Defence responses of Arabidopsis thaliana to infection by Pseudomonas syringae are regulated by the circadian clock

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dc.contributor.authorBhardwaj, Vaibhaven_ZA
dc.contributor.authorMeier, Stuarten_ZA
dc.contributor.authorPetersen, Lindsay Nen_ZA
dc.contributor.authorIngle, Robert Aen_ZA
dc.contributor.authorRoden, Laura Cen_ZA
dc.date.accessioned2015-11-16T04:12:29Z
dc.date.available2015-11-16T04:12:29Z
dc.date.issued2011en_ZA
dc.description.abstractThe circadian clock allows plants to anticipate predictable daily changes in abiotic stimuli, such as light; however, whether the clock similarly allows plants to anticipate interactions with other organisms is unknown. Here we show that Arabidopsis thaliana (Arabidopsis) has circadian clock-mediated variation in resistance to the virulent bacterial pathogen Pseudomonas syringae pv. tomato DC3000 ( Pst DC3000), with plants being least susceptible to infection in the subjective morning. We suggest that the increased resistance to Pst DC3000 observed in the morning in Col-0 plants results from clock-mediated modulation of pathogen associated molecular pattern (PAMP)-triggered immunity. Analysis of publicly available microarray data revealed that a large number of Arabidopsis defence-related genes showed both diurnal- and circadian-regulation, including genes involved in the perception of the PAMP flagellin which exhibit a peak in expression in the morning. Accordingly, we observed that PAMP-triggered callose deposition was significantly higher in wild-type plants inoculated with Pst DC3000 hrpA in the subjective morning than in the evening, while no such temporal difference was evident in arrhythmic plants. Our results suggest that PAMP-triggered immune responses are modulated by the circadian clock and that temporal regulation allows plants to anticipate and respond more effectively to pathogen challenges in the daytime.en_ZA
dc.identifier.apacitationBhardwaj, V., Meier, S., Petersen, L. N., Ingle, R. A., & Roden, L. C. (2011). Defence responses of Arabidopsis thaliana to infection by Pseudomonas syringae are regulated by the circadian clock. <i>PLoS One</i>, http://hdl.handle.net/11427/15025en_ZA
dc.identifier.chicagocitationBhardwaj, Vaibhav, Stuart Meier, Lindsay N Petersen, Robert A Ingle, and Laura C Roden "Defence responses of Arabidopsis thaliana to infection by Pseudomonas syringae are regulated by the circadian clock." <i>PLoS One</i> (2011) http://hdl.handle.net/11427/15025en_ZA
dc.identifier.citationBhardwaj, V., Meier, S., Petersen, L. N., Ingle, R. A., & Roden, L. C. (2011). Defence responses of Arabidopsis thaliana to infection by Pseudomonas syringae are regulated by the circadian clock. PloS one, 6(10), e26968-e26968. doi:10.1371/journal.pone.0026968en_ZA
dc.identifier.ris TY - Journal Article AU - Bhardwaj, Vaibhav AU - Meier, Stuart AU - Petersen, Lindsay N AU - Ingle, Robert A AU - Roden, Laura C AB - The circadian clock allows plants to anticipate predictable daily changes in abiotic stimuli, such as light; however, whether the clock similarly allows plants to anticipate interactions with other organisms is unknown. Here we show that Arabidopsis thaliana (Arabidopsis) has circadian clock-mediated variation in resistance to the virulent bacterial pathogen Pseudomonas syringae pv. tomato DC3000 ( Pst DC3000), with plants being least susceptible to infection in the subjective morning. We suggest that the increased resistance to Pst DC3000 observed in the morning in Col-0 plants results from clock-mediated modulation of pathogen associated molecular pattern (PAMP)-triggered immunity. Analysis of publicly available microarray data revealed that a large number of Arabidopsis defence-related genes showed both diurnal- and circadian-regulation, including genes involved in the perception of the PAMP flagellin which exhibit a peak in expression in the morning. Accordingly, we observed that PAMP-triggered callose deposition was significantly higher in wild-type plants inoculated with Pst DC3000 hrpA in the subjective morning than in the evening, while no such temporal difference was evident in arrhythmic plants. Our results suggest that PAMP-triggered immune responses are modulated by the circadian clock and that temporal regulation allows plants to anticipate and respond more effectively to pathogen challenges in the daytime. DA - 2011 DB - OpenUCT DO - 10.1371/journal.pone.0026968 DP - University of Cape Town J1 - PLoS One LK - https://open.uct.ac.za PB - University of Cape Town PY - 2011 T1 - Defence responses of Arabidopsis thaliana to infection by Pseudomonas syringae are regulated by the circadian clock TI - Defence responses of Arabidopsis thaliana to infection by Pseudomonas syringae are regulated by the circadian clock UR - http://hdl.handle.net/11427/15025 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/15025
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pone.0026968
dc.identifier.vancouvercitationBhardwaj V, Meier S, Petersen LN, Ingle RA, Roden LC. Defence responses of Arabidopsis thaliana to infection by Pseudomonas syringae are regulated by the circadian clock. PLoS One. 2011; http://hdl.handle.net/11427/15025.en_ZA
dc.language.isoengen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_ZA
dc.rights.holder© 2011 Bhardwaj et alen_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/4.0en_ZA
dc.sourcePLoS Oneen_ZA
dc.source.urihttp://journals.plos.org/plosoneen_ZA
dc.subject.otherCircadian rhythmsen_ZA
dc.subject.otherCircadian oscillatorsen_ZA
dc.subject.otherGene regulationen_ZA
dc.subject.otherGene expressionen_ZA
dc.subject.otherPlant pathogensen_ZA
dc.subject.otherPlant bacterial pathogensen_ZA
dc.subject.otherPseudomonas infectionsen_ZA
dc.subject.otherArabidopsis thalianaen_ZA
dc.titleDefence responses of Arabidopsis thaliana to infection by Pseudomonas syringae are regulated by the circadian clocken_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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