Acquired immune responses to three malaria vaccine candidates and their relationship to invasion inhibition in two populations naturally exposed to malaria

Simple item page
dc.contributor.authorAddai-Mensah, Otchere
dc.contributor.authorSeidel, Melanie
dc.contributor.authorAmidu, Nafiu
dc.contributor.authorMaskus, Dominika J
dc.contributor.authorKapelski, Stephanie
dc.contributor.authorBreuer, Gudrun
dc.contributor.authorFranken, Carmen
dc.contributor.authorOwusu-Dabo, Ellis
dc.contributor.authorFrempong, Margaret
dc.contributor.authorRakotozandrindrainy, Raphaƫl
dc.contributor.authorSchinkel, Helga
dc.contributor.authorReimann, Andreas
dc.contributor.authorKlockenbring, Torsten
dc.contributor.authorBarth, Stefan
dc.contributor.authorFischer, Rainer
dc.contributor.authorFendel, Rolf
dc.date.accessioned2016-06-07T09:14:06Z
dc.date.available2016-06-07T09:14:06Z
dc.date.issued2016-02-05
dc.date.updated2016-05-18T15:45:04Z
dc.description.abstractBackground: Malaria still represents a major cause of morbidity and mortality predominantly in several developing countries, and remains a priority in many public health programmes. Despite the enormous gains made in control and prevention the development of an effective vaccine represents a persisting challenge. Although several para site antigens including pre-erythrocytic antigens and blood stage antigens have been thoroughly investigated, the identification of solid immune correlates of protection against infection by Plasmodium falciparum or clinical malaria remains a major hurdle. In this study, an immuno-epidemiological survey was carried out between two populations naturally exposed to P. falciparum malaria to determine the immune correlates of protection. Methods: Plasma samples of immune adults from two countries (Ghana and Madagascar) were tested for their reactivity against the merozoite surface proteins MSP1-19, MSP3 and AMA1 by ELISA. The antigens had been selected on the basis of cumulative evidence of their role in anti-malarial immunity. Additionally, reactivity against crude P. falciparum lysate was investigated. Purified IgG from these samples were furthermore tested in an invasion inhibition assay for their antiparasitic activity. Results: Significant intra- and inter- population variation of the reactivity of the samples to the tested antigens were found, as well as a significant positive correlation between MSP1-19 reactivity and invasion inhibition (p < 0.05). Interestingly, male donors showed a significantly higher antibody response to all tested antigens than their female counterparts. In vitro invasion inhibition assays comparing the purified antibodies from the donors from Ghana and Madagascar did not show any statistically significant difference. Although in vitro invasion inhibition increased with breadth of antibody response, the increase was not statistically significant. Conclusions: The findings support the fact that the development of semi-immunity to malaria is probably con tingent on the development of antibodies to not only one, but a range of antigens and that invasion inhibition in immune adults may be a function of antibodies to various antigens. This supports strategies of vaccination including multicomponent vaccines as well as passive vaccination strategies with antibody cocktails.en_ZA
dc.identifier.apacitationAddai-Mensah, O., Seidel, M., Amidu, N., Maskus, D. J., Kapelski, S., Breuer, G., ... Fendel, R. (2016). Acquired immune responses to three malaria vaccine candidates and their relationship to invasion inhibition in two populations naturally exposed to malaria. <i>Malaria Journal</i>, http://hdl.handle.net/11427/19930en_ZA
dc.identifier.chicagocitationAddai-Mensah, Otchere, Melanie Seidel, Nafiu Amidu, Dominika J Maskus, Stephanie Kapelski, Gudrun Breuer, Carmen Franken, et al "Acquired immune responses to three malaria vaccine candidates and their relationship to invasion inhibition in two populations naturally exposed to malaria." <i>Malaria Journal</i> (2016) http://hdl.handle.net/11427/19930en_ZA
dc.identifier.citationAddai-Mensah, O., Seidel, M., Amidu, N., Maskus, D. J., Kapelski, S., Breuer, G., ... & Schinkel, H. (2016). Acquired immune responses to three malaria vaccine candidates and their relationship to invasion inhibition in two populations naturally exposed to malaria. Malaria journal, 15(1), 15.en_ZA
dc.identifier.issn1475-287en_ZA
dc.identifier.ris TY - Journal Article AU - Addai-Mensah, Otchere AU - Seidel, Melanie AU - Amidu, Nafiu AU - Maskus, Dominika J AU - Kapelski, Stephanie AU - Breuer, Gudrun AU - Franken, Carmen AU - Owusu-Dabo, Ellis AU - Frempong, Margaret AU - Rakotozandrindrainy, Raphaƫl AU - Schinkel, Helga AU - Reimann, Andreas AU - Klockenbring, Torsten AU - Barth, Stefan AU - Fischer, Rainer AU - Fendel, Rolf AB - Background: Malaria still represents a major cause of morbidity and mortality predominantly in several developing countries, and remains a priority in many public health programmes. Despite the enormous gains made in control and prevention the development of an effective vaccine represents a persisting challenge. Although several para site antigens including pre-erythrocytic antigens and blood stage antigens have been thoroughly investigated, the identification of solid immune correlates of protection against infection by Plasmodium falciparum or clinical malaria remains a major hurdle. In this study, an immuno-epidemiological survey was carried out between two populations naturally exposed to P. falciparum malaria to determine the immune correlates of protection. Methods: Plasma samples of immune adults from two countries (Ghana and Madagascar) were tested for their reactivity against the merozoite surface proteins MSP1-19, MSP3 and AMA1 by ELISA. The antigens had been selected on the basis of cumulative evidence of their role in anti-malarial immunity. Additionally, reactivity against crude P. falciparum lysate was investigated. Purified IgG from these samples were furthermore tested in an invasion inhibition assay for their antiparasitic activity. Results: Significant intra- and inter- population variation of the reactivity of the samples to the tested antigens were found, as well as a significant positive correlation between MSP1-19 reactivity and invasion inhibition (p < 0.05). Interestingly, male donors showed a significantly higher antibody response to all tested antigens than their female counterparts. In vitro invasion inhibition assays comparing the purified antibodies from the donors from Ghana and Madagascar did not show any statistically significant difference. Although in vitro invasion inhibition increased with breadth of antibody response, the increase was not statistically significant. Conclusions: The findings support the fact that the development of semi-immunity to malaria is probably con tingent on the development of antibodies to not only one, but a range of antigens and that invasion inhibition in immune adults may be a function of antibodies to various antigens. This supports strategies of vaccination including multicomponent vaccines as well as passive vaccination strategies with antibody cocktails. DA - 2016-02-05 DB - OpenUCT DO - 10.1186/s12936-016-1112-1 DP - University of Cape Town J1 - Malaria Journal KW - Plasmodium falciparum KW - Immune response KW - ELISA KW - Invasion inhibition LK - https://open.uct.ac.za PB - University of Cape Town PY - 2016 SM - 1475-287 T1 - Acquired immune responses to three malaria vaccine candidates and their relationship to invasion inhibition in two populations naturally exposed to malaria TI - Acquired immune responses to three malaria vaccine candidates and their relationship to invasion inhibition in two populations naturally exposed to malaria UR - http://hdl.handle.net/11427/19930 ER - en_ZA
dc.identifier.urihttp://dx.doi.org/10.1186/s12936-016-1112-1
dc.identifier.urihttp://hdl.handle.net/11427/19930
dc.identifier.vancouvercitationAddai-Mensah O, Seidel M, Amidu N, Maskus DJ, Kapelski S, Breuer G, et al. Acquired immune responses to three malaria vaccine candidates and their relationship to invasion inhibition in two populations naturally exposed to malaria. Malaria Journal. 2016; http://hdl.handle.net/11427/19930.en_ZA
dc.languageengen_ZA
dc.language.rfc3066en
dc.publisherBioMed Centralen_ZA
dc.publisher.departmentInstitute of Infectious Disease and Molecular Medicineen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsCreative Commons Attribution 4.0 International (CC BY 4.0)*
dc.rights.holderAddai-Mensah et al.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_ZA
dc.sourceMalaria Journalen_ZA
dc.source.urihttp://malariajournal.biomedcentral.com/
dc.subjectPlasmodium falciparum
dc.subjectImmune response
dc.subjectELISA
dc.subjectInvasion inhibition
dc.titleAcquired immune responses to three malaria vaccine candidates and their relationship to invasion inhibition in two populations naturally exposed to malariaen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
12936_2016_Article_1112.pdf
Size:
1.44 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.72 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Journal Articles