The production of a Crimean-Congo haemorrhagic fever virus diagnostic antigen in plants

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dc.contributor.advisorMeyers, Annen_ZA
dc.contributor.advisorRybicki, Edward Pen_ZA
dc.contributor.authorAtkinson, Richarden_ZA
dc.date.accessioned2016-07-20T06:51:44Z
dc.date.available2016-07-20T06:51:44Z
dc.date.issued2016en_ZA
dc.description.abstractCrimean-­Congo Hemorrhagic Fever (CCHF) is a highly infectious, life threatening disease, caused by the Crimean-­Congo Hemorrhagic Fever Virus (CCHFV), a nairovirus that forms part of the Bunyaviridae family. CCHFV has a case fatality rate of approximately 40%. Current diagnostic methods for CCHFV involve the use of live virus antigen, requiring biosafety level 4 (BSL4) conditions for safe handling. The development of a safer diagnostic reagent for detection of this disease is therefore desirable. This project involves the development of a recombinant CCHFV nucleocapsid protein (NP). The nucleocapsid (NP) protein was expressed in Nicotiana benthamiana and purified using a 6x histidine-­tag. The protein was then reacted against serum samples collected from confirmed CCHFV patients to determine its ability to detect IgG antibodies against CCHFV in human sera.en_ZA
dc.identifier.apacitationAtkinson, R. (2016). <i>The production of a Crimean-Congo haemorrhagic fever virus diagnostic antigen in plants</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/20474en_ZA
dc.identifier.chicagocitationAtkinson, Richard. <i>"The production of a Crimean-Congo haemorrhagic fever virus diagnostic antigen in plants."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2016. http://hdl.handle.net/11427/20474en_ZA
dc.identifier.citationAtkinson, R. 2016. The production of a Crimean-Congo haemorrhagic fever virus diagnostic antigen in plants. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Atkinson, Richard AB - Crimean-­Congo Hemorrhagic Fever (CCHF) is a highly infectious, life threatening disease, caused by the Crimean-­Congo Hemorrhagic Fever Virus (CCHFV), a nairovirus that forms part of the Bunyaviridae family. CCHFV has a case fatality rate of approximately 40%. Current diagnostic methods for CCHFV involve the use of live virus antigen, requiring biosafety level 4 (BSL4) conditions for safe handling. The development of a safer diagnostic reagent for detection of this disease is therefore desirable. This project involves the development of a recombinant CCHFV nucleocapsid protein (NP). The nucleocapsid (NP) protein was expressed in Nicotiana benthamiana and purified using a 6x histidine-­tag. The protein was then reacted against serum samples collected from confirmed CCHFV patients to determine its ability to detect IgG antibodies against CCHFV in human sera. DA - 2016 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2016 T1 - The production of a Crimean-Congo haemorrhagic fever virus diagnostic antigen in plants TI - The production of a Crimean-Congo haemorrhagic fever virus diagnostic antigen in plants UR - http://hdl.handle.net/11427/20474 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/20474
dc.identifier.vancouvercitationAtkinson R. The production of a Crimean-Congo haemorrhagic fever virus diagnostic antigen in plants. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2016 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/20474en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMolecular and Cell Biologyen_ZA
dc.titleThe production of a Crimean-Congo haemorrhagic fever virus diagnostic antigen in plantsen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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