Evaluation of five bifidobacterium isolates as potential probiotics and genetic analysis of their ability to withstand oxidative stress

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dc.contributor.advisorReid, Sharon Jen_ZA
dc.contributor.advisorAbratt, Valerie Roseen_ZA
dc.contributor.authorNonyane, Molati Alberten_ZA
dc.date.accessioned2015-01-03T18:14:45Z
dc.date.available2015-01-03T18:14:45Z
dc.date.issued2010en_ZA
dc.descriptionIncludes bibliographical references (p. 102-112).en_ZA
dc.description.abstractThe diverse microbiota of the human gastrointestinal tract plays a major role in the general health of humans. A number of bacterial strains with distinctive properties have been isolated and used commercially as probiotics in order to harness these health benefits and offer them to unhealthy hosts. There are set criteria that have to be followed before new probiotics can be introduced into the market. Five Bifidobacterium isolates with extremely high sucrase activity were randomly selected from a faecal sample from a healthy donor for further characterisation as potential probiotics with the ability to utilize fructo- oligosaccharide substrates in the gut. Phylogenic identification of the isolates to the species level was carried out using sequences of the 16S rRNA gene, 16S-23S rRNA gene spacer region, the heat shock protein (hsp60) and Elongation factor Tu (tuf).en_ZA
dc.identifier.apacitationNonyane, M. A. (2010). <i>Evaluation of five bifidobacterium isolates as potential probiotics and genetic analysis of their ability to withstand oxidative stress</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/11175en_ZA
dc.identifier.chicagocitationNonyane, Molati Albert. <i>"Evaluation of five bifidobacterium isolates as potential probiotics and genetic analysis of their ability to withstand oxidative stress."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2010. http://hdl.handle.net/11427/11175en_ZA
dc.identifier.citationNonyane, M. 2010. Evaluation of five bifidobacterium isolates as potential probiotics and genetic analysis of their ability to withstand oxidative stress. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Nonyane, Molati Albert AB - The diverse microbiota of the human gastrointestinal tract plays a major role in the general health of humans. A number of bacterial strains with distinctive properties have been isolated and used commercially as probiotics in order to harness these health benefits and offer them to unhealthy hosts. There are set criteria that have to be followed before new probiotics can be introduced into the market. Five Bifidobacterium isolates with extremely high sucrase activity were randomly selected from a faecal sample from a healthy donor for further characterisation as potential probiotics with the ability to utilize fructo- oligosaccharide substrates in the gut. Phylogenic identification of the isolates to the species level was carried out using sequences of the 16S rRNA gene, 16S-23S rRNA gene spacer region, the heat shock protein (hsp60) and Elongation factor Tu (tuf). DA - 2010 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2010 T1 - Evaluation of five bifidobacterium isolates as potential probiotics and genetic analysis of their ability to withstand oxidative stress TI - Evaluation of five bifidobacterium isolates as potential probiotics and genetic analysis of their ability to withstand oxidative stress UR - http://hdl.handle.net/11427/11175 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/11175
dc.identifier.vancouvercitationNonyane MA. Evaluation of five bifidobacterium isolates as potential probiotics and genetic analysis of their ability to withstand oxidative stress. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2010 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/11175en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherCell Biologyen_ZA
dc.titleEvaluation of five bifidobacterium isolates as potential probiotics and genetic analysis of their ability to withstand oxidative stressen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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