Studies on in vitro antiplasmodial activity of cleome rutidosperma

dc.contributor.authorBose, Anindya
dc.contributor.authorLategan, Carmen Abriette
dc.contributor.authorSmith, Peter J
dc.contributor.authorGupta, Jayanta Kumar
dc.date.accessioned2018-06-06T09:56:22Z
dc.date.available2018-06-06T09:56:22Z
dc.date.issued2010
dc.date.updated2016-01-20T09:07:01Z
dc.description.abstractMalaria is a protozoal disease transmitted by the Anopheles mosquito, caused by minute parasitic protozoa of the genus Plasmodium, which infect human and insect hosts alternatively. It affects over 40% of the worldís population, with 120 million cases reported, and about 2 million deaths annually (1). The P. falciparum variety of the parasite accounts for 80% of cases and 90% of deaths caused by malaria. The declining efficacy of classical medication in relation to the rapid increase of parasite resistant strains, mainly of Plasmodium falciparum, as well as the greater resistance of vectors to insecticides, and the difficulty of creating efficient vaccines have led to an urgent need for new efficient antimalarial drugs (2, 3). Natural molecules may provide innovative strategies towards malarial control, hence active research groups are now working to develop new active compounds as an alternative to chloroquine, especially from artemisinin (4, 5), a plant-based antimalaria drug isolated from the Chinese plant Artemisia annua (6). Plants may well, therefore, prove to be the sources of new antimalarial in view of the success with the two important chemotherapeutic agents, quinine and artemisinin, both of which are derived from plants. Cleome rutidosperma (Capparidaceae) is a low-growing herb, up to 70 cm tall, found in waste grounds and grassy places with trifoliate leaves and small, violet-blue flowers, which turn pink as they age. The elongated capsules display the asymmetrical, dull black seeds. The plant is native to West Africa, although it has become naturalized in various parts of tropical America as well as Southeast Asia (7, 8). The diuretic, laxative, anthelmintic, antimicrobial, analgesic, anti-inflammatory, antipyretic, antioxidant and free radical scavenging activities of Cleome rutidosperma were reported earlier by the authors (9-13). The plant is used as antimalarial by the traditional healers in Cameroon and mild antiplasmodial activity of chloroform/methanol (1:1) extract of leaves of Cleome rutidosperma against chloroquine-sensitive (F32) laboratory strain of P. falciparum was reported earlier in Cameroon (14). The present study investigates the in vitro antiplasmodial activity of ethanolic extract and its fractions of aerial parts of Cleome rutidosperma against the chloroquine sensitive (CQS) D10 strain of the parasite, as well as their toxicity against a mammalian cell lines.
dc.identifier.apacitation 2010. <i>Studies on in vitro antiplasmodial activity of cleome rutidosperma.</i> http://hdl.handle.net/11427/28233en_ZA
dc.identifier.chicagocitation. 2010. <i>Studies on in vitro antiplasmodial activity of cleome rutidosperma.</i> http://hdl.handle.net/11427/28233en_ZA
dc.identifier.citationBose, A., Smith, P. J., Lategan, C. A., Gupta, J. K., & Si, S. (2010). Studies on in vitro antiplasmodial activity of Cleome rutidosperma. Acta Polanica Pharm Drug Res, 67, 315-8.
dc.identifier.ris TY - AU - Bose, Anindya AU - Lategan, Carmen Abriette AU - Smith, Peter J AU - Gupta, Jayanta Kumar AB - Malaria is a protozoal disease transmitted by the Anopheles mosquito, caused by minute parasitic protozoa of the genus Plasmodium, which infect human and insect hosts alternatively. It affects over 40% of the worldís population, with 120 million cases reported, and about 2 million deaths annually (1). The P. falciparum variety of the parasite accounts for 80% of cases and 90% of deaths caused by malaria. The declining efficacy of classical medication in relation to the rapid increase of parasite resistant strains, mainly of Plasmodium falciparum, as well as the greater resistance of vectors to insecticides, and the difficulty of creating efficient vaccines have led to an urgent need for new efficient antimalarial drugs (2, 3). Natural molecules may provide innovative strategies towards malarial control, hence active research groups are now working to develop new active compounds as an alternative to chloroquine, especially from artemisinin (4, 5), a plant-based antimalaria drug isolated from the Chinese plant Artemisia annua (6). Plants may well, therefore, prove to be the sources of new antimalarial in view of the success with the two important chemotherapeutic agents, quinine and artemisinin, both of which are derived from plants. Cleome rutidosperma (Capparidaceae) is a low-growing herb, up to 70 cm tall, found in waste grounds and grassy places with trifoliate leaves and small, violet-blue flowers, which turn pink as they age. The elongated capsules display the asymmetrical, dull black seeds. The plant is native to West Africa, although it has become naturalized in various parts of tropical America as well as Southeast Asia (7, 8). The diuretic, laxative, anthelmintic, antimicrobial, analgesic, anti-inflammatory, antipyretic, antioxidant and free radical scavenging activities of Cleome rutidosperma were reported earlier by the authors (9-13). The plant is used as antimalarial by the traditional healers in Cameroon and mild antiplasmodial activity of chloroform/methanol (1:1) extract of leaves of Cleome rutidosperma against chloroquine-sensitive (F32) laboratory strain of P. falciparum was reported earlier in Cameroon (14). The present study investigates the in vitro antiplasmodial activity of ethanolic extract and its fractions of aerial parts of Cleome rutidosperma against the chloroquine sensitive (CQS) D10 strain of the parasite, as well as their toxicity against a mammalian cell lines. DA - 2010 DB - OpenUCT DP - University of Cape Town J1 - Acta Poloniae Pharmaceutica LK - https://open.uct.ac.za PB - University of Cape Town PY - 2010 T1 - Studies on in vitro antiplasmodial activity of cleome rutidosperma TI - Studies on in vitro antiplasmodial activity of cleome rutidosperma UR - http://hdl.handle.net/11427/28233 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/28233
dc.identifier.vancouvercitation. 2010. <i>Studies on in vitro antiplasmodial activity of cleome rutidosperma.</i> http://hdl.handle.net/11427/28233en_ZA
dc.language.isoeng
dc.publisher.departmentDivision of Clinical Pharmacologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.sourceActa Poloniae Pharmaceutica
dc.subject.otherCleome rutidosperma
dc.subject.otherPlasmodium falciparum
dc.subject.otheraqueous extract
dc.subject.otherethanolic extract
dc.subject.otherfractionation
dc.subject.othermalaria
dc.subject.otherantiplasmodial activity
dc.subject.othercytotoxicity
dc.titleStudies on in vitro antiplasmodial activity of cleome rutidosperma
dc.typeOther
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