Characterisation of the population genetics of farm-bred Haliotis midae using microsatellite DNA markers

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dc.contributor.advisorCoyne, Vernonen_ZA
dc.contributor.authorNaidoo, René Kathleenen_ZA
dc.date.accessioned2014-07-30T17:40:26Z
dc.date.available2014-07-30T17:40:26Z
dc.date.issued2006en_ZA
dc.descriptionIncludes bibliographical references (leaves 89-94).
dc.description.abstractThe abalone Haliotis midae is a gastropod mollusc which is of commercial importance in South Africa due to its high export value. During this study the genetic structure of farmed Haliotis midae was investigated in order to determine whether microsatellite DNA analysis could be used to separate farmed abalone into phenotypically different groups with respect to growth rate. Microsatellites display high levels of variability and this makes them suitable for a wide variety of applications in aquaculture, particularly where genetic differentiation between population groups may be limited. This study investigates the genetic composition of 120 individuals from 2 spawning events that had been classed as either fast or slow growing by the farm managers. Three highly polymorphic microsatellite loci were selected and used to determine the extent of the genetic diversity which exists amongst the 120 individuals tested from the lacobsbaai abalone farm. Additionally, these microsatellite loci were used to determine whether abalone classed as either fast or slow growing could be differentiated into specific genetic population groups. Neighbour-joining trees constructed using genetic distance data obtained for all three loci demonstrated that a distinct separation between the fast and slow growing abalone was evident. It was also found that there has been a loss of genetic diversity on the lacobsbaai abalone farm in terms of heterozygosity. This has been attributed to the high levels of inbreeding as evidenced by the high Fis values. All population groups were found to deviate significantly from Hardy-Weinberg equilibrium and this is most likely due to the occurrence of non-random mating on the lacobsbaai abalone farm. This study has successfully demonstrated that a combination of micro satellite loci with high allelic diversity can potentially be employed as a tool for distinguishing between fast and slow growing farmed abalone. This study needs to be validated by testing larger sample sizes and the use of additional farms.en_ZA
dc.identifier.apacitationNaidoo, R. K. (2006). <i>Characterisation of the population genetics of farm-bred Haliotis midae using microsatellite DNA markers</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/4307en_ZA
dc.identifier.chicagocitationNaidoo, René Kathleen. <i>"Characterisation of the population genetics of farm-bred Haliotis midae using microsatellite DNA markers."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2006. http://hdl.handle.net/11427/4307en_ZA
dc.identifier.citationNaidoo, R. 2006. Characterisation of the population genetics of farm-bred Haliotis midae using microsatellite DNA markers. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Naidoo, René Kathleen AB - The abalone Haliotis midae is a gastropod mollusc which is of commercial importance in South Africa due to its high export value. During this study the genetic structure of farmed Haliotis midae was investigated in order to determine whether microsatellite DNA analysis could be used to separate farmed abalone into phenotypically different groups with respect to growth rate. Microsatellites display high levels of variability and this makes them suitable for a wide variety of applications in aquaculture, particularly where genetic differentiation between population groups may be limited. This study investigates the genetic composition of 120 individuals from 2 spawning events that had been classed as either fast or slow growing by the farm managers. Three highly polymorphic microsatellite loci were selected and used to determine the extent of the genetic diversity which exists amongst the 120 individuals tested from the lacobsbaai abalone farm. Additionally, these microsatellite loci were used to determine whether abalone classed as either fast or slow growing could be differentiated into specific genetic population groups. Neighbour-joining trees constructed using genetic distance data obtained for all three loci demonstrated that a distinct separation between the fast and slow growing abalone was evident. It was also found that there has been a loss of genetic diversity on the lacobsbaai abalone farm in terms of heterozygosity. This has been attributed to the high levels of inbreeding as evidenced by the high Fis values. All population groups were found to deviate significantly from Hardy-Weinberg equilibrium and this is most likely due to the occurrence of non-random mating on the lacobsbaai abalone farm. This study has successfully demonstrated that a combination of micro satellite loci with high allelic diversity can potentially be employed as a tool for distinguishing between fast and slow growing farmed abalone. This study needs to be validated by testing larger sample sizes and the use of additional farms. DA - 2006 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2006 T1 - Characterisation of the population genetics of farm-bred Haliotis midae using microsatellite DNA markers TI - Characterisation of the population genetics of farm-bred Haliotis midae using microsatellite DNA markers UR - http://hdl.handle.net/11427/4307 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/4307
dc.identifier.vancouvercitationNaidoo RK. Characterisation of the population genetics of farm-bred Haliotis midae using microsatellite DNA markers. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2006 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/4307en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherCell Biologyen_ZA
dc.titleCharacterisation of the population genetics of farm-bred Haliotis midae using microsatellite DNA markersen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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