Induction of the retinal pigment epithelium of the chicken embryonic eye

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dc.contributor.advisorKidson, Sueen_ZA
dc.contributor.authorFranz, Tamara Anneen_ZA
dc.date.accessioned2018-01-25T13:59:17Z
dc.date.available2018-01-25T13:59:17Z
dc.date.issued1997en_ZA
dc.description.abstractDuring development of the eye, invagination of the optic cup gives rise to a double layered neuroepithelium, part of which differentiates into the retinal pigment epithelium (RPE). The molecular mechanisms which control differentiation of the RPE are not known. The present study was undertaken to determine 1) when induction of the RPE has occurred in chicken embryos and 2) to investigate whether contact with the presumptive neural retina (NR) is required for RPE differentiation. In order to investigate when RPE induction has occurred, early expression of two genes involved in pigmentation were investigated. Digoxigenin-labeled tyrosinase and tyrosinaserelated protein-2 (TRP-2) riboprobes were synthesised and used in ISH reactions on embryonic eye tissue. Tyrosinase transcripts were first detected at stage 19.5 (70-71 hours) and TRP-2 transcripts were detected a few hours earlier at stage 18.5 (67-69 hours) of embryonic development. These results indicate that induction has occurred by stage 18.5, approximately ten hours before distinct granules are visible in the RPE. The tyrosinase and TRP-2 transcripts were always localised first in the optical axis of the eye in the region where pigment granules are first present. This indicates that differentiation of the RPE proceeds from the optical axis of the eye cup outwards towards the periphery and that induction of the RPE may also proceed in this direction. To determine whether the presumptive NR is required for RPE induction, synthetic barriers were inserted into the uninvaginated optic vesicle of chicken embryos at stage 11 (40-45 hours) of development. The embryos were cultured in vitro until the optic vesicle had invaginated and sectioned to locate the barrier. Results suggest that contact with the presumptive NR may not be necessary for RPE induction.en_ZA
dc.identifier.apacitationFranz, T. A. (1997). <i>Induction of the retinal pigment epithelium of the chicken embryonic eye</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Cell Biology. Retrieved from http://hdl.handle.net/11427/26993en_ZA
dc.identifier.chicagocitationFranz, Tamara Anne. <i>"Induction of the retinal pigment epithelium of the chicken embryonic eye."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Cell Biology, 1997. http://hdl.handle.net/11427/26993en_ZA
dc.identifier.citationFranz, T. 1997. Induction of the retinal pigment epithelium of the chicken embryonic eye. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Franz, Tamara Anne AB - During development of the eye, invagination of the optic cup gives rise to a double layered neuroepithelium, part of which differentiates into the retinal pigment epithelium (RPE). The molecular mechanisms which control differentiation of the RPE are not known. The present study was undertaken to determine 1) when induction of the RPE has occurred in chicken embryos and 2) to investigate whether contact with the presumptive neural retina (NR) is required for RPE differentiation. In order to investigate when RPE induction has occurred, early expression of two genes involved in pigmentation were investigated. Digoxigenin-labeled tyrosinase and tyrosinaserelated protein-2 (TRP-2) riboprobes were synthesised and used in ISH reactions on embryonic eye tissue. Tyrosinase transcripts were first detected at stage 19.5 (70-71 hours) and TRP-2 transcripts were detected a few hours earlier at stage 18.5 (67-69 hours) of embryonic development. These results indicate that induction has occurred by stage 18.5, approximately ten hours before distinct granules are visible in the RPE. The tyrosinase and TRP-2 transcripts were always localised first in the optical axis of the eye in the region where pigment granules are first present. This indicates that differentiation of the RPE proceeds from the optical axis of the eye cup outwards towards the periphery and that induction of the RPE may also proceed in this direction. To determine whether the presumptive NR is required for RPE induction, synthetic barriers were inserted into the uninvaginated optic vesicle of chicken embryos at stage 11 (40-45 hours) of development. The embryos were cultured in vitro until the optic vesicle had invaginated and sectioned to locate the barrier. Results suggest that contact with the presumptive NR may not be necessary for RPE induction. DA - 1997 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1997 T1 - Induction of the retinal pigment epithelium of the chicken embryonic eye TI - Induction of the retinal pigment epithelium of the chicken embryonic eye UR - http://hdl.handle.net/11427/26993 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/26993
dc.identifier.vancouvercitationFranz TA. Induction of the retinal pigment epithelium of the chicken embryonic eye. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Cell Biology, 1997 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/26993en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Cell Biologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherCell Biologyen_ZA
dc.titleInduction of the retinal pigment epithelium of the chicken embryonic eyeen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMSc (Med)en_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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