Influence of the Viral Superoxide Dismutase (SOD) Homologue on Lumpy Skin Disease Virus (LSDV) Growth, Histopathology and Pathogenicity

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dc.contributor.authorDouglass, Nicola
dc.contributor.authorMunyanduki, Henry
dc.contributor.authorOmar, Ruzaiq
dc.contributor.authorGers, Sophette
dc.contributor.authorMutowembwa, Paidamwoyo
dc.contributor.authorHeath, Livio
dc.contributor.authorWilliamson, Anna-Lise
dc.date.accessioned2021-10-13T17:29:17Z
dc.date.available2021-10-13T17:29:17Z
dc.date.issued2020-11-07
dc.date.updated2020-12-24T18:46:26Z
dc.description.abstractLumpy skin disease is an important economic disease of cattle that is controlled by vaccination. This paper presents an investigation into the role of the lumpy skin disease virus (LSDV) superoxide dismutase (SOD) homologue on growth and histopathology of the virus both in vitro and in vivo. SOD homologue knock-out and knock-in recombinants (nLSDV∆SOD-UCT and nLSDVSODis-UCT, respectively) were constructed and compared to the Neethling vaccine (nLSDV) for growth in a permissive bovine cell line as well as on fertilized chick chorioallantoic membranes (CAMs). The infected CAMs were scored for histological changes. Deletion of the SOD homologue from LSDV reduced virus growth both in Madin-Darby bovine kidney (MDBK) cells as well as on CAMs. Furthermore, the knockout virus showed reduced inflammation in CAMs and more ballooning degeneration. A pilot experiment was performed in cattle to compare the lesions produced by the different LSDV constructs in the same animal. One animal developed a larger lesion to nLSDV∆SOD-UCT compared to both nLSDVSODis-UCT and nLSDV. Histological analysis of biopsies of these lesions shows less inflammation and necrosis associated with nLSDVSODis-UCT compared to nLSDV and nLSDV∆SOD-UCT. None of the vaccinated animals showed disseminated LSDV disease, indicating that the candidate vaccines are safe for further testing. Our results suggest that the SOD homologue may improve immunogenicity and reduce virulence.en_US
dc.identifierdoi: 10.3390/vaccines8040664
dc.identifier.apacitationDouglass, N., Munyanduki, H., Omar, R., Gers, S., Mutowembwa, P., Heath, L., & Williamson, A. (2020). Influence of the Viral Superoxide Dismutase (SOD) Homologue on Lumpy Skin Disease Virus (LSDV) Growth, Histopathology and Pathogenicity. <i>Vaccines</i>, 8(4), http://hdl.handle.net/11427/35229en_ZA
dc.identifier.chicagocitationDouglass, Nicola, Henry Munyanduki, Ruzaiq Omar, Sophette Gers, Paidamwoyo Mutowembwa, Livio Heath, and Anna-Lise Williamson "Influence of the Viral Superoxide Dismutase (SOD) Homologue on Lumpy Skin Disease Virus (LSDV) Growth, Histopathology and Pathogenicity." <i>Vaccines</i> 8, 4. (2020) http://hdl.handle.net/11427/35229en_ZA
dc.identifier.citationDouglass, N., Munyanduki, H., Omar, R., Gers, S., Mutowembwa, P., Heath, L. & Williamson, A. 2020. Influence of the Viral Superoxide Dismutase (SOD) Homologue on Lumpy Skin Disease Virus (LSDV) Growth, Histopathology and Pathogenicity. <i>Vaccines.</i> 8(4) http://hdl.handle.net/11427/35229en_ZA
dc.identifier.risTY - Journal Article AU - Douglass, Nicola AU - Munyanduki, Henry AU - Omar, Ruzaiq AU - Gers, Sophette AU - Mutowembwa, Paidamwoyo AU - Heath, Livio AU - Williamson, Anna-Lise AB - Lumpy skin disease is an important economic disease of cattle that is controlled by vaccination. This paper presents an investigation into the role of the lumpy skin disease virus (LSDV) superoxide dismutase (SOD) homologue on growth and histopathology of the virus both in vitro and in vivo. SOD homologue knock-out and knock-in recombinants (nLSDV∆SOD-UCT and nLSDVSODis-UCT, respectively) were constructed and compared to the Neethling vaccine (nLSDV) for growth in a permissive bovine cell line as well as on fertilized chick chorioallantoic membranes (CAMs). The infected CAMs were scored for histological changes. Deletion of the SOD homologue from LSDV reduced virus growth both in Madin-Darby bovine kidney (MDBK) cells as well as on CAMs. Furthermore, the knockout virus showed reduced inflammation in CAMs and more ballooning degeneration. A pilot experiment was performed in cattle to compare the lesions produced by the different LSDV constructs in the same animal. One animal developed a larger lesion to nLSDV∆SOD-UCT compared to both nLSDVSODis-UCT and nLSDV. Histological analysis of biopsies of these lesions shows less inflammation and necrosis associated with nLSDVSODis-UCT compared to nLSDV and nLSDV∆SOD-UCT. None of the vaccinated animals showed disseminated LSDV disease, indicating that the candidate vaccines are safe for further testing. Our results suggest that the SOD homologue may improve immunogenicity and reduce virulence. DA - 2020-11-07 DB - OpenUCT DP - University of Cape Town IS - 4 J1 - Vaccines LK - https://open.uct.ac.za PY - 2020 T1 - Influence of the Viral Superoxide Dismutase (SOD) Homologue on Lumpy Skin Disease Virus (LSDV) Growth, Histopathology and Pathogenicity TI - Influence of the Viral Superoxide Dismutase (SOD) Homologue on Lumpy Skin Disease Virus (LSDV) Growth, Histopathology and Pathogenicity UR - http://hdl.handle.net/11427/35229 ER -en_ZA
dc.identifier.urihttps://doi.org/10.3390/vaccines8040664
dc.identifier.urihttp://hdl.handle.net/11427/35229
dc.identifier.vancouvercitationDouglass N, Munyanduki H, Omar R, Gers S, Mutowembwa P, Heath L, et al. Influence of the Viral Superoxide Dismutase (SOD) Homologue on Lumpy Skin Disease Virus (LSDV) Growth, Histopathology and Pathogenicity. Vaccines. 2020;8(4) http://hdl.handle.net/11427/35229.en_ZA
dc.language.isoenen_US
dc.publisher.departmentDepartment of Pathologyen_US
dc.publisher.facultyFaculty of Health Sciencesen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.sourceVaccinesen_US
dc.source.journalissue4en_US
dc.source.journalvolume8en_US
dc.source.urihttps://www.mdpi.com/journal/vaccines
dc.titleInfluence of the Viral Superoxide Dismutase (SOD) Homologue on Lumpy Skin Disease Virus (LSDV) Growth, Histopathology and Pathogenicityen_US
dc.typeJournal Articleen_US
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