CD146 expression in early and late onset pre-eclampsia : is there a difference?

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dc.contributor.advisorWainwright, Helenen_ZA
dc.contributor.advisorWright, Colleen Aen_ZA
dc.contributor.authorSchubert, Pawel Tomaszen_ZA
dc.date.accessioned2015-07-03T08:29:09Z
dc.date.available2015-07-03T08:29:09Z
dc.date.issued2014en_ZA
dc.descriptionIncludes bibliographical references.en_ZA
dc.description.abstractObjective: To investigate the difference in expression of CD146 immunohistochemical staining on intermediate trophoblast in early and late onset pre-eclampsia placentas as well as comparing this expression to gestational age matched control placentas. Study Design: Retrospective case series of 100 placentas: 25 early onset and 25 late onset pre-eclampsia placentas as well as 25 early and 25 late gestational age matched control placentas. Placentas were obtained from patients delivering in Tygerberg Hospital. Methods: Placentas were routinely fixed and processed. 2 sections of one preselected block from each case was cut and stained with CD146 and MNF116 immunohistochemical stain. The expression of the staining would be performed by means of analytical and image analysing software. Results: The study failed to demonstrate differences in CD146 expression by the intermediate trophoblast in the pre-eclampsia and control placentas. The analytical approach was deemed to be subjective and the image analysing software had too much background staining and inaccurate identification of the intermediate trophoblast in order to produce reproducible consistent results. Conclusion: Dual staining, using immunofluorescent staining of CD146 and MNF116 on smaller biopsies of the decidua are thought to be able to produce much better material for image analyses software.en_ZA
dc.identifier.apacitationSchubert, P. T. (2014). <i>CD146 expression in early and late onset pre-eclampsia : is there a difference?</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Anatomical Pathology. Retrieved from http://hdl.handle.net/11427/13337en_ZA
dc.identifier.chicagocitationSchubert, Pawel Tomasz. <i>"CD146 expression in early and late onset pre-eclampsia : is there a difference?."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Anatomical Pathology, 2014. http://hdl.handle.net/11427/13337en_ZA
dc.identifier.citationSchubert, P. 2014. CD146 expression in early and late onset pre-eclampsia : is there a difference?. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Schubert, Pawel Tomasz AB - Objective: To investigate the difference in expression of CD146 immunohistochemical staining on intermediate trophoblast in early and late onset pre-eclampsia placentas as well as comparing this expression to gestational age matched control placentas. Study Design: Retrospective case series of 100 placentas: 25 early onset and 25 late onset pre-eclampsia placentas as well as 25 early and 25 late gestational age matched control placentas. Placentas were obtained from patients delivering in Tygerberg Hospital. Methods: Placentas were routinely fixed and processed. 2 sections of one preselected block from each case was cut and stained with CD146 and MNF116 immunohistochemical stain. The expression of the staining would be performed by means of analytical and image analysing software. Results: The study failed to demonstrate differences in CD146 expression by the intermediate trophoblast in the pre-eclampsia and control placentas. The analytical approach was deemed to be subjective and the image analysing software had too much background staining and inaccurate identification of the intermediate trophoblast in order to produce reproducible consistent results. Conclusion: Dual staining, using immunofluorescent staining of CD146 and MNF116 on smaller biopsies of the decidua are thought to be able to produce much better material for image analyses software. DA - 2014 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2014 T1 - CD146 expression in early and late onset pre-eclampsia : is there a difference? TI - CD146 expression in early and late onset pre-eclampsia : is there a difference? UR - http://hdl.handle.net/11427/13337 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/13337
dc.identifier.vancouvercitationSchubert PT. CD146 expression in early and late onset pre-eclampsia : is there a difference?. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Anatomical Pathology, 2014 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/13337en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Anatomical Pathologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherPaediatric Pathologyen_ZA
dc.titleCD146 expression in early and late onset pre-eclampsia : is there a difference?en_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMPhilen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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