Stability studies of HIV-1 Pr55gag virus-like particles made in insect cells after storage in various formulation media

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dc.contributor.authorLynch, Alissonen_ZA
dc.contributor.authorMeyers, Annen_ZA
dc.contributor.authorWilliamson, Anna-Liseen_ZA
dc.contributor.authorRybicki, Edwarden_ZA
dc.date.accessioned2015-11-18T03:59:19Z
dc.date.available2015-11-18T03:59:19Z
dc.date.issued2012en_ZA
dc.description.abstractBACKGROUND:HIV-1 Pr55gag virus-like particles (VLPs) expressed by baculovirus in insect cells are considered to be a very promising HIV-1 vaccine candidate, as they have been shown to elicit broad cellular immune responses when tested in animals, particularly when used as a boost to DNA or BCG vaccines. However, it is important for the VLPs to retain their structure for them to be fully functional and effective. The medium in which the VLPs are formulated and the temperature at which they are stored are two important factors affecting their stability.FINDINGS:We describe the screening of 3 different readily available formulation media (sorbitol, sucrose and trehalose) for their ability to stabilise HIV-1 Pr55gag VLPs during prolonged storage. Transmission electron microscopy (TEM) was done on VLPs stored at two different concentrations of the media at three different temperatures (4degreesC, -20degreesC and 70degreesC) over different time periods, and the appearance of the VLPs was compared. VLPs stored in 15% trehalose at 70degreesC retained their original appearance the most effectively over a period of 12 months. VLPs stored in 5% trehalose, sorbitol or sucrose were not all intact even after 1 month storage at the temperatures tested. In addition, we showed that VLPs stored under these conditions were able to be frozen and re-thawed twice before showing changes in their appearance. CONCLUSIONS: Although the inclusion of other analytical tools are essential to validate these preliminary findings, storage in 15% trehalose at 70degreesC for 12 months is most effective in retaining VLP stability.en_ZA
dc.identifier.apacitationLynch, A., Meyers, A., Williamson, A., & Rybicki, E. (2012). Stability studies of HIV-1 Pr55gag virus-like particles made in insect cells after storage in various formulation media. <i>Virology Journal</i>, http://hdl.handle.net/11427/15088en_ZA
dc.identifier.chicagocitationLynch, Alisson, Ann Meyers, Anna-Lise Williamson, and Edward Rybicki "Stability studies of HIV-1 Pr55gag virus-like particles made in insect cells after storage in various formulation media." <i>Virology Journal</i> (2012) http://hdl.handle.net/11427/15088en_ZA
dc.identifier.citationLynch, A., Meyers, A. E., Williamson, A. L., & Rybicki, E. P. (2012). Stability studies of HIV-1 Pr55gag virus-like particles made in insect cells after storage in various formulation media. Virol J, 9(1), 210.en_ZA
dc.identifier.ris TY - Journal Article AU - Lynch, Alisson AU - Meyers, Ann AU - Williamson, Anna-Lise AU - Rybicki, Edward AB - BACKGROUND:HIV-1 Pr55gag virus-like particles (VLPs) expressed by baculovirus in insect cells are considered to be a very promising HIV-1 vaccine candidate, as they have been shown to elicit broad cellular immune responses when tested in animals, particularly when used as a boost to DNA or BCG vaccines. However, it is important for the VLPs to retain their structure for them to be fully functional and effective. The medium in which the VLPs are formulated and the temperature at which they are stored are two important factors affecting their stability.FINDINGS:We describe the screening of 3 different readily available formulation media (sorbitol, sucrose and trehalose) for their ability to stabilise HIV-1 Pr55gag VLPs during prolonged storage. Transmission electron microscopy (TEM) was done on VLPs stored at two different concentrations of the media at three different temperatures (4degreesC, -20degreesC and 70degreesC) over different time periods, and the appearance of the VLPs was compared. VLPs stored in 15% trehalose at 70degreesC retained their original appearance the most effectively over a period of 12 months. VLPs stored in 5% trehalose, sorbitol or sucrose were not all intact even after 1 month storage at the temperatures tested. In addition, we showed that VLPs stored under these conditions were able to be frozen and re-thawed twice before showing changes in their appearance. CONCLUSIONS: Although the inclusion of other analytical tools are essential to validate these preliminary findings, storage in 15% trehalose at 70degreesC for 12 months is most effective in retaining VLP stability. DA - 2012 DB - OpenUCT DO - 10.1186/1743-422X-9-210 DP - University of Cape Town J1 - Virology Journal LK - https://open.uct.ac.za PB - University of Cape Town PY - 2012 T1 - Stability studies of HIV-1 Pr55gag virus-like particles made in insect cells after storage in various formulation media TI - Stability studies of HIV-1 Pr55gag virus-like particles made in insect cells after storage in various formulation media UR - http://hdl.handle.net/11427/15088 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/15088
dc.identifier.urihttp://dx.doi.org/10.1186/1743-422X-9-210
dc.identifier.vancouvercitationLynch A, Meyers A, Williamson A, Rybicki E. Stability studies of HIV-1 Pr55gag virus-like particles made in insect cells after storage in various formulation media. Virology Journal. 2012; http://hdl.handle.net/11427/15088.en_ZA
dc.language.isoengen_ZA
dc.publisherBioMed Central Ltden_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution Licenseen_ZA
dc.rights.holderLynch et al.; licensee BioMed Central Ltd. 2012en_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en_ZA
dc.sourceVirology Journalen_ZA
dc.source.urihttp://virologyj.biomedcentral.com/en_ZA
dc.subject.otherHuman immunodeficiency virusen_ZA
dc.subject.otherPr55gagen_ZA
dc.subject.otherVirus-like particlesen_ZA
dc.subject.otherOsmolytesen_ZA
dc.subject.otherThermostabilityen_ZA
dc.subject.otherHIV vaccineen_ZA
dc.titleStability studies of HIV-1 Pr55gag virus-like particles made in insect cells after storage in various formulation mediaen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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