroduce klipspringer back into these areas. This has highlighted the need for the proper understanding of the genetic variation, phylogeographic and population structure of the species, as well as the genetic validity of the 11 subspecies designations. Mitochondrial control region and cytochrome b sequence data were used to investigate these topics using blood, faecal and museum specimens. Cytochrome b and control region fragments were amplified in 83 and 60 samples respectively, out of a total sample set of 89 individuals. The generation of authentic mitochondrial DNA control region fragments proved difficult, with both Numt and PCR recombinant sequences identified after amplification. High levels of genetic variation were observed in the klipspringer, with cytochrome b and control region haplotype diversities of 0.78 and 0.98 respectively. Phylogenetic and network analyses showed the distinct geographical clustering of individuals into two major groups, south/south-western (S/SW) and east/north-eastern (E/NE), with the S/SW further split into two divergent groups. This suggests that the two S/SW groups were separated and isolated as a result of shifting climatic conditions in the late Pl iocene/early Pleistocene, after which secondary contact occurred and klipspringer moved upwards from southern Africa to colonise the east. The results from this study have conservation implications with respect to translocation policies, suggesting that translocations should only occur within the geographic groups identified in order to avoid outbreeding depression. DA - 2008 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2008 T1 - Phylogeographic analysis reveals strong geographical structuring in the klipspringer, Oreotragus oreotragus TI - Phylogeographic analysis reveals strong geographical structuring in the klipspringer, Oreotragus oreotragus UR - http://hdl.handle.net/11427/19029 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/19029
dc.identifier.vancouvercitationLe Roex N. Phylogeographic analysis reveals strong geographical structuring in the klipspringer, Oreotragus oreotragus. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2008 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/19029en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMolecular and Cell Biologyen_ZA
dc.titlePhylogeographic analysis reveals strong geographical structuring in the klipspringer, Oreotragus oreotragusen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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